MLVA GENOTYPING OF B. ANTHRACIS IN THE FORMATION OF A COLLECTION OF REPRESENTATIVE STRAINS
Main Article Content
Authors
T.B. Karibaev
National Reference Center for Veterinary Medicine, 22/3, 150 let Abaya str., Nur- Sultan, 010000, Kazakhstan
M.A. Berdikulov
National Reference Center for Veterinary Medicine, 22/3, 150 let Abaya str., Nur- Sultan, 010000, Kazakhstan
R.N. Kuspekov
National Reference Center for Veterinary Medicine, 22/3, 150 let Abaya str., Nur- Sultan, 010000, Kazakhstan
A.G. Tegzhanov
National Reference Center for Veterinary Medicine, 22/3, 150 let Abaya str., Nur- Sultan, 010000, Kazakhstan
D.A. Tokaeva
National Reference Center for Veterinary Medicine, 22/3, 150 let Abaya str., Nur- Sultan, 010000, Kazakhstan
A.S. Tashkenbaev
National Reference Center for Veterinary Medicine, 22/3, 150 let Abaya str., Nur- Sultan, 010000, Kazakhstan
T.B. Birzhanov
National Reference Center for Veterinary Medicine, 22/3, 150 let Abaya str., Nur- Sultan, 010000, Kazakhstan
Zh.Zh. Aitzhanov
National Reference Center for Veterinary Medicine, 22/3, 150 let Abaya str., Nur- Sultan, 010000, Kazakhstan
N.M. Zhumakhmet
National Reference Center for Veterinary Medicine, 22/3, 150 let Abaya str., Nur- Sultan, 010000, Kazakhstan
N. Ispullaev
National Reference Center for Veterinary Medicine, 22/3, 150 let Abaya str., Nur- Sultan, 010000, Kazakhstan
T.E. Kozhakhmetova
National Reference Center for Veterinary Medicine, 22/3, 150 let Abaya str., Nur- Sultan, 010000, Kazakhstan
F.E. Suraganova
National Reference Center for Veterinary Medicine, 22/3, 150 let Abaya str., Nur- Sultan, 010000, Kazakhstan
Abstract
Anthrax is an infectious disease that affects many species of mammals and humans. Bacteriological isolation of the pathogen and its identification by phenotypic and PCR methods remains the gold standard. However, these methods do not allow determining the cause of the outbreak, namely, their origin from one or more sources. In addition, the causative agents of especially dangerous infections are deposited in the National Collections, and genotyping with the selection of a single representative strain is a prerequisite to prevent the loss of strains with unique properties, as well as the deposition of identical strains. In this study, we performed MLVA genotyping of 10 isolates from 5 outbreaks of anthrax in animals in 2021. The obtained MLVA profiles allowed differentiation of isolates in different outbreaks and confirmed the identity of genotypes in each outbreak. MLVA genotyping of B. anthracis can be recommended as the primary method for genotyping and selection of unique strains for replenishment of the collection.
Keywords
anthrax, isolate, MLVA, genotyping, B. anthracis, PCR
Article Details
References
Shandomy A.E., Raizman E., Bruni M., Palamara E., Pittiglio C., Lubroth J. Anthrax outbreaks: a warning for improved prevention, control and heightened awareness, 2016, pp. 37.
Goel A. K. Anthrax: A disease of biowarfare and public health importance. World Journal of Clinical Cases: WJCC., 2015, vol. 3(1), pp. 20.
Beyer W., and P.C.B. Turnbull. Anthrax in animals. Molecular aspects of medicine. 2009, vol. 30.6, pp. 481-489.
Hanczaruk M., Reischl U., Holzmann T., Frangoulidis D., Wagner D.M., Keim P.S., Antwerpen M.H., Meyer H., Grass G. Injectional anthrax in heroin users, Europe, 2000-2012. Emerg. Infect. Dis., 2014, vol. 20, no.2, pp. 322-323. doi:10.3201/eid2002.120921
Hugh-Jones M., Blackburn J. The ecology of Bacillus anthracis. Mol. Aspects. Med., 2009, vol. 30, no 6, pp. 356-367.
Kanankege K. S., Abdrakhmanov S. K., Alvarez J., Glaser L., Bender J. B., Mukhanbetkaliyev Y. Y., Perez A. M. Comparison of spatiotemporal patterns of historic natural Anthrax outbreaks in Minnesota and Kazakhstan. PloS one., 2019, vol.14(5), e0217144.
Aminu O. R., Lembo T., Zadoks R. N., Biek R., Lewis S., Kiwelu I., Forde T. L. Practical and effective diagnosis of animal anthrax in endemic low-resource settings. PLoS neglected tropical diseases., 2020, vol. 14(9), e0008655.
Van Ert M. N., Easterday W. R., Huynh L. Y., Okinaka R. T., Hugh-Jones M. E., Ravel J., Zanecki S. R., Pearson T., Simonson T. S., U'Ren J. M., Kachur S. M., Leadem-Dougherty R. R., Rhoton S. D., Zinser G., Farlow J., Coker P. R., Smith K. L., Wang B., Kenefic L. J., Fraser-Liggett C. M., Wagner D. M., Keim P. Global genetic population structure of Bacillus anthracis. PLoS One. 2007, vol. 2, no. 5. doi: 10.1371/journal.pone.0000461.
Keim P., Kalif A., Schupp J., Hill K., Travis S. E., Richmond K., Jackson P. Molecular evolution and diversity in Bacillus anthracis as detected by amplified fragment length polymorphism markers. Journal of bacteriology. 1997, vol. 179, no.3, pp. 818-824.
Keim P., Price L. B., Klevytska A. M., Smith K. L., Schupp J. M., Okinaka R., Jackson P. J., Hugh-Jones M. E. Multiple-locus variable-number tandem repeat analysis reveals genetic relationships within Bacillus anthracis // J .Bacteriol. – 2000. – Vol. 182. – № 10. – P.2928-2936.
Gierczynski R., Kałuzewski S., Rakin A., Jagielski M., Zasada A., Jakubczak A., Borkowska-Opacka B., Rastawicki W. Intriguing diversity of Bacillus anthracis in eastern Poland - the molecular echoes of the past outbreaks. FEMS Microbiol. Lett., 2004, vol. 239,no 2, pp. 235-240.
Durmaz R., Doganay M., Sahin M., Percin D., Karahocagil M. K., Kayabas U., Otlu B., Karagoz A., Buyuk F., Celebi O., Ozturk Z., Ertek M. Molecular epidemiology of the Bacillus anthracis isolates collected throughout Turkey from 1983 to 2011. Anthrax Study Group. Eur. J. Clin. Microbiol. Infect Dis., 2012, vol. 31, no 10, pp. 2783-2790.
Le Fleche P., Hauck Y., Onteniente L., Prieur A., Denoeud F., Ramisse V., Sylvestre P., Benson G., Ramisse F., Vergnaud G.A. Tandem repeats database for bacterial genomes: application to the genotyping of Yersinia pestis and Bacillus anthracis. BMC Microbiol., 2001, vol. 1, pp. 2.
Lista F., Faggioni G., Valjevac S., Ciammaruconi A., Vaissaire J. Genotyping of Bacillus anthracis strains based on automated capillary 25-loci multiple locus variable-number tandem repeats analysis. BMC Microbiol., 2006, vol. 6, pp. 33.
Rondinone V., Galante D., Donatiello A., Serrecchia L., Manzulli V., Cipolletta D., Fasanella A. Phylogenetic study of the Bacillus anthracis strains isolated from Italian outbreaks by analyzing Canonical SNPs and MLVA at 31 loci. Società Italiana di Diagnostica di Laboratorio Veterinaria. 2018, pp. 52-53.
Yu D., He J., Zhang E., Wang P., Liu D., Hou Y., Wei, J. Investigation and source-tracing of an anthrax outbreak in Gansu Province, China. PloS one. 2018, vol.13(8), e0203267.
Shevtsov A.B., Lutsay V.B., Kairzhanova A.D., Lukhnova L.Y., Kulatay T.Z., Izbanova U.A., Karibaev T.B., Shustov, A.V. Optimization of genotyping protocol for B. anthracis using multiple-locus VNTR analysis MLVA-31. Eurasian j. appl. biotechnol., 2019, no 2, pp. 103-113.
Beyer W., Bellan S., Eberle G., Ganz H.H., Getz W.M., Haumacher R., Hilss K.A., Kilian W., Lazak J., Turner W.C., Turnbull P.C. Distribution and molecular evolution of Bacillus anthracis genotypes in Namibia. PLoS Negl. Trop. Dis., 2012, vol. 6, e1534.
Thierry S., Tourterel C., Le Flèche P., Derzelle S., Dekhil N., Mendy C., Colaneri C., Vergnaud G., Madani N. Genotyping of French Bacillus anthracis Strains Based on 31-Loci Multi Locus VNTR Analysis: Epidemiology, Marker Evaluation, and Update of the Internet Genotype Database. PLoS One. 2014, vol. 9, e95131.
Muller J., Mohammad I., Warner S., Paskin R., Constable F., Fegan M. Genetic Diversity of Australian Bacillus anthracis Isolates Revealed by Multiple-Locus Variable-Number Tandem Repeat Analysis. Microorganisms. 2020, vol.8(6), pp.886.
Le Flèche P., Jacques I., Grayon M., Al Dahouk S., Bouchon P., Denoeud F., Nöckler K., Neubauer H., Guilloteau L.A., Vergnaud G. Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay. BMC Microbiology. 2006, vol.6:9. doi: 10.1186/1471-2180-6-9.
Antwerpen M., Ilin D., Georgieva E., Meyer H., Savov E., Frangoulidis D. MLVA and SNP analysis identified a unique genetic cluster in Bulgarian Bacillus anthracis strains. Eur. J. Clin. Microbiol. Infect. Dis., 2011, vol. 30, pp. 923-930. doi: 10.1007/s10096-011-1177-2.
Pullan S.T., Pearson T.R., Latham J., Mason J., Atkinson B., Silman N.J., Marston C.K., Sahl J.W., Birdsell D., Hoffmaster A.R., et al. Whole-genome sequencing investigation of animal-skin-drum-associated UK anthrax cases reveals evidence of mixed populations and relatedness to a US case. Microb. Genom., 2015, vol. 1, e000039. doi: 10.1099/mgen.0.000039.
Vergnaud G., Girault G., Thierry S., Pourcel C., Madani N., Blouin Y. Comparison of French and worldwide Bacillus anthracis strains favors a recent, post-Columbian origin of the predominant North-American clade. PLoS ONE, 2016, vol. 11, e0146216. doi: 10.1371/journal.pone.0146216.
Eremenko E., Pechkovskii G., Pisarenko S., Ryazanova A., Kovalev D., Semenova O. G., Kulichenko A. Phylogenetics of Bacillus anthracis isolates from Russia and bordering countries. Infection, Genetics and Evolution., 2021, vol. 92, e104890.
Simonson T. S., Okinaka R. T., Wang B., Easterday W. R., Huynh L., U'Ren J. M., Keim, P. Bacillus anthracis in China and its relationship to worldwide lineages. BMC Microbiology, 2009, vol. 9, no.1, pp. 1-11.