BIOLOGICAL PROPERTIES OF THE SARS-COV-2 VIRUS ISOLATED FROM A DOG ON THE TERRITORY OF THE REPUBLIC OF KAZAKHSTAN
Main Article Content
Authors
S.O. Sadikalieva
Research Institute for Biological Safety Problems, Guardeyskiy uts., Kazakhstan
E.A. Shayakhmetov
Research Institute for Biological Safety Problems, Guardeyskiy uts., Kazakhstan
Zh.S. Abay
Research Institute for Biological Safety Problems, Guardeyskiy uts., Kazakhstan
A.S. Nurpeisova
Research Institute for Biological Safety Problems, Guardeyskiy uts., Kazakhstan
K.A. Shoraуeva
Research Institute for Biological Safety Problems, Guardeyskiy uts., Kazakhstan
A.D. Omurtaу
Research Institute for Biological Safety Problems, Guardeyskiy uts., Kazakhstan
S.K. Kopeev
Research Institute for Biological Safety Problems, Guardeyskiy uts., Kazakhstan
A.K. Nakhanov
Research Institute for Biological Safety Problems, Guardeyskiy uts., Kazakhstan
A.A. Terebay
Research Institute for Biological Safety Problems, Guardeyskiy uts., Kazakhstan
B.Sh. Myrzakhmetova
Research Institute for Biological Safety Problems, Guardeyskiy uts., Kazakhstan
N.S. Kozhabergenov
Research Institute for Biological Safety Problems, Guardeyskiy uts., Kazakhstan
Ye.O. Abduraimov
Research Institute for Biological Safety Problems, Guardeyskiy uts., Kazakhstan
Abstract
This article examined biological samples (28 swabs) taken from dogs in the Almaty region of the Republic of Kazakhstan as part of monitoring studies of the SARS-COV-2 virus among domestic animals.
The resulting samples were analyzed by real-time RT-PCR, where seven (7) samples were detected within cycles 25-37. Further, these samples were cultured in Vero cell culture to determine the presence of the virus.
As a result of reproduction on a Vero cell culture, a viral suspension of one isolate was obtained with the biological activity of 5.83±0.08 lgTCID50/cm3. In addition, Electron microscopy confirmed virion morphology similar to that of the Coronaviridae family.
The data obtained indicate that pets can also get infected with COVID-19 coronavirus infection and be carriers of this disease.
Keywords
biological samples, SARS-COV-2 virus, PCR analysis, RNA, domestic animals, Vero cell culture, electron microscopy
Article Details
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