THE OBTAINING OF THE RECOMBINANT CAMEL CHYMOSIN BY SUBMERGE FERMENTATION IN THE PILOT BIOREACTOR
Main Article Content
Authors
Zh. Akishev
National Center for Biotechnology, 13/5 Korgalzhyn Road, Astana, 010000
Faculty of natural sciences, L.N.Gumilyev Eurasian National University, 2 Kanysh Satpayev Street, Astana, 010008
A. Abdullayeva
National Center for Biotechnology, 13/5 Korgalzhyn Road, Astana, 010000
A. Mussakhmetov
National Center for Biotechnology, 13/5 Korgalzhyn Road, Astana, 010000
Faculty of natural sciences, L.N.Gumilyev Eurasian National University, 2 Kanysh Satpayev Street, Astana, 010008
A. Bekbayeva
National Center for Biotechnology, 13/5 Korgalzhyn Road, Astana, 010000
Faculty of natural sciences, L.N.Gumilyev Eurasian National University, 2 Kanysh Satpayev Street, Astana, 010008
A. Tursunbekova
S.Seifullin Kazakh Agro Technical University, 62 Zhenis Avenue, Astana, 010001
B. Kalemshariv
S.Seifullin Kazakh Agro Technical University, 62 Zhenis Avenue, Astana, 010001
T. Tultabayeva
S.Seifullin Kazakh Agro Technical University, 62 Zhenis Avenue, Astana, 010001
B. Khassenov
National Center for Biotechnology, 13/5 Korgalzhyn Road, Astana, 010000
“GenLab” LLP, 19/1, 69, M. Gabdullin Street, Astana, 010000
Abstract
In the cheese making industry, chymosin is used as a milk-clotting enzyme. With its high specific activity against κ-casein, chymosin better than other proteolytic enzymes. Bactrian camel chymosin has a milk-clotting activity higher than calf chymosin. A scheme for obtaining a milk-clotting preparation based on recombinant camel chymosin is proposed. Submerge fermentation of recombinant yeast Pichia pastoris was carried out in a 50-liter bioreactor and recombinant camel chymosin was obtained. The activity of chymosin in the yeast culture was 174.5 U/mL. Chymosin was concentrated 5.6-fold by cross-flow ultrafiltration with 10 kDa cut-off membrane, and chymosin was purified by ion exchange chromatography. The activity of purified chymosin was 4700 U/mL. By sublimation drying with casein peptone, the powder chymosin was obtained with an activity of 36,000 U/g. The proposed scheme for obtaining a milk-clotting drug based on recombinant camel chymosin using submerge fermentation of recombinant yeast has the prospect of being used at biotechnological enterprises.
Keywords
Chymosin, Fermentation, Camel, Calf, Bioreactor, Freeze-drying
Article Details
References
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