THE INFLUENCE OF PHYSICAL FACTORS ON THE GROWTH OF BACTERIA-ANTAGONIST BACILLUS SUBTILIS BIM B-760 D
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Authors
V.N. Kuptsov
Institute of Microbiology, NAS of Belarus, Kuprevich Str. 2, 220141, Minsk, Belarus
E.Y. Shmyga, Institute of Microbiology of National Academy of Sciences of Belarus
Institute of Microbiology, NAS of Belarus, Kuprevich Str. 2, 220141, Minsk, Belarus
E.I. Kolomiets
Institute of Microbiology, NAS of Belarus, Kuprevich Str. 2, 220141, Minsk, Belarus
R.K. Nagorny
Institute of Physics, NAS of Belarus, Nezavisimosti Ave. 68-2, 220072, Minsk, Belarus
V.U. Plavsky
Institute of Physics, NAS of Belarus, Nezavisimosti Ave. 68-2, 220072, Minsk, Belarus
Abstract
The influence of electric current and electromagnetic radiation on the growth and antimicrobial activity of bacteria B. subtilis BIM B-760 D - the basis of the biopesticide "Bactaven C" was studied. It was determined that the use of inoculum treated with an alternating current of 16 mA and frequency 17 Hz has enabled to obtain a 2-day culture of B. subtilis BIM B-760 D with a titer of CFU and spores 2.4 – 2.7 times higher compared to the variant without electrical stimulation. Exposure of the seed material for 30 minutes in the electromagnetic field of a Tesla transformer (frequency 490 kHz) with a multi–needle electrode promoted 1.9 – 2.0 times increase in the titer of spores, CFU and 12 % higher antifungal activity by 48 h of culture.
Keywords
bacteria, fungi, CFU, spores, alternating current, electromagnetic radiation
Article Details
References
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