SCREENING OF GENES RESPONSIBLE FOR β-GLUCAN SYNTHESIS IN GRAIN VARIETIES
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Authors
G.A. Ismagulova
M.A. Aitkhozhin’s institute of molecular biоlogy and biochemistry CS MES RK, Dosmukhamedov st., 86, Almaty, 050012, Kazakhstan
S.S. Baizhumanova
M.A. Aitkhozhin’s institute of molecular biоlogy and biochemistry CS MES RK, Dosmukhamedov st., 86, Almaty, 050012, Kazakhstan
G.A. Iskakova
M.A. Aitkhozhin’s institute of molecular biоlogy and biochemistry CS MES RK, Dosmukhamedov st., 86, Almaty, 050012, Kazakhstan
D.O. Baisaparova
M.A. Aitkhozhin’s institute of molecular biоlogy and biochemistry CS MES RK, Dosmukhamedov st., 86, Almaty, 050012, Kazakhstan
A.M. Mendesh
M.A. Aitkhozhin’s institute of molecular biоlogy and biochemistry CS MES RK, Dosmukhamedov st., 86, Almaty, 050012, Kazakhstan
A.A. Tussipova
Al-Farabi Kazakh National University, Al-Farabi av. 71, 050040 Almaty
Yu.A. Skiba
M.A. Aitkhozhin’s institute of molecular biоlogy and biochemistry CS MES RK, Dosmukhamedov st., 86, Almaty, 050012, Kazakhstan
Almaty Branch of the National Center for Biotechnology, Zhahanger st. 14, Almaty, 050054, Kazakhstan
E.R. Maltseva
M.A. Aitkhozhin’s institute of molecular biоlogy and biochemistry CS MES RK, Dosmukhamedov st., 86, Almaty, 050012, Kazakhstan
Almaty Branch of the National Center for Biotechnology, Zhahanger st. 14, Almaty, 050054, Kazakhstan
D.A. Naizabayeva
M.A. Aitkhozhin’s institute of molecular biоlogy and biochemistry CS MES RK, Dosmukhamedov st., 86, Almaty, 050012, Kazakhstan
Almaty Branch of the National Center for Biotechnology, Zhahanger st. 14, Almaty, 050054, Kazakhstan
M.A. Esimbekova
LLP “KazRIAPG”, Almalybak village, Almaty region, 040909, Kazakhstan
K.B. Mukin
LLP “KazRIAPG”, Almalybak village, Almaty region, 040909, Kazakhstan
Abstract
Currently, β-glucan has a great importance for human life. Because it reduces the risks of many chronic diseases such as diabetes, cancer and digestive disorders. Due to these properties, β-glucan is a natural substance that can be used in pharmaceutical preparations for the prevention and treatment of many chronic diseases. Therefore, an analysis was made for crops rich in sources of β-glucans - 100 varieties of wheat, 50 varieties of barley and 50 varieties of oats, and a collection of genomic DNA was made. Each variety was screened to identify the genes responsible for the synthesis of β-glucans. In the frames of this study, 10 genes were considered. As a result, these genes have been identified in barley, wheat, and oat varieties.
For the isolation of β-glucan, the methods of acid extraction, microwave, and heating at high temperature were used. A modified method for isolating β-glucan from oatmeal was developed. According to the results, the acid extraction method proved to be more effective. It is easy to obtain pure β-glucan by this method, and the use of citric acid is safer and more affordable than conventional acids. The improved acid extraction method showed that the concentration of pure β-glucan per 10 g of wheat grain was 66.6%, and the total yield of β-glucan extract increased to 11.3%.
Keywords
β-glucan, genes, wheat, barley, oats, screening, extraction
Article Details
References
Bangari, S. Effects of Oat Beta Glucan on the Stability and Textural Properties of Beta Glucan Fortified Milk Beverage. A Research Paper Submitted in Partial Fulfillment of the Requirements for the Master of Science Degree III Food and Nutritional Sciences. University of Wisconsin-Stout, Menomonie, WI, 2011. 51 p.
Havrlentová, M., Petruláková, Z., Burgárová, A., Gago, F., Hlinková, A. and Šturdík, E. Cereal β-glucans and their Significance for the Preparation of Functional Foods. // Czech J. Food Sci. - 2011. - Vol. 29. - P. 1-14.
Katongole, J.N. Aqueous protein based extraction of oat beta glucan and its physiological effects on satiety and glycaemic responses in healthy adults. // A Thesis the degree of Master of Science In Food Science. The University of Guelph, Ontario, Canada, 2011- 119 p.
Christopher, H. Beta Glucan a 21st Century Miracle: U.S.A. Published by www.booksmango.com. 2013.
Bell, S. J., Armour Forse, R., Bistrian, B. R. // Dietary supplement and method for lowering risk of heart disease. US Patent – 2001. – N. 6. – P.210-217.
Keogh, G.F., Cooper, G.J., Mulvey, T.B., McArdle, B.H., Coles, G.D., Monro, J.A., Poppitt, S.D. Randomized controlled crossover study of the effect of a highly beta-glucan-enriched barley on cardiovascular disease risk factors in mildly hypercholesterolemic men // Am J Clin Nutr. – 2003. – N.78. – P. 711-718.
Nicolosi, R., Bell, S. J., Bistrian, B. R., Greenberg, I., Forse, R. A., Blackburn, G. L. Plasma lipid changes aft er supplementations with β-glucan fiber from yeast // Am J Clin Nutr. – 1999. –N. 70. – P. 208-212.
Steriti, R. Beta-glucans for cardiovascular support // Dynamic Chiropractis. – 2007. – N. 7. – P. 1-6.
Todd, A. Richmond, Chris R. Somerville. The Cellulose Synthase Superfamily 1. // Plant Physiology. – 2000. - Vol. 124. - P. 495–498. Crossref.
[Schwerdt, J.G., MacKenzie, K., Wright, F., Oehme, D., Wagner, J.M., Harvey, A.J., Shirley, N.J., Burton, R.A., Schreiber, M., Halpin, C. Evolutionary dynamics of the cellulose synthase gene superfamily in grasses.// Plant Physiol. – 2015. – Vol.168(3). – P. 968–83.
Simerjeet Kaur, Kanwarpal S. Dhugga, Robin Beech and Jaswinder Singh. Genome-wide analysis of the cellulose synthase-like (Csl) gene family in bread wheat (Triticum aestivum L.). // BMC Plant Biology. – 2017. - 17:193. DOI 10.1186/s12870-017-1142-z.
Hazen, S.P., Scott-Craig, J.S., and Walton J.D. Cellulose Synthase-Like Genes of Rice. // Plant Physiology. – 2002. - Vol. 128. - P. 336–340.
Genomic DNA Purification Kit — [Электронный ресурс] — Режим доступа. — URL:URL genomic-dna-purification-kit/ (дата обращения 21.04.2014).
Bin, Du, Fengmwi, Zhu, Baojun, Xu. β-Glucan extraction from bran of hull-less barley by accelerated solvent extraction combined with response surface methodology. // Journal of Cereal Science. – 2014. – N. 59. – P. 95-100.
Ahmada, A., Anjum, F.M., Zahoorb, T., Nawazc, H. and Ahmed, Z. Extraction and characterization of β-D-glucan from oat for industrial utilization. // International Journal of Biological Macromolecules. - 2010. - Vol. 46. - P. 304–309.
Wood, P.J., Paton, D. and Siddiqui, I.R. Determination of β-Glucan in oats and barley. // Cereal Chem. - 1976. - Vol. 54. - P. 524-533.
Limberger-Bayer, V.M., Francisco, A.D., Chan, A., Oro, T., Ogliari, P.J. and Barreto, P.L.M. Barley β-glucans extraction and partial characterization. // Food Chemistry. - 2014. - Vol. 154. - P. 84-89.
Takuji Tonooka, Emiko Aoki, Toji Yoshioka and Shin Taketa. A novel mutant gene for (1-3, 1-4)-β-D-glucanless grain on barley (Hordeum vulgare L.) chromosome 7H. // Breeding Science. – 2009. - Vol. 59. – Is.1. – P. 47-54. Crossref