THE USE OF MOLECULAR GENETIC METHODS BASED ON MLVA ANALYSIS TO CONFIRM THE UNIQUENESS OF COLLECTION STRAINS OF BRUCELLA
Main Article Content
Authors
K. Berdimuratova
National center for biotechnology, 13/5, Kurgalzhynskoye road, Nur-Sultan, 010000, Kazakhstan
E. Shevtsova
National center for biotechnology, 13/5, Kurgalzhynskoye road, Nur-Sultan, 010000, Kazakhstan
A. Kairzhanova
National center for biotechnology, 13/5, Kurgalzhynskoye road, Nur-Sultan, 010000, Kazakhstan
B. Abdigulov
National center for biotechnology, 13/5, Kurgalzhynskoye road, Nur-Sultan, 010000, Kazakhstan
T. Karibayev
National Reference Center Veterinary, street 150 let Abaya, house 22/3, Nur-Sultan 010000, Kazakhstan
M. Berdikulov
National Reference Center Veterinary, street 150 let Abaya, house 22/3, Nur-Sultan 010000, Kazakhstan
S. Firsova
National Reference Center Veterinary, street 150 let Abaya, house 22/3, Nur-Sultan 010000, Kazakhstan
A. Shevtsov
National center for biotechnology, 13/5, Kurgalzhynskoye road, Nur-Sultan, 010000, Kazakhstan
Abstract
Collections of pathogenic bacterial cultures are necessary for the storage of commercially valuable and typical strains for the purpose of patenting, production and testing of diagnostic and preventive drugs, as well as epidemiological strains isolated during the diagnosis of infectious diseases for detailed study and monitoring of changes in sanitary and epidemiological well-being. Collections are replenished with new unique strains, so it is very important that the characteristics of these strains should be carried out using highly discriminatory molecular genetic methods. This article presents the results of the evaluation of the genetic diversity of 36 collection strains of Brucella abortus and 57 strains of Brucella melitensis by the Multilocus variable number tandem repeat (VNTR) analysis (MLVA-16). Generic identification and absence of contamination by other bacterial species was confirmed by sequencing of the 16S rRNA gene. MLVA analysis allowed to establish contamination of 4 strains with different genotypes of brucella and to reveal that the deposited 36 strains isolated in 14 outbreaks of brucellosis are identical genotypes of the pathogen. Our studies have confirmed the presence of identical brucella strains in the collection. The use of MLVA-16 in testing isolated bacterial cultures from a single outbreak will allow differentiating unique strains for their subsequent deposition. Verification of deposited strains with MLVA characteristics with a detailed analysis of metadata about deposited strains will optimize the collection, in terms of preserving only unique strains, which will reduce economic costs and risks to ensure biological safety.
Keywords
Brucella melitensis, Brucella abortus, genotyping, MLVA, Kazakhstan
Article Details
References
Bell T., Newman J. A., Silverman B. W., Turner S. L., Lilley A. K. // The contribution of species richness and composition to bacterial services. Nature. -2005. - №436 (7054). - Р.1157-1160.
Wagg C., Bender S. F., Widmer F., Van Der Heijden M. G. // Soil biodiversity and soil community composition determine ecosystem multifunctionality. Proceedings of the National Academy of Sciences. -2014. -№111(14). – Р. 5266-5270.
Walters K. E., Martiny J. B. // Alpha-, beta-, and gamma-diversity of bacteria varies across habitats. PLoS One. - 2020. - №15(9), doi.org/10.1371/journal.pone.0233872
Soetaert W., Vandamme E. J. // The scope and impact of industrial biotechnology. Industrial biotechnology: sustainable growth and economic success. – 2010. – Р.1-16.
Malik K. A., Claus D. // Bacterial culture collections: their importance to biotechnology and microbiology. Biotechnology and Genetic engineering reviews. -1987. - №5(1). – Р.137-198.
Barba M., Van den Bergh I., Belisario A., Beed F. // The need for culture collections to support plant pathogen diagnostic networks. Research in microbiology. – 2010. - №161(6). – Р. 472-479.
Oslan S. N. H., Tan J. S., Yusoff A. H., Sulaiman A. Z., Awang M. A., Lazim A. M., Ariff A. B. // Pasteurellosis Vaccine Commercialization: Physiochemical Factors for Optimum Production. Processes. - 2022. - №10(7). – Р.1248.
Peeling R. W., Smith P. G., Bossuyt P. M. // A guide for diagnostic evaluations. Nature Reviews Microbiology. – 2006. - №4(9). – Р. 2-6.
Onishchenko, G. G., Kutyrev, V. V., Osin, A. V. // Kollekcionnaya deyatelnost v oblasti ispolzovaniya patogennyh mikroorganizmov v obespecshenii biologicsheskoi bezopasnosti. Rossiskoi Federacii. Infekcionnye bolezni. Novosti. Mneniya. Obuschenie – 2016. - №1 (14). – Р. 37-46.
Clark W. A., Loegering W. Q. // Functions and maintenance of a type-culture collection. Annual Review of Phytopathology. -1967. - № 5(1). – Р. 319-342.
Caktu K., Turkoglu, E. // Microbial culture collections: The essential resources for life. Gazi University Journal of Science. – 2011. - № 24(2). – Р.175-180.
Tang P., Croxen M. A., Hasan M. R., Hsiao W. W., Hoang L. M. // Infection control in the new age of genomic epidemiology. American Journal of Infection Control. – 2017. - № 45(2). – Р. 170-179.
Shevtsov V., Kairzhanova A., Shevtsov A., Shustov A., Kalendar R., Abdrakhmanov, S., Vergnaud G. // Genetic diversity of Francisella tularensis subsp. holarctica in Kazakhstan. PLoS Neglected Tropical Diseases. -2021. - №15(5), doi.org/10.1371/journal.pntd.0009419
Sanchez-Cespedes J., Figueras M. J., Aspiroz C., Aldea M. J., Toledo M., Alperi A., Vila J. // Development of imipenem resistance in an Aeromonas veronii biovar sobria clinical isolate recovered from a patient with cholangitis. Journal of medical microbiology. – 2009. - № 58(4). - Р. 451-455.
Le Flèche P., Jacques I., Grayon M., Al Dahouk S., Bouchon P., Denoeud F. // Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay. BMC Microbiol. - 2006. - №9, doi: 10.1186/1471-2180-6-9
Al Dahouk S., Le Flèche P., Nockler K., Jacques I., Grayon M., Scholz H. C. // Evaluation of Brucella MLVA typing for human brucellosis. J. Microbiol. – 2007. - №69. – Р.137–145, doi: 10.1016/j.mimet.2006.12.015
Garofolo G., Ancora M., Di Giannatale E. / MLVA-16 loci panel on Brucella spp. using multiplex PCR and multicolor capillary electrophoresis. J. Microbiol. - 2013. - № 92.-Р.103–107, doi: 10.1016/j.mimet.2012.11.007
Shevtsov A., Ramanculov E., Shevtsova E., Kairzhanova A., Tarlykov P., Filipenko M. //. Genetic diversity of Brucella abortus and Brucella melitensis in Kazakhstan using MLVA-16. Infect. Genet. Evol. - 2015. - №34.-Р. 173–180, doi: 10.1016/j.meegid.2015.07.008
Smith D. // Culture collections over the world. International Microbiology. - 2003. -№ 6(2). – Р. 95-100.
Thierry S., Tourterel C., Le Flèche P., Derzelle S., Dekhil N., Mendy C., Madani N. // Genotyping of French Bacillus anthracis strains based on 31-loci multi locus VNTR analysis: epidemiology, marker evaluation, and update of the internet genotype database. PLoS One. -2014. №9(6), doi: 10.1371/journal.pone.0095131
Tanveer M., Hasan Z., Siddiqui A. R., Ali A., Kanji A., Ghebremicheal S., Hasan R. // Genotyping and drug resistance patterns of M. tuberculosis strains in Pakistan. BMC infectious diseases. -2008. - №8(1). – Р. 1-10.
Al Dahouk S., Le Flèche, P., Nöckler K., Jacques I., Grayon M., Scholz H. C., Neubauer, H.// Evaluation of Brucella MLVA typing for human brucellosis. Journal of microbiological methods. -2007. - №69(1). – Р.137-145.
Jiang H., Wang H., Xu L., Hu G., Ma J., Xiao P., Cui, B. // MLVA genotyping of Brucella melitensis and Brucella abortus isolates from different animal species and humans and identification of Brucella suis vaccine strain S2 from cattle in China. PLoS One. 2013. - №8(10), doi: 10.1371/journal.pone.0076332
Struelens M. J., Brisse S. // From molecular to genomic epidemiology: transforming surveillance and control of infectious diseases. Eurosurveillance. - 2013. - № 18(4). – Р. 20386.
Daugaliyeva А., Sultanov A., Usserbayev B., Baramova S., Modesto P., Adambayeva A., Peletto S. // Genotyping of Brucella melitensis and Brucella abortus strains in Kazakhstan using MLVA-15. Infection, Genetics and Evolution. -2018. -№58. –Р. 135-144.
Fu S., Octavia S., Wang Q., Tanaka M. M., Tay C. Y., Sintchenko V., Lan R. // (2016). Evolution of variable number tandem repeats and its relationship with genomic diversity in Salmonella Typhimurium. Frontiers in microbiology. – 2016. - №7. – Р. 2002.
Dorneles E. M. S., de Faria A. P. P., Pauletti R. B., Santana J. A., Caldeira G. A. V., Heinemann M. B., Lage A. P. // Genetic stability of Brucella abortus S19 and RB51 vaccine strains by multiple locus variable number tandem repeat analysis (MLVA16). Vaccine. – 2013. - №31(42). – Р. 4856-4859.