DNA barcoding of rare and endangered plant species of the Republic of Kazakhstan

Main Article Content

Authors

A. Sarsen

National center for biotechnology 13/5, Kurgalzhyn road, Nur-Sultan, 010000, Kazakhstan

M. Saginova

National center for biotechnology 13/5, Kurgalzhyn road, Nur-Sultan, 010000, Kazakhstan

S. Aktayeva

National center for biotechnology 13/5, Kurgalzhyn road, Nur-Sultan, 010000, Kazakhstan

Zh. Akishev

National center for biotechnology 13/5, Kurgalzhyn road, Nur-Sultan, 010000, Kazakhstan

A. Kiribayeva

National center for biotechnology 13/5, Kurgalzhyn road, Nur-Sultan, 010000, Kazakhstan

Sh. Manabayeva

National center for biotechnology 13/5, Kurgalzhyn road, Nur-Sultan, 010000, Kazakhstan

A. Mussakhmetov

National center for biotechnology 13/5, Kurgalzhyn road, Nur-Sultan, 010000, Kazakhstan

A. Kakimzhanova

National center for biotechnology 13/5, Kurgalzhyn road, Nur-Sultan, 010000, Kazakhstan

O. Khapilina

National center for biotechnology 13/5, Kurgalzhyn road, Nur-Sultan, 010000, Kazakhstan

I. Akhmetollayev

National center for biotechnology 13/5, Kurgalzhyn road, Nur-Sultan, 010000, Kazakhstan

B. Khassenov

National center for biotechnology 13/5, Kurgalzhyn road, Nur-Sultan, 010000, Kazakhstan

Abstract

The ability to identify vegetation types is very important for the study of flora biodiversity. DNA barcoding makes it possible to standardize the identification procedure by reference DNA regions. The most popular sequences for molecular identification and phylogenetic studies are Internal Transcribed Spacer (ITS) sequences. ITS are widely used as a phylogenetic marker to classify plants at various taxonomic levels, including genus, species, and subspecies.            29 samples of rare and endangered plant species were collected from Aksu-Zhabagly State Nature Reserve, Sayram-Ugam National Park and Berikkara Tract State Nature Reserve. DNA was isolated by the CTAB method, oligonucleotides flanking the ITS region of the nuclear DNA region were selected and synthesized. Sequencing was carried out and ITS sequences of the region were obtained for 29 species of rare and endangered plant species in Kazakhstan.

Keywords

Flora, ITS, fauna, DNA barcoding, identification, СТАВ method

Article Details

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