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V.К. Каrimova

National Center for biotechnology, 13/1 Valikhanova str., Astana, 010000, Kazakhstan

G.К. Magzumova

National Center for biotechnology, 13/1 Valikhanova str., Astana, 010000, Kazakhstan

А.А. Кakimzhanova

National Center for biotechnology, 13/1 Valikhanova str., Astana, 010000, Kazakhstan


In vitro studies of Populus alba L. and Populus bolleana L. poplars were performed to optimize the mode of sterilization of explants, determine the composition of the culture medium for the cultivation of Murashige and Skoog (MS) axillary buds, and select the ideal temperatures for the microshoots.

In order to produce in vitro cultures of the bosom buds of male Populus alba L. and Populus bolleana L. poplars, a sterilization method (Tween-20 and 90% Belizna solution treatment for 20 min) of explants of the two poplar species was selected. Of the 679 axillary buds of poplar and silver Bolle that were cultivated on MS nutrient medium, the number of viable explants was 488 (71.9%).

For the cultivation of the axillary buds of Populus alba L. and Populus bolleana L., a cultivation medium consisting of MS medium and the plant phytohormones kinetin (0.25 mg/L) and indole acetic acid (0.25 mg/L) was used.

For the in vitro cultivation of microshoots, a temperature regimen was selected. The comparison of three temperatures (8°C, 22°C, and 28°C) showed active growth of the bosom buds of the two poplar species at 22°C.


Populus alba L., Populus bolleana L., bosom buds, cultivation medium, in vitro

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Zhumagulov J. Ozelenenie naselennykh punktov: sostoyanie, problemy sozdaniya i soderzhaniya zelenykh nasazhdeniy [Greening settlements: status, problems establishing and maintaining green spaces]. Available at: URL. 2011.

Noel N., Leple J.C., Pilate G. Optimization of in vitro micropropagation and regeneration for Populus × interamericana and Populus × euramericana hybrids (P. deltoides, P. trichocarpa, and P. nigra). Plant Cell Reports, 2002, vol. 20, pp. 1150-1155. http: doi: 10.1007.

Salah K. Effect of Different Media and Growth Regulators on the in vitro Shoot Proliferation of Aspen, Hybrid Aspen and White Poplar Male Tree and Molecular Analysis of Variants in Micropropagated Plants. Life Science Journal, 2011, vol. 8, pp. 177-184.

Erst A.A., Bakulin V.T. Klonalnoe mikrorazmnozhenie topolya sibirskogo serebristogo [Clonal micropropagation of poplar of Siberian silvery]. Turczaninowia, 2012, no. 15(1), pp. 58-62.

Haapala T., Pakkanen A., Pulkkinen P. Variation in survival and growth of cuttings in two clonal propagation methods for hybrid aspen (Populus tremula × P. tremuloides)/ Forest Ecol. and Management, 2004, vol. 193, no. 3, pp. 345-354. http: doi: 10.1016.

Maheshwari P., Kovalchuk I. Efficient shoot regeneration from intermodal explants of Populus angustifolia, Populus balsamifera and Populus deltoids. New Biotech, 2011, vol. 28, no. 6, pp. 778-787. http: doi: 10.1016.

Lebedev V.G., Bulatova I.V., Shadrina T.E. Primenenie metodov biotekhnologii dlya povyshenie produktivnosti lesnykh kultur [Shadrina t.e. and other Application of methods of biotechnology for the increase of the productivity of forest cultures of]. Lesohozjajstvennaja informacija - Forestry information, 2008, no. 3, pp. 28-29.

Ahuja M.R. Somatic cell differentiation and rapid clonal propagation of aspen. Silvae Genetica, 1983, vol. 3, no. 3, pp. 131-135.

Ahuja M.R. In vitro propagation of poplar and aspen. Cell and tissue culture in forestry, 1987, vol. 3, pp. 207-223.

Dirr M.A., Heuser C.W. The reference manual of woody plant propagation: from seed to tissue culture. Varsity Press, Athens, GA, 1987, 239 p. http: doi: 689004.

Frohlich H.J., Weisgerber H. Research on in vitro techniques within the framework of poplar breeding – results and future trends. Silvae Genetica, 1985, vol. 34, pp. 134-137. http: doi: 19860608327.

Kalinin F.L., Kushnir G.P., Sarnackaya V.V. Tekhologiya mikroklonalnogo razmnozheniya rasteniy [Technology of micropropagation and reproduction of plants]. K.: Publishing House Naukova Dumka, 1992, 232 p.

Kalashnikova E.A., Rodin A.R. Poluchenie posadochnogo materiala drevesnykh, thevetochnykh i travyanistykh rasteniy s ispolzovaniem metodov kletochnoy I gennoy inzheneriy [Production of planting-stock of arboreal, floral and grassy plants with the use of methods of the cellular and gene engineering]. M.: Textbook, 2001, 122 p.

Cavusoglu A., Ipekci-Altas Z., Bajrovic K., Gozukirmizi N. and Zehir A. Direct and indirect plant regeneration from various explants of eastern cottonwood clones (Populus deltoides Bartram ex Marsh.) with tissue culture. African Journal of Biotechnology, 2011, vol. 10(16), pp. 3216-3221. http: doi: 10.5897/AJB10.2400.

Kaňuchová A., Ďurkovič J. Wood ontogeny during ex vitro acclimatization in micropropagated hybrid poplar clones. Biologia plantarum, 2013, vol. 57(1), pp. 144-148. http: doi: 10.1007/s10535-012-0122-2.

Kalcsits L., Silim Ж., Tanino Ж. Warm temperature accelerates short photoperiod-induced growth cessation and dormancy induction in hybrid poplar (Populus x spp.). Trees, 2009, vol. 23, pp. 971–979. http: doi: 10.1007/s00468-009-0339-7.