EFFECTS OF EXPOSURE TO MICROWAVE RADIATION AND NARROWBAND LASER ON THE REGENERATION OF MERISTEMATIC EXPLANTS OF POTATO
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Authors
T.K. Bekseitov
Pavlodar State University named after S. Toraigyrov, 64 Lomov str., Pavlodar, 140008, Kazakhstan
I.N. Anikinа
Pavlodar State University named after S. Toraigyrov, 64 Lomov str., Pavlodar, 140008, Kazakhstan
G.G. Dzhaksybaeva
Pavlodar State University named after S. Toraigyrov, 64 Lomov str., Pavlodar, 140008, Kazakhstan
D.D. Seytzhanova
Pavlodar State University named after S. Toraigyrov, 64 Lomov str., Pavlodar, 140008, Kazakhstan
Abstract
Virus-free seed potato are used worldwide as meristems. The limitation in the implementation of this method in practice is the very small size of the virus-free zone of the meristem, which is often located within 0.05 mm, and meristematic explants of such sizes are characterized by a weak capacity for regeneration that is considerably difficult to obtain in first tube-plants. For efficiency, apical meristems are used as chemical stimulating factors, and physical factors increase the regenerative capacity of meristems. In this study, we first investigated the possibility of the use of potato meristems complex explants for the stimulation of the regenerative capacity with microwave irradiation-selected rays and narrowband lasers. For irradiation, a high-frequency signal generator with a frequency range of 37.5–53.57 GHz. The microwave power was 20 mW.
As a result, the following data were obtained: irradiation with microwave beams and narrowband lasers for 60 min and 80 min on medium meristems promoted the accelerated passage of the phases of development, increased size characteristics, reduced death due to tissue necrosis, and thus increased their regenerative capacity. This method significantly increased the effectiveness of the technology of the source of virus-free seed potatoes.
Keywords
meristem, irradiation, cultivation, regenerant, explant, survival rate
Article Details
References
Babaev S.A., Aitbaev T.E., Alimgazinova B.Sh., Shvidchenko V.K., Hasanov V.T. Sozdanie banka zdorovyh sortov – osnova proizvodstva vysokokachestvennogo semennogo materiala kartofelja [Creation of a healthy varieties – the basis of the production of high-quality seed potatoes]. Vestnik selskohozjajstvennoj nauki Kazahstana- Bulletin of Agricultural Science of Kazakhstan, Almaty, Bastau Publ., 2008, vol. 3, pp. 17-18.
Atabekov I.G., Talyansky M.E. Biotehnologicheskie metody v bezvirusnom rastenievodstve [Biotechnological methods in disease-free plant]. Moscow, Nauka Publ., 2008, pp. 28-30.
Shmyglya V.A., Kinyakin N.F., Kutsamanova I. Priemy ozdorovlenija kartofelja ot virusnyh boleznej [Methods of improvement of potato virus diseases]. Moscow, 2009, pp. 133-145.
Blotskaya Z.V. Virusy kartofelja [Potato viruses]. Minsk, Vintage Publ., 2008, 33 р.
Muromtsev G.S., Butenko R.G. e.a. Основы сельскохозяйственной биотехнологии [Fundamentals of Agricultural Biotechnology]. Moscow, 1990, 225 p.
Abdildaev V.S. Bezvirusnoe semenovodstvo kartofelja v uslovijah Jugo-Vostoka Kazahstana [Disease-free seed potatoes in a South-East Kazakhstan]. Avtoref. Doct. Diss. Almaty, 2004, 48 p.
Zhumageldinova J.A. Vyrashhivanie kartofelja, ozdorovlennogo metodom apikalnyh meristem v uslovijah Jugo-Vostoka Kazahstana [Growing potatoes, rehabilitation by apical meristems in a South-East Kazakhstan]. Avtoref. Doct. Diss. Almaty, 2002, 17 p.
Verderevsky T.D., Marinescu V.G. Virusnye i mikroplazmennye zabolevanija plodovyh kultur i vinograda [Virus and microplasma diseases of fruit crops and grapes]. Chisinau, Shtiintsa Publ., 1985, 276 p.
Diba A.N., Winkler G.N., Butenko R.G. Vlijanie nekotoryh stimuljatorov na vyhod zdorovogo posadochnogo materiala kartofelja, poluchennogo metodom kul'tury meristemy [Influence of some stimulants to the output of a healthy potato planting material produced by meristem culture]. Fiziologija rastenij - Plant physiology. Moscow, 1973, рр. 1291-1294.
Hamburg K.Z., Gamanets L.V. Effect of adenine to education of starch in plant tissue cultures. Reports of USSR, 1977, рр. 237-244.
Muromtsev G.S., Korenev V.M., Gerasimova N.M. Gibberellins and plant growth. Proc. "Plant growth regulators and natural. Moscow, Nauka Publ., 1977, рр. 193-216.
Diba A., Hayrapetyan E., Winkler G., Butenko R. Osveshhenie i morfogenez izolirovannyh meristem. [Kartofel i ovoshhi.Lighting and morphogenesis isolated meristems. Potatoes and vegetables]. Moscow, 1972, рр. 37-44.
Degtyarev A.A. Vlijanie rezonansnoj fotostimuljacii na urozhaj kartofelja v uslovijah predgornoj zony Vostochnogo Kazahstana [Effect of resonant photostimulation on the potato crop in the conditions of a foothill zone of Eastern Kazakhstan]. Sbornik nauchnyh trudov Vostochno-Kazahstanskoj gosudarstvennoj selskohozjajstvennoj opytnoj stancii, 1980, рр. 48-53.
Karavayeva N.P. Influence of pre-irradiation svetoimpulsnogo tubers on the productivity of potato. Proceedings Komifil. AN USSR, 1980, vol. 47, pp. 119-128.
Patent 2120739 Russian Federation A01N4 IPC/00. A method of regenerating meristems / Doroshenko N.P., Luzgin G.V., Karpov A.F.; applicant and patentee State Research Institute of Viticulture and Winemaking them. Ya.I. Potapenko. no. 2003127645/12; zayavl. 11.09.2003; publ. 10.12.05, Byul. no. 34, p. 9.