TRANSCRIPTIONAL RESPONSE OF THE MULTIDRUG RESISTANT Staphylococcus aureus FOLLOWING FS-1 EXPOSURE
Main Article Content
Authors
I.S. Korotetskiy
Scientific Center for Anti-infectious Drugs, 75V al-Farabi Ave., Almaty, 050060, Kazakhstan
S.V. Shilov
Scientific Center for Anti-infectious Drugs, 75V al-Farabi Ave., Almaty, 050060, Kazakhstan
S.V. Shvidko
Scientific Center for Anti-infectious Drugs, 75V al-Farabi Ave., Almaty, 050060, Kazakhstan
A.B. Jumagaziyeva
Scientific Center for Anti-infectious Drugs, 75V al-Farabi Ave., Almaty, 050060, Kazakhstan
N.A. Suldina
Scientific Center for Anti-infectious Drugs, 75V al-Farabi Ave., Almaty, 050060, Kazakhstan
N.V. Korotetskaya
Scientific Center for Anti-infectious Drugs, 75V al-Farabi Ave., Almaty, 050060, Kazakhstan
A.I. Ilin
Scientific Center for Anti-infectious Drugs, 75V al-Farabi Ave., Almaty, 050060, Kazakhstan
O.N. Reva
Department of Biochemistry, Centre for Bioinformatics and Computational Biology, University of Pretoria, Pretoria, South Africa
Abstract
The aim of this study was to evaluate transcriptional regulation in a model multidrug resistant Staphylococcus aureus (MRSA) culture, S. aureus ATCC® BAA-39™, after exposure to a new antibacterial drug, FS-1. Growth media were supplemented with FS-1 at the end of the lag phase and in the middle of the exponential growth phase. To investigate the immediate effects of the iodine-containing antibacterial complex FS-1, 24 RNA samples were obtained 5 min after exposure to the drug.RNA sequencing revealed substantial changes in gene expression in the lag phase and, to a lesser degree, in the exponential growth phase. Analysis of the regulated genes suggested that FS-1 primarily targeted microbial cell wall proteins. The effect of FS-1 on S. aureus gene expression was similar, but not identical, to differential gene regulation profiles caused by several cell wall-disrupting antibiotics including bacitracin, D-cycloserine, and oxacillin. However, the gene regulation profiles were not completely congruent, suggesting that FS-1 displays a significant level of antimicrobial activity specificity. Therefore, it is possible that combining FS-1 with other anti-staphylococcal antibiotics will result in a cumulative synergetic effect.
Keywords
S. aureus, antibiotic resistance, FS-1, RNA, gene expression
Article Details
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