OBTAINING OF AN EXPERIMENTAL RETINOPATHY MODEL IN RABBITS USING REPEATED INTRAVITREAL ADMINISTRATION OF VEGF AND BFGF
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Authors
A. Issabekova
Stem Cell Laboratory, National Center for Biotechnology, Astana, 010000, Kazakhstan
M. Sarsenova
Stem Cell Laboratory, National Center for Biotechnology, Astana, 010000, Kazakhstan
A. Dairov
Stem Cell Laboratory, National Center for Biotechnology, Astana, 010000, Kazakhstan
Institute of Natural Sciences, Department of General Biology and Genomics, L.N. Gumilyov Eurasian National University, Astana, 010008, Kazakhstan
M. Shakhatbayev
Stem Cell Laboratory, National Center for Biotechnology, Astana, 010000, Kazakhstan
Ch. Assylbayev
Stem Cell Laboratory, National Center for Biotechnology, Astana, 010000, Kazakhstan
M. Baidarbekov
National Scientific Center of Traumatology and Orthopedics Named After Academician N.D. Batpenov, Astana, 010000, Kazakhstan
E. Kadralieva
Department of Ophthalmology, Astana Medical University, Astana, 010000, Kazakhstan
Zh. Urazayeva
Astramed Vision Restoration Center, Astana, 010000, Kazakhstan
V. Ogay
Stem Cell Laboratory, National Center for Biotechnology, Astana, 010000, Kazakhstan
Abstract
Retinopathy is one of the causes of early vision loss among the working population. Retinopathy is a common complication of diabetes. However, preclinical testing of new therapies in animal models of diabetic retinopathy remains complicated by the long time to symptom onset and the high cost of animal housing. One of the solutions is to create models similar to retinopathy without inducing diabetes. One of the approaches for such method is to induce retinopathy using factors such as vascular endothelial growth factor and basic fibroblast growth factor. In our study, we tested the ability of a combination of these factors at low concentrations to induce a stable retinopathy model. The results showed that, despite the fact that the concentration of the factors was reduced, repeated administration of them resulted in a model exhibiting all the key features of the disease.
Keywords
retinopathy, rabbit model, VEGF, bFGF, optical coherence tomography
Article Details
References
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