PHYSIOLOGICAL, BIOCHEMICAL, AND MOLECULAR CHARACTERIZATION OF THE RESPONSE OF POTATO CULTIVAR ASTANALYK TO OSMOTIC STRESS
Main Article Content
Authors
A.K.
Argumbayeva
National Center for Biotechnology, 13/5, Korgalzhyn Hwy., Astana 010000, Kazakhstan
West Kazakhstan Agro-Technical University named after Zhangir Khan, Zhangir khan street 51, Oral, 090009, Kazakhstan
B.R.
Kali
National Center for Biotechnology, 13/5, Korgalzhyn Hwy., Astana 010000, Kazakhstan
L.S.
Abeuova
National Center for Biotechnology, 13/5, Korgalzhyn Hwy., Astana 010000, Kazakhstan
B.N.
Nasiev
West Kazakhstan Agro-Technical University named after Zhangir Khan, Zhangir khan street 51, Oral, 090009, Kazakhstan
Sh.A.
Manabayeva
National Center for Biotechnology, 13/5, Korgalzhyn Hwy., Astana 010000, Kazakhstan
L.N. Gumilyov Eurasian National University, Satpayev st. 2, Astana 010000, Kazakhstan
West Kazakhstan Agro-Technical University named after Zhangir Khan, Zhangir khan street 51, Oral, 090009, Kazakhstan
Abstract
Climate change and the increasing frequency of extreme weather events highlight the need to investigate the mechanisms underlying potato tolerance to water deficit and osmotic stress. This study aimed to evaluate the physiological, biochemical, and molecular responses of potato cultivar Astanalyk to different osmotic agents. Plants were exposed to PEG-6000, sorbitol, and NaCl, after which chlorophyll and malondialdehyde (MDA) contents, catalase (CAT) activity, and the expression levels of the StuNAC, TRE, and OST2 genes were determined in leaves and roots. The stress response varied depending on the type of osmotic agent and tissue. A statistically significant decrease in chlorophyll content was observed only under 8% PEG-6000 treatment. Under this treatment, MDA content reached its maximum value of 12.08 nmol/g, which was 60% higher than the control. The most pronounced increase in CAT activity was observed under 75 mM NaCl treatment, reaching 46.9% above the control level. Treatment with 8% PEG-6000 induced the highest expression of StuNAC, TRE, and OST2, particularly in roots, where their expression levels increased 3.31-, 3.24-, and 2.89-fold, respectively. A strong positive correlation was observed between the expression levels of the investigated genes and CAT activity. These results demonstrate a differential and tissue-specific response of potato cultivar Astanalyk to different osmotic agents and may contribute to the comprehensive assessment of potato stress tolerance.
Keywords
Solanum tuberosum L., cultivar Astanalyk, in vitro, PEG-6000, sorbitol, NaCl, chlorophyll, catalase, malondialdehyde, differential gene expression
Article Details
References
Hanász A., et al. Responses of potato (Solanum tuberosum L.) breeding lines to osmotic stress induced in in vitro shoot culture // Horticulturae. – 2022. – Vol. 8, № 7. – P. 591.
Hu X.-y., et al. A comparative review of the potato industries in China and India // Potato Research. – 2025. – P. 1-24.
Sutula M., et al. Molecular mechanisms underlying defense responses of potato (Solanum tuberosum L.) to environmental stress and CRISPR/Cas-mediated engineering of stress tolerance // Plants. – 2025. – Vol. 14, № 13. – P. 1983.
Sajid Z.A., Aftab F. Improvement of polyethylene glycol, sorbitol, mannitol, and sucrose-induced osmotic stress tolerance through modulation of the polyamines, proteins, and superoxide dismutase activity in potato // International Journal of Agronomy. – 2022. – Vol. 2022, № 1. – P. 5158768.
Azad N., et al. Physiological mechanism of salicylic acid in Mentha pulegium L. under salinity and drought stress // Brazilian Journal of Botany. – 2021. – Vol. 44, № 2. – P. 359-369.
Al-Khayri J., Al-Bahrany A. Growth, water content, and proline accumulation in drought-stressed callus of date palm // Biologia Plantarum. – 2004. – Vol. 48, № 1. – P. 105-108.
Barraza A., Sánchez F. Trehalases: a neglected carbon metabolism regulator? // Plant Signaling & Behavior. – 2013. – Vol. 8, № 7. – P. e24778.
Bianchi G., et al. The unusual sugar composition in leaves of the resurrection plant Myrothamnus flabellifolia // Physiologia Plantarum. – 1993. – Vol. 87, № 2. – P. 223-226.
Fang G., et al. Research progress on physiological, biochemical, and molecular mechanisms of potato in response to drought and high temperature // Horticulturae. – 2024. – Vol. 10, № 8. – P. 827.
Sharma A., et al. Nitric oxide-mediated regulation of oxidative stress in plants under metal stress: a review on molecular and biochemical aspects // Physiologia Plantarum. – 2020. – Vol. 168, № 2. – P. 318-344.
Han Q.Q., et al. Beneficial soil bacterium Bacillus subtilis (GB03) augments salt tolerance of white clover // Frontiers in Plant Science. – 2014. – Vol. 5. – P. 525.
Singh A.K., et al. Genome-wide organization and expression profiling of the NAC transcription factor family in potato (Solanum tuberosum L.) // DNA Research. – 2013. – Vol. 20, № 4. – P. 403-423.
Çelik S. Gene expression analysis of potato drought-responsive genes under drought stress in potato (Solanum tuberosum L.) cultivars // PeerJ. – 2024. – Vol. 12. – P. e17116.
Kanat R., et al. Gene expression analysis for drought tolerance in early stage of potato plant development // Biology. – 2024. – Vol. 13, № 11. – P. 857.
Moon K.B., et al. Transcriptome profiling and characterization of drought-tolerant potato plant (Solanum tuberosum L.) // Molecules and Cells. – 2018. – Vol. 41, № 11. – P. 979-992.
Livak K.J., Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method // Methods. – 2001. – Vol. 25, № 4. – P. 402-408.
Bustin S.A., et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments // Clinical Chemistry. – 2009. – Vol. 55, № 4. – P. 611-622.
Czechowski T., et al. Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis // Plant Physiology. – 2005. – Vol. 139, № 1. – P. 5-17.
Tang X., et al. Selection and validation of reference genes for RT-qPCR analysis in potato under abiotic stress // Plant Methods. – 2017. – Vol. 13, № 1. – P. 85.
Brunner A.M., Yakovlev I.A., Strauss S.H. Validating internal controls for quantitative plant gene expression studies // BMC Plant Biology. – 2004. – Vol. 4. – P. 14.
Jian B., et al. Validation of internal control for gene expression study in soybean by quantitative real-time PCR // BMC Molecular Biology. – 2008. – Vol. 9, № 1. – P. 59.
Nicot N., et al. Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress // Journal of Experimental Botany. – 2005. – Vol. 56, № 421. – P. 2907-2914.
Murashige T., Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures // Physiologia Plantarum. – 1962. – Vol. 15, № 3.
Michel B.E., Kaufmann M.R. The osmotic potential of polyethylene glycol 6000 // Plant Physiology. – 1973. – Vol. 51, № 5. – P. 914-916.
Lichtenthaler H.K., Wellburn A.R. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents // Biochemical Society Transactions. – 1983. – Vol. 11. – P. 591-592.
Shlyk A. Opredeleniye khlorofillov i karotinoidov v ekstraktakh zelenykh list’yev // Biochemical methods in plant physiology. Moscow: Science. − 1971. − Т. 19, № 1. − С. 154 161. [in Russian]
Peng Zhen P.Z., et al. An efficient approach to identify salt tolerance of upland cotton at seedling stage. – 2014.
Dilnur T., et al. Association analysis of salt tolerance in Asiatic cotton (Gossypium arboretum) with SNP markers // International Journal of Molecular Sciences. – 2019. – Vol. 20, № 9. – P. 2168.
Havir E.A., McHale N.A. Biochemical and developmental characterization of multiple forms of catalase in tobacco leaves // Plant Physiology. – 1987. – Vol. 84, № 2. – P. 450-455.
Argumbayeva A., Kali B.R., Abeuova L.S., Nasiyev B.N., Manabayeva S.A. Sravnitel'nyy analiz fiziologicheskikh reaktsiy prorostkov kartofelya na osmoticheskiy stress razlichnoy intensivnosti // Eurasian Journal of Applied Biotechnology. – 2025. – № 3. – С.156-164. [in Russian]
Kalendar R. Comprehensive web-based platform for advanced PCR design, genotyping, synthetic biology, molecular diagnostics, and sequence analysis // Molecular Therapy – Nucleic Acids. – 2025. – Vol. 36, № 4.
Fischer R., Maurer R. Drought resistance in spring wheat cultivars. I. Grain yield responses // Australian Journal of Agricultural Research. – 1978. – Vol. 29, № 5. – P. 897-912.
Ltaief S., Krouma A. Functional dissection of the physiological traits promoting durum wheat (Triticum durum Desf.) tolerance to drought stress // Plants. – 2023. – Vol. 12, № 7. – P. 1420.
Wang G. Agricultural drought in a future climate: results from 15 global climate models participating in the IPCC 4th assessment // Climate Dynamics. – 2005. – Vol. 25, № 7. – P. 739-753.
Im J.S., et al. Salt tolerance and proline accumulation of potato (Solanum tuberosum L.) in vitro plants to NaCl treatment // Journal of Plant Biotechnology. – 2015. – Vol. 42, № 2. – P. 129-134.
Murshed R., et al. Using growth parameters for in-vitro screening of potato varieties tolerant to salt stress. – 2018.
Gopal J., Iwama K. In vitro screening of potato against water-stress mediated through sorbitol and polyethylene glycol // Plant Cell Reports. – 2007. – Vol. 26, № 5. – P. 693-700.
Mawia R., et al. Impact of sorbitol-induced osmotic stress on some biochemical traits of potato in vitro // Iraqi Journal of Agricultural Sciences. – 2020. – Vol. 51, № 4. – P. 1038.
Wellpott K., et al. Liquid in vitro culture system allows gradual intensification of osmotic stress in Solanum tuberosum through sorbitol // Plant Cell, Tissue and Organ Culture. – 2024. – Vol. 157, № 1. – P. 12.