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D.A. Gritsenko

Institute of plant biology and biotechnology, Timiryazev str., 45, Almaty, 050040, Kazakhstan

N.D. Deryabina

Institute of plant biology and biotechnology, Timiryazev str., 45, Almaty, 050040, Kazakhstan

N.N. Galiakparov

Institute of plant biology and biotechnology, Timiryazev str., 45, Almaty, 050040, Kazakhstan


Grapevine virus A belongs to the Vitivirus genus. Grapevine virus A is involved in the etiology of Kober stem grooving, a disease of the grapevine rugose wood complex.  The grapevine virus A genome (7.4 kb) consists of five open reading frames. The ORF2 function is not currently investigated. We attempted to develop a vector, based on grapevine virus A by cloning the gene encoding the capsid protein of apple chlorotic leaf spot virus in ORF2. Modification of grapevine virus A genome has not affected amplification or movement of virus through the plant. Symptoms of infection were detected 6-7 day after agroinfiltration. Nevertheless, immunoblotting has not detected the capsid protein of the capsid protein of apple chlorotic leaf spot virus either in the infiltrated leaves or in the upper leaves on the 15th day after agroinfiltration. It may be explained by the weak subgenomic ORF2 promoter. In addition, the genetic stability of modified grapevine virus A after K gene insertion was confirmed.


Grapevine virus A, ORF2, capsid protein, movement protein, apple chlorotic leaf spot virus

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Martelli G.P., Minafra A. and Sardarelli P. Vitivirus, a new genus of plant viruses. Archives of Virology,1997, vol. 142, рр. 1929-1932. 9672652.

Chevalier S., Greif C., Bass E. and Walter B. Investigations on the aetiology of Kober stem grooving.Extended Abstracts, XIth ICVG Meeting (Nyon, Switzerland), рр.49-49.

Conti M., Milne R.G., Luisoni E. and Boccardo G. A closterovirus from a stem-pitting diseased grapevine. Phytopathology, 1980, vol. 79, рр.394-399. doi:10.1094/Phyto-70-394.

Garau R., Prota U., Piredda R. and Boscia D. On the relationship between Kober stem groovi ng and grapevine virus A. Vitis, 1994, vol. 33, рр. 161-163.

Engelbrecht D.J., Kasdorf G. Occurrence and transmission of grapevine virus A in South African grapevines.S Afr J Enol Vitic, 1987,vol. 8, рр.23-29.doi:10.21548/8-1-2325.

Garau R., Prota V.A., Boscia D., Fiori M., ProtaU. А new vector of trichoviruses A and B.Vitis, 1995,vol. 34, рр. 67-68.

Rosciglione B., Castellano M.A., Martelli G.P., Savino V., Cannizzaro G. Mealybug transmission of grapevine virus A.Vitis, 1983,vol. 22, рр.331-347 .doi:10.1094/PHYTO-100-8-0830.

Tanne E., Ben-Dov Y., Raccah B. Transmission of the corky-bark disease by the mealybug Planococcusficus.Phytoparasitica, 1989, vol. 17, рр.55.doi:10.1094/ PHYTO-98-10-1093.

Galiakparov N., Tanne E., Sela I., Gafny R. Infectious RNA transcripts from Grapevine virus A cDNA clone.Virus Genes, 1999,vol. 19, рр.235-242.doi:10.1023/A:1008192831883.

Galiakparov N., Goszczynski D.E., Che X., Batuman O., Bar-Joseph M.,Mawassi M. Two classes of subgenomic RNA of grapevine virus A produced by internal controller elements.Virology, 2003, vol. 312,рр.434-448.doi:10.1016/S0042-6822(03)00239-3.

Chiba M., Reed J.C., Prokhnevsky A.I., Chapman E.J., Mawassi M., Koonin E.V., Carrington J.C., Dolja V.V. Diverse suppressors of RNA silencing enhance agroinfection by a viral replicon. Virology, 2006, vol. 346, рр.7-14. doi:10.1016/j.virol.2005.09.068.

Zhou Z.Sh., Dell’Orco M., Saldarelli P., Turturo C., Minafra A., Martelli G.P. Identification of an RNA-silencing suppressor in the genome of Grapevine virus A. Journal of General Virology, 2006,vol. 87, рр. 2387-2395. doi:10.1099/vir.0.81893-0.

Haviv S., Iddan Y., Goszczynski D.E., Mawassi M. The ORF5 of Grapevine virus A is involved in symptoms expression in Nicotiana benthamiana plants. Annals of applied biology,2012, vol. 160, рр.181-190. doi:10.1111/j.1744-7348.2012.00531.x.

Galiakparov N., Tanne E., Sela I., Gafny R. Functional analysis of the grapevine virus A genome. Virology, 2003, vol. 306, рр.42-50. doi:10.1016/S0042-6822(02)00019-3.

Gleba Y.Y., Tusé D., Giritch A. Plant viral vectors for delivery by Agrobacterium.Curr Top Microbiol Immunol, 2014,vol. 375, рр. 155-192.doi:10.1007/82_2013_352.

Haviv S., Galiakparov N., Goszczynski D.E., Batuman O., Czosnek H., Mawassi M. Engineering the genome of Grapevine virus A into a vector for expression of proteins in herbaceous plants. J Virol Methods, 2006, vol. 132, рр. 227-231.doi:10.1016/j.jviromet.2005.10.020.

Lacks S., Greenberg B. Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation. J. Mol. Biol.114, 1997, рр. 153-168. 20509.

Gritsenko D.A., Kenzhebekova R.T., Deryabina N.D.,Galiakparov N.N. CozdanieT-vektora dlya pryamogo klonirovaniya PTsR-produktov. Eurasian journal of applied biotechnology, 2016, vol. 4, pp.43-50. doi:10.11134/btp.4.2016.6.

Kopytina D.A., Galiakparov N.N. Izuchenie sposobnosti virusa A vinograda infitsirovat Nicotiana benthamiana pri agrobakterialnoy infiltratsii.Vestnik KazNU, 2014, vol. 1, pp. 195-199.

Wise A.A.1, Liu Z., Binns A.N. Three methods for the introduction of foreign DNA into Agrobacterium. Methods Mol Biol., 2006, vol. 343, рр. 43-53.doi:10.1385/1-59745-130-4:43.10.1385/1-59745-130-4:43.

Teimourpour R., Sadeghian A., Meshkat Z., Esmaelizad M., Sankian M., Jabbari A.R. Construction of a DNA Vaccine Encoding Mtb32C and HBHA Genes of Mycobacterium tuberculosis. Jundishapur J Microbiol., 2015, vol. 8, рр. 1-7.doi:10.5812/jjm.21556.

Rubinson E., Galiakparov N., Radian S., Sela I., Tanne E., Gafny R. Serological detection of grapevine virus a using antiserum to a nonstructural protein, the putative movement protein. Phytopathology, 1997, vol. 87, рр.1041-1045.doi:10.1094/PHYTO.1997.87.10.1041.