COMPARATIVE STUDY OF ELISA STOP SOLUTIONS BASED ON MINERAL ACIDS
Main Article Content
Authors
B.M. Ismagambetov
OtarBioPharm LLP, 5 Gvardeysky Settlement, Korday District, Zhambyl Region, 080409, Republic of Kazakhstan
S.E. Shomanova
OtarBioPharm LLP, 5 Gvardeysky Settlement, Korday District, Zhambyl Region, 080409, Republic of Kazakhstan
I.A. Akhazhanova
OtarBioPharm LLP, 5 Gvardeysky Settlement, Korday District, Zhambyl Region, 080409, Republic of Kazakhstan
N.B. Esimbekova
OtarBioPharm LLP, 5 Gvardeysky Settlement, Korday District, Zhambyl Region, 080409, Republic of Kazakhstan
Z.D. Ershebulov
OtarBioPharm LLP, 5 Gvardeysky Settlement, Korday District, Zhambyl Region, 080409, Republic of Kazakhstan
Abstract
A comparative study of ELISA stop solutions prepared on the basis of hydrochloric, orthophosphoric and sulfuric acids was performed using a competitive ELISA model for detection of antibodies against bluetongue virus. It was demonstrated that the chemical nature and concentration of the acid significantly affect the optical density of negative (K-) and positive (K+) controls, the time-dependent change of the signal, and the ratio between negative and positive controls.
Hydrochloric acid solutions within the range of 0.5-4.0 M provided a high initial signal for K- (approximately 2.0 OD units), limited optical density variation over 30 minutes (3-8%), and the highest control ratio values (50-90). Orthophosphoric acid resulted in an increase of the positive control signal over time, whereas sulfuric acid caused a decrease in both K- and K+ signals, leading to narrowing of the dynamic range.
The findings confirm that the selection of the stop solution is a critical factor in ELISA standardization and directly influences the analytical reliability of the diagnostic test system.
Keywords
enzyme-linked immunosorbent assay, stop solution, mineral acids, hydrochloric acid, orthophosphoric acid, sulfuric acid, competitive ELISA, bluetongue virus
Article Details
References
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