Priming of Solanum Tuberosum in vitro plants with PVY-specific interfering RNAs activates anti-viral resistance
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Authors
M. Sutula
National Scientific Shared Laboratory of NJSC Sarsen Amanzholov East Kazakhstan University, Ust-Kamenogorsk, Kazakhstan, 070004, 55 Kazakhstan
T. Khosnutdinova
National Scientific Shared Laboratory of NJSC Sarsen Amanzholov East Kazakhstan University, Ust-Kamenogorsk, Kazakhstan, 070004, 55 Kazakhstan
Yе. Zhakmanova
National Scientific Shared Laboratory of NJSC Sarsen Amanzholov East Kazakhstan University, Ust-Kamenogorsk, Kazakhstan, 070004, 55 Kazakhstan
G. Dolanbayeva
National Scientific Shared Laboratory of NJSC Sarsen Amanzholov East Kazakhstan University, Ust-Kamenogorsk, Kazakhstan, 070004, 55 Kazakhstan
X. Bogdanova
National Scientific Shared Laboratory of NJSC Sarsen Amanzholov East Kazakhstan University, Ust-Kamenogorsk, Kazakhstan, 070004, 55 Kazakhstan
Ye. Yessilbekova
National Scientific Shared Laboratory of NJSC Sarsen Amanzholov East Kazakhstan University, Ust-Kamenogorsk, Kazakhstan, 070004, 55 Kazakhstan
G. Komekova
Department of Biology of the Graduate School of IT and Technologies of NJSC Sarsen Amanzholov East Kazakhstan University, Ust-Kamenogorsk, Kazakhstan, 070004, 55 Kazakhstan
Zh. Kabatayeva
Department of Biology of the Graduate School of IT and Technologies of NJSC Sarsen Amanzholov East Kazakhstan University, Ust-Kamenogorsk, Kazakhstan, 070004, 55 Kazakhstan
Abstract
Helper component-proteinase (HC-Pro) is a multifunctional suppressor protein synthesized by potato virus Y (PVY), which is able to neutralize the protective mechanisms of RNA interference (RNA-i) in potato plants, thereby causing systemic infection of the host plant and significant damage to the tuber material. This article demonstrates how one of the main functions of HC-Pro, the capture and retention of short interfering RNAs, 21-23 nt in size (siRNA) on the surface of protein subunits, can be used to create virus-resistant potato plants in vitro. The proposed method is based on the selective dissociation of the HC-Pro/siRNA complex from PVY-infected plants and siRNA-priming of healthy plants. Purified preparations of PVY-specific siRNAs were obtained using column gel filtration followed by immunoprecipitation and phenol-chloroform extraction. Injections of siRNA into the leaf plate of virus-free potato microplants, cultivars bred at Amanzholov University, led to a decrease in the accumulation of viral particles in the cytoplasm of plants inoculated with the wild PVY strain and the formation of resistance to PVY for the entire growing season.
Keywords
Solanum tuberosum, HC-Pro, PVY, RISC, siRNA, RNA-interference
Article Details
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