COMPARATIVE ANALYSIS OF GENETIC RELATEDNESS OF VIBRIO CHOLERAE STRAINS ISOLATED FROM THE WEST KAZAKHSTAN, MANGYSTAU AND TURKESTAN REGIONS OF KAZAKHSTAN
Main Article Content
Authors
M.M. Kulbayeva
LLP "National Scientific Center for Especially Dangerous Infections", Almaty, Republic of Kazakhstan
R.S. Mussagaliyeva
LLP "National Scientific Center for Especially Dangerous Infections", Almaty, Republic of Kazakhstan
Z.B. Zhumadilova
LLP "National Scientific Center for Especially Dangerous Infections", Almaty, Republic of Kazakhstan
G.Zh. Tokmurziyeva
LLP "National Scientific Center for Especially Dangerous Infections", Almaty, Republic of Kazakhstan
A.K. Izekenova
Al-Farabi Kazakh National University, 94 Tole Bi street, Almaty, 050006, Republic of Kazakhstan
B.K. Toyzhanov
LLP "National Scientific Center for Especially Dangerous Infections", Almaty, Republic of Kazakhstan
E.Zh. Begimbayeva
LLP "National Scientific Center for Especially Dangerous Infections", Almaty, Republic of Kazakhstan
I.B. Utepova
LLP "National Scientific Center for Especially Dangerous Infections", Almaty, Republic of Kazakhstan
Z.Zh. Abdel
LLP "National Scientific Center for Especially Dangerous Infections", Almaty, Republic of Kazakhstan
D.M. Otebay
¹LLP "National Scientific Center for Especially Dangerous Infections", Almaty, Republic of Kazakhstan
Abstract
Cholera is an acute anthroponotic infection caused by Vibrio cholerae, characterized by severe diarrhea and dehydration. Despite advances in public health and sanitation, cholera remains a major global health concern, particularly in Asia and Africa. In Kazakhstan, the pathogen is periodically detected in surface and wastewater, emphasizing the need for continuous molecular surveillance. The study included 33 isolates of Vibrio cholerae obtained from the West Kazakhstan, Mangystau, and Turkestan regions of the Republic of Kazakhstan. Phenotypic and biochemical identification was performed using the VITEK 2 Compact 30 automated system (bioMérieux, USA), achieving 97,0 % accuracy. The aim of this research was to perform a comparative phylogenetic analysis based on sequencing of the 16S rRNA gene fragment and construction of phylogenetic trees using the Neighbor-Joining method in MEGA 7.0. The results demonstrated a high level of nucleotide identity among the isolates (99.3-100,0 %), indicating strong genetic conservatism and prolonged circulation of clonally related El Tor lineages in Kazakhstan. Phylogenetic reconstruction revealed two stable clusters (A and B) with limited intraspecific variability and no clear geographical differentiation. The study concludes that 16S rRNA sequencing is useful for primary identification of V. cholerae strains, but higher-resolution approaches such as MLST, MLVA, or WGS are required for fine-scale epidemiological and evolutionary characterization.
Keywords
Vibrio cholerae, strain, VITEK, genotype, phylogeny, analysis
Article Details
References
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