OPTIMIZATION OF THE CULTURE METHOD FOR THE MOST EFFECTIVE SYNTHESIS OF POLYHYDROXYBUTYRATE BY THE BACILLUS ARYABHATTAI RAF 5 STRAIN AND ANALYSIS OF BARRIER PROPERTIES OF THE OBTAINED MEMBRANE
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Authors
A. Rysbek
National Center for Biotechnology, 13/5 Korgalzhynskoye Road, Astana, 010000
A. Shamukhan
National Center for Biotechnology, 13/5 Korgalzhynskoye Road, Astana, 010000
S. Abeldenov
National Center for Biotechnology, 13/5 Korgalzhynskoye Road, Astana, 010000
Abstract
- The method of periodic updating (PU) or the periodic addition (PA) of fresh medium is considered as a promising technique for long-term and effective production of polyhydroxybutyrate (PHB) synthesized by different soil bacteria. In our previous studies, we reported PHB-producing potential of the Bacillus aryabhattai strain RAF 5 IMD B - 462 isolated from the chestnut soils in Astana city, Kazakhstan. The current study reports on the optimization of the RAF 5 cultivation using PU technology to obtain a high yield of PHB and biomass, as well as on the study of a membrane obtained on the basis of PHB. The accumulation of dense PHB granules in cell cytosol was confirmed by Transmission Electron Microscopy (TEM) analyses; more than 10 granules per cell were found in the cytosol of RAF 5. PHB was further extracted using the solvent extraction method. The highest PHB yield and biomass obtained after 72-hour incubation were 18.03 g/L and 20.04 g/L, respectively, when PU was applied. The highest PHB titer after 120-hour incubation was 3.76 g/L, when RAF 5 was growing in a conventional culture medium NB. The obtained PHB was further utilized to make a membrane sample which was subjected to water vapor, oxygen and carbon dioxide gas permeability analyses showing the promising results. In conclusion, this data proposes potential application might be utilized as a promising material to produce membranes.
Keywords
Bacillus aryabhattai, Polyhydroxybutyrate, Permeability of membranes, Periodic updating (PU), Transmission Electron Microscopy (TEM), Soil bacteria of Kazakhstan
Article Details
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