OBTAINING SPECIFIC ANIMAL BLOOD SERUM TO THE CAUSATIVE AGENT OF EPIZOOTIC LYMPHANGOITIS IN EQUINES
Main Article Content
Authors
E.O. Abduraimov
JSC National Holding QazBioPharm Astana, Kazakhstan
M.Zh. Kaukarbaeva
Research Institute for Biological Safety Problems, Gvardeisky, Kazakhstan
A.S. Rsaliev
JSC National Holding QazBioPharm Astana, Kazakhstan
B.K. Umuraliev
Research Institute for Biological Safety Problems, Gvardeisky, Kazakhstan
A.A. Isakhan
Research Institute for Biological Safety Problems, Gvardeisky, Kazakhstan
N.K. Orazimbetova
Research Institute for Biological Safety Problems, Gvardeisky, Kazakhstan
Kh.B. Abeuov
Research Institute for Biological Safety Problems, Gvardeisky, Kazakhstan
Zh.B. Kondibaeva
Research Institute for Biological Safety Problems, Gvardeisky, Kazakhstan
Zk.K. Koshemetov
Research Institute for Biological Safety Problems, Gvardeisky, Kazakhstan
A.S. Zhakypbek
Research Institute for Biological Safety Problems, Gvardeisky, Kazakhstan
A.K. Nakhanov
Research Institute for Biological Safety Problems, Gvardeisky, Kazakhstan
Abstract
Selection of animals for obtaining specific blood serums is an important step. Before the start of immunization, blood samples were taken from potential donors for testing using enzyme-linked immunosorbent assay (ELISA), diffusion precipitation reaction (DPR) and prolonged complement fixation reaction (PCFR) to detect antibodies to other viral and bacterial infections. It has been experimentally established that antibodies to the pathogens of equine epizootic lymphangitis (ELL), peste des petits ruminants, sheep pox and anthrax were not detected in the blood serum of rabbits, goats and sheep used to obtain specific blood serum. This is necessary to exclude possible cross-reactions when using the obtained immune sera in serological reactions. To obtain specific sera in the experiment, purified antigens of the cultures of strains “T”, “3724 K” and “3730 K” of the causative agent of ELL were used, which have activity in the RDP within the range of 1:16-1:32 and in the RSC of at least 1:40-1:80.
To obtain a specific serum, 11 immunization schemes were tested, which differed in the methods of immunization, the concentration and volume of the administered antigen, the intervals between administrations, and the different strains of diseases used to administer them.
Experimentally, we managed to obtain specific and active serums from rabbits, goats and sheep. The activity of immune serums in rabbit #6 DPR was 1:32, in PCFR – 1:128, goat #1 in DPR – 1:16, in PCFR 1:64 and sheep #1 in DPR 1:8, in PCFR 1:32. These diagnostic serums will be used to establish laboratory methods for detecting the antigen of the ELL pathogen in the biological materials being studied.
Keywords
equine epizootic lymphangitis, specific serum, hyperimmunization, strain, purified antigen
Article Details
References
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