ANTI-CANCEROUS POTENTIAL OF POLYSACCHARIDES DERIVED FROM WHEAT CELL CULTURE
Main Article Content
Authors
A.S. Murtazina
Department of General Immunology, Faculty of Medicine, Asfendyarov Kazakh National Medical University, 050012, Almaty, Kazakhstan.Research
Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research, University of Granada, Granada E-18100, Spain;
J.A. Marchal
Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research, University of Granada, Granada E-18100, Spain;
A.S. Tarabayeva
Department of General Immunology, Faculty of Medicine, Asfendyarov Kazakh National Medical University, 050012, Almaty, Kazakhstan.Research
E.Zh. Bitanova
Department of General Immunology, Faculty of Medicine, Asfendyarov Kazakh National Medical University, 050012, Almaty, Kazakhstan.Research
G. McDougall
Plant Biochemistry and Food Quality Group, Environmental and Biochemical Sciences Department The James Hutton Institute, Invergowrie, Dundee
H.B. Tassi
Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research, University of Granada, Granada E-18100, Spain;
N.K. Bishimbayeva
Al-Farabi Kazakh National University, Institute of Biology and Biotechnology, 050040, Almaty, Kazakhstan;
Abstract
There is a global need to discover effective anti-cancerous compounds from natural sources. Cultivated wheat cells can be a valuable source of non-toxic or low toxic plant-derived polysaccharides. In this study we evaluated the antitumor ability of seven fractions of wheat cell culture polysaccharides (WCCPSs) in the HCT-116 colon cancer cell line.
Almost all (6/7) fractions had an inhibitory effect on the proliferation of colon cancer cells, and two fractions (A-b and A-f) had considerable therapeutic indexes. The WCCPS fractions induced cell cycle arrest in the G1 phase and induced different rates of apoptosis (<48%). Transmission and scanning electron microscopy revealed that WCCPS fractions caused apoptotic changes in the nucleus and cytoplasm, including damage to mitochondria and external morphological signs of apoptosis.
In addition, the WCCPSs induced an increase in the levels of Bax, cytochrome c, caspases 8 and 3, indicating that cell death was assessed through intrinsic and extrinsic pathways of apoptosis. Furthermore, some fractions caused a significant decrease of c-Myc, b-catenin, NFkB2 and HCAM (CD 44) levels, indicating enhanced cell differentiation. Thus, for the first time, our results provide a proof of concept of the anti-cancer capacity of WCCPS fractions in colorectal cancer.
This research was supported by the FEDER Operational Program 2020/Junta de Andalucía-Consejería de Economía y Conocimiento/ Project (B-CTS-562-UGR20) and the Chair “Doctors Galera-Requena in cancer stem cell research” (CMC-CTS963); Erasmus+ Mobility Program; “Bolashak” Presidential Scholarship of the Republic of Kazakhstan for scientific work abroad; Scottish Government’s Rural and Environment Science and Analytical Services (RESAS) division.