INFLUENCE OF PHYSICOCHEMICAL FACTORS ON THE SARS-COV-2 STRAIN ISOLATED FROM A DOG
Main Article Content
Authors
S.O. Sadikaliyeva
LLP "Research Institute for Biological Safety Problems" Gvardeysky 080409, Kazakhstan
National Academy of Sciences of the Kyrgyz Republic, Kyrgyzstan
E.Zh. Kalimolda
LLP "Research Institute for Biological Safety Problems" Gvardeysky 080409, Kazakhstan
Zh.S. Abay
LLP "Research Institute for Biological Safety Problems" Gvardeysky 080409, Kazakhstan
Ye.A. Shayakhmetov
LLP "Research Institute for Biological Safety Problems" Gvardeysky 080409, Kazakhstan
S.U. Moldagulova
LLP "Research Institute for Biological Safety Problems" Gvardeysky 080409, Kazakhstan
K.A. Shorayeva
LLP "Research Institute for Biological Safety Problems" Gvardeysky 080409, Kazakhstan
K.K. Jekebekov
LLP "Research Institute for Biological Safety Problems" Gvardeysky 080409, Kazakhstan
A.D. Omurtay
LLP "Research Institute for Biological Safety Problems" Gvardeysky 080409, Kazakhstan
A.T. Zhunushov
National Academy of Sciences of the Kyrgyz Republic, Kyrgyzstan
N.S. Kozhabergenov
LLP "Research Institute for Biological Safety Problems" Gvardeysky 080409, Kazakhstan
B.A. Yespembetov
LLP "Research Institute for Biological Safety Problems" Gvardeysky 080409, Kazakhstan
M.M. Kassenov
LLP "Research Institute for Biological Safety Problems" Gvardeysky 080409, Kazakhstan
A.S. Nurpeisova
LLP "Research Institute for Biological Safety Problems" Gvardeysky 080409, Kazakhstan
Abstract
This article presented the results of a study on the impact of physicochemical factors on the biological activity of the SARS-CoV-2 coronavirus isolated from a dog in the Republic of Kazakhstan. The results indicated that storing the virus at a temperature of -70°C led to a decrease in viral activity, similar to the effect observed when stored at room temperature. Storage at +40°C resulted in complete inactivation of the virus. The study on the effect of various concentrations of ionic and non-ionic detergents revealed that exposure to the ionic detergent DOC at concentrations of 0.1% and 0.5% caused a decrease in viral activity, while no change was observed at a concentration of 0.01%. The detergent SDS, at concentrations of 0.01%, 0.1%, and 0.5%, reduced the biological activity of the virus from the initial titer (5.25 lg TCID50/cm³) to 4.00 lg TCID50/cm³. Similarly, the use of non-ionic detergents, Triton X-100 and Tween-20, led to a reduction in viral activity from the initial titer to 4.50 lg TCID50/cm³.
The effect of pH in acidic, neutral, and alkaline environments on the biological activity of the virus was also studied. A decrease in viral activity was observed at acidic and alkaline pH levels, whereas a neutral pH environment had no impact on viral activity.
Keywords
coronavirus infection, physicochemical properties, biological activity.physico-chemical factors, isolation from the virus, virus resistance, coronavirus infection, virus resistance, biological activity
Article Details
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