INDICATION, IDENTIFICATION AND INACTIVATION SALMONELLA ABORTUS-OVIS
Main Article Content
Authors
А.К. Mussayeva
Kazakh Scientific-Research Veterinary Institute, Rayymbek Ave. 223, Almaty, 050016, Kazakhstan
N.N. Egorova
Kazakh Scientific-Research Veterinary Institute, Rayymbek Ave. 223, Almaty, 050016, Kazakhstan
M.O. Yerishov
Kazakh Scientific-Research Veterinary Institute, Rayymbek Ave. 223, Almaty, 050016, Kazakhstan
A.A. Tlepov
Kazakh Scientific-Research Veterinary Institute, Rayymbek Ave. 223, Almaty, 050016, Kazakhstan
M.M. Kassenov
Kazakh Scientific-Research Veterinary Institute, Rayymbek Ave. 223, Almaty, 050016, Kazakhstan
Abstract
The article presents the results of identification of an epizootic culture of Salmonella abortus-ovis (A) 58 isolated from parenchymatous organs of an aborted ovine fetus, inv. No. KZH 210397333, delivered from the village of Senokosnoye, Osakarovsky district, Karaganda region. To isolate a pure culture of salmonella from pathological material from the abort fetus of a ovine, the method of stepwise selection of colonies according to Drigalsky was used. The identification of the isolated culture was carried out on the basis of studying the cultural-morphological, biochemical, and antigenic properties of Salmonella abortus-ovis culture (A) 58. The culture was agglutinated with polyvalent and monoreceptor salmonella sera. According to the results of typing in AR, the culture isolated from the aborted ovine fetus was assigned to the genus Salmonella, the species abortus-ovis, serological group B. The article presents the results of experiments to optimization the process of salmonella cultivation. The passive matrix cultures of salmonella of the second generation in the exponential growth phase were used to cultivate the epizootic S. abortus-ovis (A) 58 strain. Periodic cultivation of salmonella ensured the physiologically active state of the culture, an increase in the maximum growth rate, a reduction in lag phase and a minimum generation time. The salmonella inactivation regime has been worked out, followed by seeding into liquid and dense nutrient media to determine the completeness of inactivation. It was found that effective inactivants are 10% solution of dimerethylenimine (DEI) and a 10% solution of formalin. The article presents the conditions of reactivation and cultivation of the S. abortus—ovis strain (A) 58.
Keywords
infection, salmonella, abortions, indication, identification, inactivation
Article Details
References
SHimanov V.G. Ginekologicheskie zabolevaniya kak odna iz prichin yalovosti ovec. ZH. Karakulevodstvo i zverovodstvo.—1954.-№2.- S.50-52.
SHahov A.G. Ekologicheskie problemy patologii sel'skohozyajstvennyh zhivotnyh. – Voronezh.-1997.-. S.17-20.
Terent'ev F.A., Markov A.A. i dr. Infekcionnye i invazionnye bolezni ovec i koz. – M.-1951.- S.218-219.
Konopatkin A.A., Bakulov I.A., Nujkin YA.V i dr, Epizootologiya i infekcionnye bolezni sel'skohozyajstvennyh zhivotnyh. – M.: Kolos, 1984. –S. 436-437.
Dzhambulatov Z.M. Sal'monellez ovec v yuzhnyh regionah Rossii: Avtoreferat diss. dokt. Vet. nauk. Mahachkala, 2004.- S.93-105.
Mikailov M.M., Musiev D.G., Dzhambulatov Z.M. Metodicheskie rekomendacii po diagnostike, lecheniyu i profilaktike sal'monelleza melkogo rogatogo skota. – Mahachkala.-2011 S.-19.
Alikaev V.A., Dul'nev V.I. i dr. Profilaktika i lechenie boleznej molodnyaka sel'skohozyajstvennyh zhivotnyh. M.-1968.- S. 325-330.
Latyshev S.N. Osobennosti epizooticheskogo processa pri sal'monelleze i kolibakterioze yagnyat: diagnostika, profilaktika i terapiya: Avtoreferat diss. kand. Vet. nauk. Stavropol'.-2009.- S. - 22.
Kadymov R.A. i dr. Infekcionnye bolezni ovec. M.: Agropromizdat, 1987. - S.151-153.
Gnezdilova L.A. Epizootologicheskaya harakteristika, diagnostika i profilaktika smeshannyh infekcij ovec s sindromom porazheniya reproduktivnyh organov. Avtoreferat diss. dokt. Vet. nauk.- Moskva.-2005. – S. 21-30.
Bessarabov, E.S., Sidorchuk A.A. i dr. Infekcionnye bolezni zhivotnyh. -- M.: Kolos.- 2007. -671 S.
Kanteri V.M. Teoreticheskie osnovy tekhnologii mikrobiologicheskih proizvodstv. - M.: Agropromizdat.-1991.- S. 272.
Matvienko B.A., Kenzhibaev M.A. Voprosy immunologii pri associirovannoj vakcinacii protiv paratifa ovec, brucelleza i listerioza.—Vestnik s/h nauki Kazahstana.- 1967.- №7.- S. 41-43.
Samujlenko A.YA. , Ruban E.A. Osnovy biotekhnologii proizvodstva biologicheskih preparatov (Teoreticheskie osnovy, oborudovanie, tekhnologicheskie linii). M.- 2000.- 782 s.
YArcev M.YA. Razrabotka tekhnologii vakcin protiv pasterelleza zhivotnyh i ptic. ZH.Veterinariya-1996- N.2.- s. 17-19.
YArcev M.YA., Raevskij A.A.,, Anisimova I.V. Patent RF № 2129016 (1999)
Rozhdestvenskaya T.N., Karimova L., Pankratov S.V., Ruzina A.V., Tomina E.V. Sovremennye podhody k izgotovleniyu inaktivirovannyh vakcin.- ZH. Agrarnaya nauka.-2022;1(7-8): 68-73.
Ruban E.A. i dr. pod red. A.YA. Samujlenko. Promyshlennaya tekhnologiya proizvodstva protivobakterijnyh preparatov. – M.: IKC «Akademkniga», 2006.-267 s.
Susskij E.V. i dr. Sistema posevnyh materialov-faktor stabil'nosti serijnogo proizvodstva biopreparatov/ZH. Veterinarnaya patologiya, 2013. - №3.-S.43-49.
SHkol'nikov E.E., Anisimova L.V., Pavlenko I.V. i dr. Vakcina polivalentnaya protiv sal'monelleza produktivnyh zhivotnyh. Patent RF RU2764118S1, 2021 g- 3 s.
Opredelitel' Bergey's Manual of Systematic Bacteriology /Department of Microbiology and Molecular Genetics: Michigan State University: USA, 2005, Volum 2. Part V. p. 764 – 799.
Antonov B.I. Metodicheskie ukazaniya po bakteriologicheskoj diagnostike sal'monellezov zhivotnyh. V kn. Laboratornye issledovaniya v veterinarii.- M.: Agropromizdat.-1986.-S.177-192.
Prikaz Ministra sel'skogo hozyajstva Respubliki Kazahstan ot 30 aprelya 2015 goda № 7-1/393 ob utverzhdenii Pravil otbora prob, peremeshchaemyh (perevozimyh) ob"ektov i biologicheskogo materiala, utverzhdennye.