APPLICATION OF THE DNA BARCODING METHOD FOR IDENTIFICATION AND TAXONOMIC ANALYSIS OF THE LAMIACEAE FAMILY
Main Article Content
Authors
N.B. Zhumabay
LLP «National center for biotechnology», Qorgalzhyn highway 13/5, Astana, 010000, Kazakhstan
N.N. Gubaidullin
LLP «National center for biotechnology», Qorgalzhyn highway 13/5, Astana, 010000, Kazakhstan
M. Yu. Ishmuratova
Non-Commercial JSC “E.A. Buketov Karaganda University”, Kazakhstan, Karaganda, University street, 28
S.A. Manabayeva
LLP «National center for biotechnology», Qorgalzhyn highway 13/5, Astana, 010000, Kazakhstan
Abstract
The Lamiaceae family is one of the largest and most important among the aromatic plants, including many species with pronounced pharmacological properties. Plants of this family, such as mint, rosemary and thyme, are widely used in traditional and modern medicine due to their therapeutic and biochemical properties. The importance of correct species identification is emphasized by the need to preserve the biodiversity and sustainable use of the resources of this family. In this work, plants of Lamiaceae family growing in the territory of the Karaganda region were studied by DNA barcoding. The studies were conducted on the basis of nucleotide sequences of rbcL and trnH-psbA chloroplast DNA barcodes using the methods of paired genetic distances, BLASTn and phylogenetic tree based on the analysis of maximum parsimony. The high efficiency of the rbcL locus was demonstrated for the identification of higher taxonomic groups, such as subfamilies and tribes, while the intergenic spacer trnH-psbAhasproved to be effectiveat the level of speciesandgenus level.
Keywords
Labiatae - Lamiaceae family, DNA barcoding, rbcL, trnH-psbA, phylogenetic analysis
Article Details
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