SELECTION OF STRONG POSITIVE SERUM SAMPLES FROM CATTLE INFECTED WITH BOVINE VIRAL DIARRHEA
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Authors
E.E. Bashenova
«Kazakh Scientific Research Veterinary Institute» LLP, Almaty, 050016, Republic of Kazakhstan
R.K. Nissanova
«Kazakh Scientific Research Veterinary Institute» LLP, Almaty, 050016, Republic of Kazakhstan
A.Zh. Zharmukhametova
«Kazakh Scientific Research Veterinary Institute» LLP, Almaty, 050016, Republic of Kazakhstan
P.B. Akshalova
«Kazakh Scientific Research Veterinary Institute» LLP, Almaty, 050016, Republic of Kazakhstan
M.S. Serikov
«Kazakh Scientific Research Veterinary Institute» LLP, Almaty, 050016, Republic of Kazakhstan
S.B. Mamanova
«Kazakh Scientific Research Veterinary Institute» LLP, Almaty, 050016, Republic of Kazakhstan
V.V. Kirpichenko
«Kazakh Scientific Research Veterinary Institute» LLP, Almaty, 050016, Republic of Kazakhstan
Abstract
Viral diarrhoea is one of the most common and economically important diseases in cattle, causing significant losses in productivity and increased mortality in young livestock. Effective diagnosis of the disease requires the use of standardised sera that ensure high accuracy and reproducibility of laboratory results. Selection of sera to obtain standard sera for the diagnosis of bovine viral diarrhea represents a key challenge in the development of a standard sera panel. During monitoring in some regions of the Republic of Kazakhstan (Almaty, Mangistau, Karaganda, Turkistan oblasts) 399 samples of biomaterial from cattle were collected in private subsidiary farms and serological method of research was applied. In a serological test, specific diarrhea virus antibodies were detected in 310 serum samples. Comparative studies of sensitivity and stability of the positive sera with positive standard serum from the kit (ID Vet) were performed. During selection of sera, several factors were determined: specificity and sensitivity of the sera used, with consideration of the peculiarities of the epizootic situation in the region. This research work culminates in the production of a positive serum for the candidate from field-derived natural BVDV disease infections, and shortens the production cycle and saves costs.
Keywords
вирус, диарея, крупный рогатый скот, серологические исследование, стандартные сыворотки, панель сывороток
Article Details
References
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