MOLECULAR-GENETIC IDENTIFICATION OF CAUSAL AGENTS OF FUNGAL DISEASES ON POTATO AND TOMATO COLLECTED UNDER PRODUCTION STORAGE
Main Article Content
Authors
Zh.T. Zhanybekova
National Center for Biotechnology, Korgalzhyn road, 13/5,Nur-Sultan , 010000, Kazakhstan
M. Imanbekova
National Center for Biotechnology, Korgalzhyn road, 13/5,Nur-Sultan , 010000, Kazakhstan
A.M. Aitkulova
National Center for Biotechnology, Korgalzhyn road, 13/5,Nur-Sultan , 010000, Kazakhstan
E.V. Zholdybayeva
National Center for Biotechnology, Korgalzhyn road, 13/5,Nur-Sultan , 010000, Kazakhstan
D.D. Rakhimbayeva
National Center for Biotechnology, Korgalzhyn road, 13/5,Nur-Sultan , 010000, Kazakhstan
A.A. Kakimzhanova
National Center for Biotechnology, Korgalzhyn road, 13/5,Nur-Sultan , 010000, Kazakhstan
Abstract
The expansion of exports and imports of potato and tomato crops between countries contributes to the rapid spread of the most harmful species and strains of phytopathogenic fungi around the world. Due to it, the identification of phytopathogenic fungi is important for agriculture. Currently, molecular-genetic methods are considered as one of the modern and promising methods for the diagnosis and identification of plant diseases, which allow identifying microorganisms without any knowledge of its biological features. One of the recommended methods for identification and classification of taxa is the determination of the nucleotide sequence of nuclear ribosomal gene regions.
276 infected potato tubers and tomato fruits were used to identify phytopathogenic fungi isolates, which were collected from production storages. Molecular-genetic identification of microorganisms based on the analysis of the nucleotide sequences of the ITS region made it possible to taxonomically identify 102 isolates of phytopathogenic fungi that belong to 7 genera. It was revealed that the proportion of fungi, which belohg to the genera Fusarium (60%), Penicillium (15%) and Clonostachys (12%), Alternaria (9%) were dominated in the generic structure.
The isolation frequency (Fr) and relative density (RD) of 7 generaphytopathogenic fungi were calculated according to the results of molecular-genetic identification.Isolation frequency (Fr)of fungi genera ranged from 0.3% to 22.1%. Relative density of fungi species ranged varied 0.9% to 59.8%.
Thereby, molecular-genetic identification based on the analysis of the nucleotide sequence of the ITS region made it possible to perform identification of pathogens on potato and tomato under production storage.Also, fungi Clonostachys and Acrostalagmus genera were isolated, which are not pathogens of potato and tomato diseases.
Keywords
Potato, tomato, fungi, ITS region, identification, isolation frequency, isolation relative density
Article Details
References
FAO (2017). Available at: URL (accessed on August 2017).
Kazakhstan’s Exports Continue to Grow and Diversify (2019) Available at: URL (accessed Febrary 1).
Food for Thought Politics within Human Nourishment (2017). Available at: URL (accessed June 2017).
Aringazina A., Gulis G., Allegrante J.P. Public Health Challenges and Priorities for Kazakhstan.Central Asian Journal, 2012, no.1, doi:10.5195/cajgh.2012.5 URL.
Tokbergenova A., Kiyassova L., Kairova S. Sustainable Development Agriculture in the Republic of Kazakhstan. Polish Journal of Environmental Studies, 2018, vol. 27, no. 5, pp.1923-1933.doi: org/10.15244/pjoes/78617.
Policy support is needed to develop agricultural co-operatives in Kazakhstan (Private sector development policy) Available at: URL competitiveness-programme/central-asia/Strengthening-agri-cooperatives-in-KZ.pdf (accessed 15 June 2017).
Kazakhstan’s economy grows 4.1% in five months, economy minister says Available at: URL April 2015).
Information Source for the global Potato Industry Available at: URL.
Agarwal S., Rao A.K. Tomato lycopene and its role in human health and chronic diseases. CMAJ, 2000, Vol. 163(6), pp. 739-744.
Singh V.K., Singh A.K., Kumar A. Disease management of tomato through PGPB: current trends and future perspective. 3 Biotech, 2017, Vol. 7(4), p. 255.doi: 10.1007/s13205-017-0896-1.
Kazenas L.D. Bolezni selskohozyaystvennyih rasteniy Kazahstana. Alma-Ata, Kaynar, 1993, 221 p.
The data set "Tomatoes, yield (hectogram per hectare)" for Kazakhstan contains data from the year 1992 until 2017 Available at: URL.
Obzor ryinka kartofelya v Kazahstane Available at: URL (Accessed May 2017).
A New Way to Fight Crop Diseases, With a Smartphone. A hand-held device could help farmers identify blighted plants, and perhaps reduce agricultural losses. It’s like a strep test for tomatoes and tubers. (2019) Available at: URL (accessed July 30).
GilJ.G.R., Tamayo P.J., Morales J.G. Identification and pathogenicity of microorganisms affecting purple passion fruit in Colombia. Rev. Ceres, 2017, vol. 64 no. 3, pp. 250-257. doi: 10.1590/0034-737X201764030005.
Tsui C.K.M., Woodhall J., Chen W., Lévesque C. A., Lau A., Schoen C.D., Baschien C., Najafzadeh M.J., Sybren de Hoog G. Molecular techniques for pathogen identification and fungus detection in the environment. IMA Fungus, 2011,vol. 2(2), pp. 177-189. doi: 10.5598/imafungus.2011.02.02.09.
AslamS.,TahirA., Aslam M.F.,Alam M.W., Shedayi A.A., Sadia S. Recent advances in molecular techniques for the identification of phytopathogenic fungi – a mini review. Journal of Plant Interactions, 2017, vol. 12, pp. 493-504. doi.org/10.1080/17429145.2017.1397205.
Pryce T.M., Palladino S., Kay I.D., Coombs G.W. Rapid identification of fungi by sequencing the ITS l and ITS2 regions using an automated capillary electrophoresis system. Medical Mycology, 2003, vol.41, pp. 369-381.
Schocha C.L., Seifertb K.A., Huhndorfc S., Robertd V., Spougea J.L., Levesqueb C.A., Chenb W., Fungal Barcoding Consortiuma. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi.Proc Natl AcadSci USA, 2012, vol. 109, no.16, 6241-6 p. doi:10.1073/pnas.1117018109.
Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical biochemistry, 1987, Vol. 162, no. 1. pp. 156-159.
Gonzalez H.H., Resnik S.L., Boca R.T., Marasas W.F. Mycoflora of Argentinian corn harvested in the main production area in 1990. Mycopathologia, 1995, vol. 130, no. 1, pp. 29-36.
Pacin A.M., Gonzalez H. H. L., Etcheverry M., Resnik S. L., Vivas L., Espin S. Fungi associated with food and feed commodities from Ecuador. Mycopathologia, 2003, vol. 156, no. 2, pp. 87-92. doi:org/10.1023/A:102294130.
Saleemi M.K., Khan M.Z., Khan A., Javed I. Mycoflora of poultry feeds and mycotoxins producing potential of Aspergillus species. Pakistan Journal of Botany, 2010, vol. 42, no. 1, pp. 427-434.
Ibrahim M., ShehuK., Sambo S., Tukur U., Umar I.A., Tafinta I.Y. Identification of fungi associated with storage rots of Irish Potato (Solanum Tuberosum l.) tubers in Sokoto Metropolis. Annals of Biological Sciences, 2014, vol. 2(2), pp. 1-4.
Kazerooni A.E., Maharachchikumbura S.S.N., Rethinasamy V., Al-Mahrouqi H., Al-Sadi A.V. Fungal Diversity in Tomato Rhizosphere Soil under Conventional and Desert Farming Systems. Front. Microbiol., 2017, vol. 8, 1462 p. doi: 10.3389/fmicb.2017.01462.
Egorova L.N., Kovaleva G.V. The structure of microfungi communities in natural and anthropogenic brown forest soils of Murav'ev-Amurskii peninsula (SouthernPrimorie). Mikologiya i Fitopatologiya, 2011, vol. 45, no. 2, pp. 125-133.
Fiers M., Chatot C., Edel-Hermann V., Le Hingrat Y., YanougoKonate A., Gautheron N., Guillery E., Alabouvette C., Steinberg C. Diversity of microorganisms associated with atypical superficial blemishes of potato tubers and pathogenicity assessment. European Journal of Plant Pathology, 2010, vol. 128 (3), pp. 353-371.doi: 10.1007/s10658-010-9657-2.
Stefańczyk E., Sobkowiak S., Brylińska M., Śliwka J. Diversity of Fusarium spp. associated with dry rot of potato tubers in Poland. European Journal of Plant Pathology, 2016, vol. 145 (4), pp. 871-884. doi: 10.1007/s10658-016-0875-0.