SPECIES COMPOSITION OF WOLF (CANIS LUPUS) HELMINTH FAUNA IN KAZAKHSTAN
Main Article Content
Authors
R. Uakhit
Saken Seifullin Kazakh Agrotechnical University, Nur-Sultan, Kazakhstan 010011
A. Smagulova
Saken Seifullin Kazakh Agrotechnical University, Nur-Sultan, Kazakhstan 010011
L. Lider
Saken Seifullin Kazakh Agrotechnical University, Nur-Sultan, Kazakhstan 010011
S. Leont'ev
Novosibirsk State Agrarian University, Novosibirsk, Russia, 630039
A. Berber
Consortium of hunting, tourist and fishing farms "Adal Zher", Temirtau, Kazakhstan, 101400
V. Kiyan
Saken Seifullin Kazakh Agrotechnical University, Nur-Sultan, Kazakhstan 010011
Abstract
The article presents data on the study of the species composition of wolf helminth fauna in the period from 2019 to 2021. A total of 39 wolves (Canis lupus) were examined, and their parasite fauna was assessed. The study identified the following types of helminths Echinococcus granulosus, Taenia krabbei, Dypilidium spp., Mesocestoides spp., Toxascaris leonina, Trichinella nativa, Dirofilaria repens. The extensiveness of the invasion wolves by helminths was relatively high in the western part region (96.5%), and towards the north-central part region, this indicator significantly decreased (65.2%). The average number of helminths was high, with some infected animals carrying several types of parasites. Thus, the intensity of invasion by helminths in wolves was 7.6 specimens per infected individual host. The research aimed to study the endoparasitic fauna of wild wolves using complex classical parasitological and molecular genetic methods. Samples of helminths were differentiated by amplification and sequencing using the marker gene cytochrome oxidase COX1 (GenBank accession number: MT877205, MZ505444, MZ669895, MZ724175). There is a need to assess the distribution of helminths in intermediate and final hosts populations and provide appropriate health education to avoid maintaining parasite life cycles and infestations, which will become a public health problem in the future.
Keywords
helminth fauna, molecular genetic analysis, identification, invasion
Article Details
References
Bajtursinov K.K. Faktory formirovaniya obshchnosti gel'mintofauny dikih i domashnih kopytnyh Kazahstana // Rossijskij parazitologicheskij zhurnal. – 2008. – № 4. – S. 5-12. [in Russian]
Sulejmenov M.ZH., Abdybekova A.M., Tlepov A.A., Tuganbaev A., Dzhusupbekova N.M. Rasprostranenie vozbuditelej parazitarnyh zoonozov v Kazahstane // Teoriya i praktika bor'by s parazitarnymi boleznyami, 2014, №15. – S. 296-298. [in Russian]
Biologicheskoe obosnovanie po regulirovaniyu chislennosti volka na territorii respubliki Kazahstan na period s 01.01.2020 g. po 31.12.2020 g. ot oktyabrya 2019 goda / Respubl. obshchestv. ob"edinenie «Associaciya sohraneniya bioraznoobraziya Kazahstana». – Alma-Ata, 2019. [in Russian]
Bizhanova N.A., Grachev YU.A., Saparov K.A., Grachev A.A., Rasprostranenie, chislennost' i nekotorye osobennosti ekologii krupnyh hishchnyh mlekopitayushchih v Kazahstane: analiticheskij obzor / Vestnik. Seriya ekologicheskaya. №3 (52), 2017. – S. 96-111. [in Russian]
SHestoj Nacional'nyj doklad Respubliki Kazahstan o biologicheskom raznoobrazii. Informacionno-analiticheskij centr ohrany okruzhayushchej sredy. Astana, 2018. [in Russian]
SHabdarbaeva, G.S., Abdybekova, A.M., SHapieva, ZH.ZH., Antropozoonozy i mery ih profilaktiki v Respublike Kazahstan, Almaty, 2012. – C. 104. [in Russian]
Leont'ev S.V., Lider L.A. O zarazhennosti stepnogo volka (Canis lupus campestris, Dwigubsci, 1804) kishechnymi gel'mintami v Central'nom Kazahstane // Vestnik NGAU, №1 (46), 2018. – S. 80-87. [in Russian]
Craig HL, Craig PS. Helminth parasites of wolves (Canis lupus): a species list and an analysis of published prevalence studies in Nearctic and Palaearctic populations // JHelminthol. 2005 Jun;79(2):95-103.
Hermosilla C, Kleinertz S, Silva LM, Hirzmann J, Huber D, Kusak J, Taubert A. Protozoan and helminth parasite fauna of free-living Croatian wild wolves (Canis lupus) analyzed by scat collection // Vet.Parasitol. 2017 Jan 15;233:14-19.
Bindke J.D, Springer A, Böer M, Strube C. Helminth Faunain Captive European Gray Wolves (Canis lupus lupus) in Germany // Front Vet Sci. 2017, Vol.4. – P. 228.
Molnar B, Ciucci P, Mastrantonio G, Betschart B. Correlates of parasites and pseudo parasites in wolves (Canis lupus) across continents: A comparison among Yellowstone (USA), Abruzzo (IT) and Mercantour (FR) national parks // Int J Parasitol Parasites Wildl. 2019;10:196-206.
Erol U, Danyer E, Sarimehmetoglu HO, Utuk AE. First Parasitological Data on a Wild Grey Wolf in Turkey with Morphological and Molecular Confirmation of the Parasites // Acta Parasitol. 2021 Jun;66(2):687-692.
Maslennikova O.V. K gel'mintofaune volka (Canis Lupus L.) Evropejskogo severo-vostoka Rossii // Sovremennye problemy prirodopol'zovaniya, ohotovedeniya i zverovodstva, 2012. – S. 360-361. [in Russian]
Fataliev G.G. Gel'mintofauna dikih psovyh v Azerbajdzhane i puti ee formirovaniya // Parazitologiya, 2011. – S. 129-139. [in Russian]
Bondarev A. YA., Kravchenko I. A., Boranbaev A. V. O gel'mintozah volka yugo-zapada Kulundy // Vestnik AGAU, 2008. №8 (46). – S. 43-45. [in Russian]
SHajkenov B.SH. Landshaftnye osobennosti formirovanie ochagov trihinelleza v Kazahstane // Izvestiya NAN RK, 2007, seriya biologicheskaya №3. – С. 57-65. [in Russian]
Abdybekova A. M., Torgerson P. R. Frequency distributions of helminths of wolves in Kazakhstan //Veterinary Parasitology, 2012. – Vol. 184. – №. 2-4. – P. 348-351. [in Russian]
Lider L.A., Akibekov O.S., Erzhanova N.S., Rasprostranenie trihinelleza sredi dikih plotoyadnyh v nekotoryh regionah Respubliki Kazahstana // «Sovremennye aspekty innovacii v sel'skohozyajstvennom proizvodstve YUgo-Zapadnogo regiona Kazahstana» Materialy mezhdunarodnoj nauchno-prakticheskoj konferencii posvyashchennoj pamyati doktora sel'skohozyajstvennyh nauk, professrora Tuekbasova M.K., 332-336. [in Russian]
Skryabin K.I. Metod polnyh gel'mintologicheskih vskrytii pozvonochnyh, vklyuchaya cheloveka. g.: Moskva, – 1928. – S. 40-48. [in Russian]
Movsesyan S.O., Protasova E.N., Skryabin K.I.. Metody polnyh gel'mintologicheskih vskrytij pozvonochnyh zhivotnyh, vklyuchaya cheloveka. Tovarishchestvo nauchnyh izdanij KMK, Nauchnye trudy, tom 2. – М., – 2018. – С. 10-15. [in Russian]
Kapustin V.F. Atlas naibolee rasprostranennyh gel'mintov sel'skohozyajstvennyh zhivotnyh. – M.: Sel'hozgiz, – 1953. – С. 20-140. [in Russian]
Kozlov D.P. Opredelitel' gel'mintov hishchnyh mlekopitayushchih SSSR. – M.: Nauka, 1977. – С. 10-200. [in Russian]
Anisimova E.I, Poloz S.V., Subbotin A.M. Gel'minty hishchnyh mlekopitayushchih (semejstvo Canidae, Fischer, 1817) v estestvennyh usloviyah i na zverofermah. // Belorusskaya nauka, – 2011. – С. 31-150. [in Russian]
Wallace Peters, Docteur Honoris Causa, Geoffrey Pasvol, Atlas of tropical medicine and Parasitology // Elsevier Limited, – 2007. – Р. 21-385.
Myriam Consuelo Lopez. Atlas de parasitologia. Мanual modern, – 2006. – Р. 10-125.
Trachsel D., Deplazes P., Mathis A. Identification of taeniid eggs in the faeces from carnivores based on multiplex PCR using targets in mitochondrial DNA // Parasitology, 2007, Vol. 134. – P. 911-920.
Saitou N. and Nei M. The neighbor-joining method: A new method for reconstructing phylogenetic trees // Molecular Biology and Evolution, 1987. Vol.4:406-425.
Tamura K., Nei M., and Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method // Proceedings of the National Academy of Sciences (USA), 2004. Vol.101:11030-11035.
Kumar S., Stecher G., Li M., Knyaz C., and Tamura K. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms // Molecular Biology and Evolution, 2018. Vol. 35:1547-1549
Uakhit RS, Lider LA, Leontyev SV, Abdrakhmanov SK, Kiyan VS First molecular identification of Dirofilaria repens found in a wolf heart in Kazakhstan // Adv.Anim.Vet. Sci, 2021, 9 (9): 1342-1346.