Obtaining Multiprotein Antigens Of Brucella And Study Of Their Immunoreactivity
Main Article Content
Authors
B.K. Ingirbai
S. Seifullin Kazakh Agro Technical University, Zhenis avenue, 62, Nur-Sultan, 010011, Kazakhstan
A. Syzdykova
S. Seifullin Kazakh Agro Technical University, Zhenis avenue, 62, Nur-Sultan, 010011, Kazakhstan
A.K. Kurmasheva
S. Seifullin Kazakh Agro Technical University, Zhenis avenue, 62, Nur-Sultan, 010011, Kazakhstan
A.K. Bulashev
S. Seifullin Kazakh Agro Technical University, Zhenis avenue, 62, Nur-Sultan, 010011, Kazakhstan
Abstract
Rose-Bengal test, complement fixation test and the agglutination test are mainly used for the diagnosis of animal brucellosis. These tests are characterized by low sensitivity and specificity, which is one of the main reasons for the low effectiveness of measures aimed at eradicating brucellosis. The use of modern highly sensitive serological tests requires the availability of antigens specific to Brucella spp. The aim of the study was to obtain multiproteins of the pathogen by recombinant DNA technology and to study their antigenic properties. In the course of the study, three types of multiproteins were obtained, constructed from diagnostically important peptides that form B.abortus and B.melitensis outer membrane proteins. All target products were synthesized by the producer strain in a form that is authentic to natural proteins, and showed immunogenicity in mouse model. Antibodies produced against the multiproteins were specific for the single proteins of the pathogen's cell wall. The data obtained indicate the need to continue studies to determine the possibility of using multiproteins as an antigen in an enzyme-linked immunosorbent assay to detect anti-Brucella specific antibodies.
Keywords
Brucella spp., outer membrane protein, antigen, serological diagnostics
Article Details
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