MODULATION OF HELICOBACTER PYLORI TRANSCRIPTIONAL PROFILE BY SUBINHIBITORY CONCENTRATIONS OF RIFAMPICIN
Main Article Content
Authors
Kuvat Momynaliev
National Center for Biotechnology, Sh. Valikhanov str. 13/1, Astana, Kazakhstan
Vera Chelysheva
Research Institute for Physico-Chemical Medicine, Malaya Pirogovskaya 1A, Moscow, Russia
Oksana Selezneva
Research Institute for Physico-Chemical Medicine, Malaya Pirogovskaya 1A, Moscow, Russia
Andrey Larin
Research Institute for Physico-Chemical Medicine, Malaya Pirogovskaya 1A, Moscow, Russia
Tatyana Akopian
Research Institute for Physico-Chemical Medicine, Malaya Pirogovskaya 1A, Moscow, Russia
Dmitry Alexeev
Research Institute for Physico-Chemical Medicine, Malaya Pirogovskaya 1A, Moscow, Russia
Veronique Le Berre
Biochips platform of Genopole, University of Toulouse, INSA, UPS, INP & INRA, 135, Avenue de Rangeuil, F-31077, Toulouse
Serguei Sokol
Biochips platform of Genopole, University of Toulouse, INSA, UPS, INP & INRA, 135, Avenue de Rangeuil, F-31077, Toulouse
Jean-Marie Francois
Biochips platform of Genopole, University of Toulouse, INSA, UPS, INP & INRA, 135, Avenue de Rangeuil, F-31077, Toulouse
Vadim Govorun
Research Institute for Physico-Chemical Medicine, Malaya Pirogovskaya 1A, Moscow, Russia
Abstract
Subinhibitory concentrations (sub - MICs) of antibiotics do not kill bacteria, but they are able to interfere with important aspects of bacterial cell function, such as adhesion to host cells, surface bacterial energy, susceptibility to host defense mechanisms, inhibition of enzyme function and toxin production. In order to understand how H. pylori copes with environmental stress and what facilitates the emergence of RIF mutants in H. pylori, we used DNA microarrays to compare the gene expression profiles of H. pylori in the presence and absence of subinhibitory concentrations of rifampicin (1/16 MIC (0.1 mg/L), 1/8 MIC (0,2mg/L), ¼ MIC (0,4 mg/L), and ½ MIC (0,8 mg/L). We found that еhe expression of 57 genes (of the 1,576 genes analyzed) was increased more than ≥ 1,5 – fold, and the expression of only 29 genes was decreased more than ≤ 1,5 –fold in significant way (p-value < 0,05), when H. pylori was treated with sub – MICs of RIF. No correlation was found between the sub - MICs of RIF and gene expression. We conclude that the alteration in the transcriptional pattern of H. pylori after the exposure to sub – MICs of RIF is mainly due to a direct interaction between rifampicin and the RNA polymerase β-subunit. Finally, we propose that subinhibitory concentrations of rifampicin may lead to an increase in the number of hypermutable cells in the H. pylori population.
Keywords
Helicobacter pylori, selection, microarray, rpoB
Article Details
References
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