Evaluation Of The Mutagenic Potential Of The Polyphenol Extract From Blueberries In The Ames Test
Main Article Content
Authors
Z.T. Shulgau
National Center for Biotechnology, 13/5, Kurgalzhynskoye road, Nur-Sultan,010000, Kazakhstan
S.D. Sergazy
National Center for Biotechnology, 13/5, Kurgalzhynskoye road, Nur-Sultan,010000, Kazakhstan
A.M. Zhulikeeva
National Center for Biotechnology, 13/5, Kurgalzhynskoye road, Nur-Sultan,010000, Kazakhstan
A.Y. Dautov
National Center for Biotechnology, 13/5, Kurgalzhynskoye road, Nur-Sultan,010000, Kazakhstan
A.Y. Gulyayev
National Center for Biotechnology, 13/5, Kurgalzhynskoye road, Nur-Sultan,010000, Kazakhstan
L.V. Kovalenko
Surgut State University, 14, Energetikov st., Surgut, 628412, Russia
L.D. Belotserkovtseva
Surgut State University, 14, Energetikov st., Surgut, 628412, Russia
N.S. Kavushevskaya
Surgut State University, 14, Energetikov st., Surgut, 628412, Russia
A.V. Logutenko
Surgut State University, 14, Energetikov st., Surgut, 628412, Russia
Abstract
In this research, mutagenic properties of blueberry polyphenol extract were studied in gene mutation induction test (Ames test) on four strains of Salmonella typhimurium TA98, TA100, TA1535, TA1537. None of the strains of Salmonella typhimurium showed statistically reliable dose-dependent increase in number of revertant colonies in the presence of investigated drug in the studied dose range from 4,0 to 40,0 mg/ml relative to baseline of spontaneous mutations. The blueberry extract does not have any mutagenic activity in the researched dose range in relation to TA98, TA100, TA1535, TA1537 strains of Salmonella typhimurium.
Keywords
polyphenols, blueberry extract, Ames test, mutagenic properties
Article Details
References
Lätti A.K., Jaakola L., Riihinen K.R., Kainulainen P.S. Anthocyanin and flavonol variation in bog bilberries (Vaccinium uliginosum L.) in Finland. Journal of Agricultural and Food Chemistry, 2010, vol. 58, no. 1, pp. 427–433. 20000402, Crossref
Bonfigli M., Godoy E., Reinheimer M.A., Scenna N.J. Comparison between conventional and ultrasound-assisted techniques for extraction of anthocyanins from grape pomace. Experimental results and mathematical modeling. Journal of Food Engineering, 2017, vol. 207, pp. 56–72. Crossref
Duman A.D., Ozgen M., Dayisoylu K.S., Erbil N., Durgac C. Antimicrobial activity of six pomegranate (Punica granatum L.) varieties and their relation to some of their pomological and phytonutrient characteristics. Molecules, 2009, vol. 14, pp. 1808–1817. 19471201, Crossref
Giampieri F., Alvarez-Suarez J.M., Battino M. Strawberry and human health: Effects beyond antioxidant activity. Journal of Agricultural and Food Chemistry, 2014, vol. 62, pp. 3867–3876. 24450925, Crossref
Hu J., Webster D., Cao J., Shao A. The safety of green tea and green tea extract consumption in adults–results of a systematic review. Regulatory Toxicology and Pharmacology, 2018, vol. 95, pp. 412–433. 29580974, Crossref
Lee S., Song P.H., Lee Y.J., Ku S.-K., Song C.-H. Acute and subchronic oral toxicity of fermented green tea with aquilariae lignum in rodents. Evidence-Based Complementary and Alternative Medicine, 2019, vol. 2019, pp. 1-11. 31662782, Crossref
Mennen L.I., Walker R., Bennetau-Pelissero C., Scalbert A. Risks and safety of polyphenol consumption. The American Journal of Clinical Nutrition, 2005, vol. 81, pp. 326S–329S. 15640498, Crossref
Teschke R., Zhang L., Melzer L., Schulze J., Eickhoff A. Green tea extract and the risk of drug-induced liver injury. Expert Opinion on Drug Metabolism & Toxicology, 2014, vol. 10, pp. 1663–1676. 25316200, Crossref
Filippini T., Malavolti M., Borrelli F., Izzo A.A., Fairweather‐Tait S.J., Horneber M., Vinceti M. Green tea (Camellia sinensis) for the prevention of cancer. Cochrane Database of Systematic Reviews, 2020, vol. 1, pp. 1-274. 19588362, Crossref
Singleton V.L, Orthofer R., Lamuela-Raventós R.M. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 1999, vol. 299, pp. 152–178. Crossref
Ames B.N., McCann J., Yamasaki E. Methods for Detecting Carcinogens and Mutagens with the Salmonella/Mammalian-Microsome Mutagenicity. Mutation Research/Environmental Mutagenesis and Related Subjects, 1975, vol. 31, pp. 347-364. 768755, Crossref
Maron D.M., Ames B.N. Revised Methods for the Salmonella Mutagenicity Test. Mutation Research/Environmental Mutagenesis and Related Subjects, 1983, vol. 113, pp. 173-215. 6341825, Crossref
Gatehouse D., Haworth S., Cebula T., Gocke E., Kier L., Matsushima T., Melcion C., Nohmi T., Venitt S., Zeiger E. Recommendations for the Performance of Bacterial Mutation Assays. Mutation Research/Environmental Mutagenesis and Related Subjects, 1994, vol. 312, pp. 217-233. 7514736, Crossref
Flückiger-Isler S., Kamber M. Direct comparison of the Ames microplate format (MPF) test in liquid medium with the standard Ames pre-incubation assay on agar plates by use of equivocal to weakly positive test compounds. Mutation Research/Environmental Mutagenesis and Related Subjects, 2012, vol. 747, no. 1, pp. 36-45. 22579797, Crossref
Kamber M., Flückiger-Isler S., Engelhardt G., Jaeckh R., Zeiger E. Comparison of the Ames II and traditional Ames test responses with respect to mutagenicity, strain specificities, need for metabolism and correlation with rodent carcinogenicity. Mutagenesis, 2009, vol. 24, no. 4, pp. 359-366. 19447896, Crossref
Zeiger E. The test that changed the world: The Ames test and the regulation of chemicals. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2019, vol. 841, pp. 43–48. 31138410, Crossref
Vijay U., Gupta S., Mathur P., Suravajhala P., Bhatnagar P. Microbial Mutagenicity Assay: Ames Test. Bio-Protocol, 2018, vol. 8, no. 16, pp. 1-15. 34179285, Crossref