APPLICATION OF THE IMMUNOCHROMATOGRAPHIC TEST KIT FOR SEROLOGICAL DIAGNOSIS OF BRUCELLOSIS BASED ON RECOMBINANT ANTIGEN

Main Article Content

Authors

Е. Manat

National center for biotechnology, 13/1, Sh. Valikhanovst., Astana, 010000, Kazakhstan

N.I. Sarina

National center for biotechnology, 13/1, Sh. Valikhanovst., Astana, 010000, Kazakhstan

S.Z. Eskendirova

National center for biotechnology, 13/1, Sh. Valikhanovst., Astana, 010000, Kazakhstan

A.V. Shustov

National center for biotechnology, 13/1, Sh. Valikhanovst., Astana, 010000, Kazakhstan

K.K. Mukanov

National center for biotechnology, 13/1, Sh. Valikhanovst., Astana, 010000, Kazakhstan

Abstract

Despite the significant progress jointly made by scientists and practical veterinary service in the Republic of Kazakhstan, the problem of animal brucellosis has not been completely solved. Consistently high levels of human brucellosis morbidity, which is due to extremely tense epizootic situation and large socio-economic damage, determine the special importance of this infection in the structure of infectious pathologies. One of the potential to increase the sensitivity and specificity of serological diagnostic methods of brucellosis is the use of recombinant analogs of immunodominant proteins of pathogenic Brucella, which diversity has determined and studied at the present time. Periplasmic protein BP26 is a specific antigen which is highly conserved for genus of Brucella. It has high diagnostic value for use in the development of immunoassays and immunochromatographic test kits of brucellosis for the veterinary and medical use.

A highly sensitive and specific immunoassay system for rapid detection of antibrucellar antibodies was developed on the basis of recombinant Brucella antigen BP2. It is intended for serological diagnosis of brucellosis in animals. High diagnostic efficiency of ICA was tested in 962 samples of blood serum of cattle and goats, sheep as well as 16 control reference sera.

Keywords

immunochromatographic assays, recombinant antigen, blood serum, antibody

Article Details

References

Poester F.P., Nielsen K., Samartino L.E., Yu W. Diagnosis of Brucellosis.The Open Veterinary Science Journal, 2010,vol.4, pp. 46-60.

Smirnova E., Vasin A., Sandybaev N.T. et al. Current Methods of Human and Animal Brucellosis Diagnostics.Advances in Infectious Diseases, 2013, vol. 3,pp. 177-184.

Supriya C., Umapathy B., Ravikumar K. Brucellosis: rewiew on the recent trends in pathogenicity and laboratory. Journal of Laboratory Physicians, 2010, vol. 2,pp. 55-62.

Oliveira S.C., Macedo G.C., Almedia L.A. et al. Recent Advances in Understanding Immunity Against Brucellosis: Application for Vaccine Development. The Open Veterinary Science Journal, 2010, vol. 4, pp. 102-108.

Moryon I., Lopez-Goni I. Structure and properties of the outer membranes of Brucella abortus and Brucella melitensis. InternatlMicrobiol, 1998, vol. 1, pp. 19-26.

Dahouk S.A., Nockler K., Scholz H.C. et al. Immunoproteomic characterization of Brucella abortus 1119-3 preparations used for the serodiagnosis of Brucella infections. Journal of Immunological Methods, 2006, vol. 309, pp. 34-47.

Ko K.Y., Kim J.W., Her M. et al. Immunogenic proteins of Brucella abortus to minimize cross reactions in brucellosis diagnosis.Veterinary Microbiology, 2012, vol. 156, pp. 374-380.

Winter A. Outer membrane proteins of Brucella.Ann. Inst. Pasteur Microbiol., 1987,vol. 138, pp. 87-90.

Whatmore A.M. Current understanding of the genetic diversity of Brucella, an expanding genus of zoonotic pathogens. Infect. Genet.Evol.,2009, vol. 9, pp. 1168-1184.

Dahouk S., Hofer E., Tomaso H. et al. Intraspecies Biodiversity of the Genetically Homologous Species Brucella.Appl Environ Microbiol.,2012, vol. 78, no. 3, pp. 1534-1543.

Cassataro J., Pasquevich K., Bruno L. et al. Antibody Reactivity to Omp31 from Brucella melitensis in Human and Animal Infections by Smooth and Rough Brucellae.Clinical and Diagnostic Laboratory Immunology, 2004, vol. 11, no. 1,pp. 111-114.

Matar G.M., Khneisser I.A., Abdelnoor A.M. Rapid Laboratory Confirmation of Human Brucellosis by PCR Analysis of a Target Sequence on the 31-Kilodalton Brucella Antigen DNA.Journal of Clinical Microbiology, 1996, vol. 34, no. 2,pp. 477-478.

Ficht T., Bearden S., Sowa B., Adams L. DNA sequence and expression of the 36-kilodalton outer membrane protein gene of Brucella abortus.Infection and Immunity,1989, vol.57, no. 11,pp.3281-3291.

Bricker B.J., Tabatabai L.B., Judge B.A. et al. Cloning, Expression, and Occurrence of the Brucella Cu-Zn Superoxide Dismutase.Infection and Immunity, 1990, vol. 58, no. 9,pp. 2935-2939.

Tabatabai L.B., Hennager S.G. Cattle Serologically Positive for Brucella abortus Have Antibodies to B. abortus Cu-Zn Superoxide Dismutase.Clinical and Vaccine Immynology, 1994, vol. 1, no. 5, pp. 506-510.

Onate A., Cespedes S., Cabrera A.et al. A DNA vaccine encoding Cu-Zn Superoxide Dismutase of Brucella abortus induced protective immunity in BALB/c mice.Infection and Immunity, 2003, vol. 71, no. 3,pp. 4857-4861.

Mayfield J., Tabatabai L. Recombinant Brucella abortus gene expressing immunogenic protein. United States Patent, no. 5023174, 1991.

Kazak E., Oliveira S.C., Goral G. et al. Brucella abortusL7/L12 Recombinant protein Induces Strong Th1 Response in Acute Brucellosis Patients.Iranian journal of immunology,2010, vol. 7, no. 3,pp. 132-141.

Luo D., Ni B., Li P. et al. Protective Immunity Elicited by a Divalend DNA Vaccine Encoding Both the L7/L12 and Omp16 Genes of Brucella abortus in BALB/c Mice.Infection and Immunity, 2006, vol. 74, no. 5,pp. 2734-2741.

Сloeckaert A., Baucheron S., Vizcaino N., Zygmunt M.S. Use of Recombinant BP26 protein in Serological Diagnosis of Brucella melitensis infection in Sheep.Clinical and Diagnostic Laboratory Immunology, 2001, vol. 8,no. 4,pp. 772-775.

Gupta V.K.,Kumari R., Vohra J. et al. Comparative evaluation of recombinant BP26 protein for serological diagnosis of Brucella melitensis infection in goat. Small Ruminant Research,2010, vol. 93,pp. 119-125.

Xin T., Yang H., Wang N. et al. Limitations of the BP26 Protein-Based Indirect Enzyme-Linked Immunosorbent Assay for Diagnosis of Brucellosis. Clinical and vaccine immunology,2013, vol. 20,no. 9,pp. 1410-1417.

Seco-Mediavilla P., Verger J.M., Grayon M. et al. Epitope mapping of the Brucella melitensis BP26 immunogenic protein: usefulness for diagnosis of sheep brucellosis.Clinical and diagnostic laboratory immunology, 2003, vol. 10, pp. 647-651.

Cloeckaert A., Baucheron S., Vizcaino N., Zygmunt M. Use of recombinant BP26 protein in serological diagnosis of Brucella melitensis infection in sheep.Clinical and diagnostic laboratory immunology, 2001, vol.8,pp. 772-775.

Liu W., Hu S., Qiao Z. et al. Expression, purification and improved antigenic specificity of a truncated recombinant BP26 protein of Brucella melitensis M5-90: a potencial antigen for differencialserodiagnosis of brucellosis in sheep and goats.Biotechnol. Appl. Biochem., 2011, vol.58,pp. 32-38.

Lim J., Kim D., Lee J. et al. Evaluation of recombinant 28kDa outer membrane protein of Brucella abortus for the clinical diagnosis of bovine brucellosis in Korea.J. Vet. Med. Sci., 2012, vol. 74,pp. 687-691.

Thavaselvam D., Kumar A., Tiwari S. et al. Cloning and expression of the immunoreactiveBrucella melitensis 28 kDa outer-membrane protein (Omp28) encoding gene and evaluation of the potential of Omp28 for clinical diagnosis of brucellosis. Journal of Medical Microbiology,2010, vol. 59,no. 4,pp. 421-428.

Qiu J., Wang W., Wu J., Zhang H. Characterization of periplasmic protein BP26 epitopes of Brucella melitensis reacting with murine monoclonal and sheep antibodies.PLoS ONE, 2012, no. 7(3), pp.e34226. doi:10.1371.

Kumar S.,Tuteja U., Kumar A., Batra H. Expression and purification of the 26kDa periplasmic protein of Brucella abortus a reagent for the diagnosis of bovine brucellosis.Biotechnol. Appl. Biochem., 2008, no. 49, pp. 213-218. doi 10.1042/BA20070.

Hosseini S., Azizpour M., Akbari N., Basiri H. Amplification,cloning and expression of Brucella melitensisbp 26 gene (OMP28) isolated from Marcazi and purification of Bp26 protein.Archives of Razi Institute, 2013, vol. 68,no. 2,pp. 111-116.

Kim D., Park J., Kim S., Sox Y. Brucella Immunogenic BP26 Forms aChannel-like Structure.J. Mol. Biol., 2013, no. 425, pp. 1119-1126. doi.org/10.1016/j.jmb.2013.01.015.

Frens G. Controlled nucleation for the regulation of the particle size in monodiperse gold suspensions.Nat. Phys. Sci,1973,vol. 241,20 р.

Hermanson G.T. Preparation of colloidal-gold-labelled-proteins.London. Bioconjugate Techniques.Academic Press, 1996,593 р.

Bradford M.A. A rapid and sensitive method fer the qvantitation of microgram qvantitaties of protein utilizing the principle of protein – due binding.Anal.biochem.,1976, vol. 72, no. 4, pp. 248-254.

Koichubekov B.K., Sorokina M.A., Bukeeva А.О. Biostatistics in examples and problems. – Almaty, 2012. – 80 p.