IN VITRO CULTURE OF Rhodiola rosea L.
Main Article Content
Authors
O.N. Khapilina
National Center for Biotechnology, Kurgalzhyn road, 13/5, Astana, 010000, Kazakhstan
Z.S. Kupeshev
National Center for Biotechnology, Kurgalzhyn road, 13/5, Astana, 010000, Kazakhstan
A.N. Danilova
National Center for Biotechnology, Kurgalzhyn road, 13/5, Astana, 010000, Kazakhstan
R.N. Kalendar
National Center for Biotechnology, Kurgalzhyn road, 13/5, Astana, 010000, Kazakhstan
Abstract
We present the results of our study on in vitro culture of the valuable medicinal plant Rhodiola rosea (Rhodiola rosea L.). We collected sample plant materials from the Kazakh Altai territory. The essential stages of in vitro cultivation techniques for R. rosea were optimized. We determined the most effective sterilization protocol for different types of explants of Rhodiola and optimized stages of organogenesis and formation of adventitious shoots. We found that aseptic cultures of Rhodiola necessitate the use of multi-stage sterilization protocol using different types of antiseptics. Apexes of shoots and rhizome buds were the most effective explants. High concentration of exogenous cytokinin in the culture medium induced adventitious organogenesis in R. rosea. Maximum adventitious shoots (up to 34 explants) were observed usingMSZ1 medium supplemented with zeatin, indole-3-acetic acid, and gibberellic acidat concentrations of 2, 0.1, and 0.5 mg/L, respectively. The formation of the root system wasdependent on the agar concentration in the medium. Morphometric characteristics were higher in regenerated Rhodiolagrown on a medium containing 0.6% agar.
Keywords
golden root, Rhodiola rosea L., in vitro culture, explants, adventitious organogenesis, micropropagation
Article Details
References
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