MOLECULAR GENETIC ANALYSIS OF MICRODELETIONS OF Y-CHROMOSOME AZF LOCUS
Main Article Content
Authors
A.D. Kairzhanova
National Center for Biotechnology, 13/5, Korgalzhyn road, Astana, 010000, Kazakhstan
D.K. Kamalova
National Center for Biotechnology, 13/5, Korgalzhyn road, Astana, 010000, Kazakhstan
G.D. Abisheva
National Center for Biotechnology, 13/5, Korgalzhyn road, Astana, 010000, Kazakhstan
A.O. Amirgazin
National Center for Biotechnology, 13/5, Korgalzhyn road, Astana, 010000, Kazakhstan
V.B. Shvedyuk
National Center for Biotechnology, 13/5, Korgalzhyn road, Astana, 010000, Kazakhstan
O.A. Popova
PLL «Astana ECOLIFE», 22, Alash road, Астана, 010000, Казахстан
G.M. Kim
PLL «Astana ECOLIFE», 22, Alash road, Астана, 010000, Казахстан
A.B. Shevtsov
National Center for Biotechnology, 13/5, Korgalzhyn road, Astana, 010000, Kazakhstan
Abstract
Male infertility plays a significant role in reproduction. Although medical therapy can successfully treat ejaculation disorder and impotence,a majority of genetic disorders remain therapeutically intractable. Y-chromosome deletions are the most common causes of male infertility. The distal end of the Y-chromosome long arm bears the locus of azoospermia factor (AZF), which carriesgenes essential for spermatogenesis. Microdeletions inthe Y-chromosome long arm are associated with spermatogenesis disorder and are the most common genetic cause of oligospermia(5-10%) and azoospermia(10-15%). In our study, we analysed microdeletions of the AZF locus, using sixSTS markers (recommended by the European Association of Andrology),two gene controls for reaction inhibition, and a Y-chromosome control.
We tested DNA samples of 138 male subjects with the developed testsystem. The distribution results of microdeletions of the Y-chromosome AZF locus were obtained for samples from the Kazakh population. Deletion of the AZF locus was reported in11% of males with oligospermia and 16% males (7 of 45 subjects)with azoospermia.
Keywords
Y-chromosome, AZF locus, STS markers, PCR, male infertility, microdeletion
Article Details
References
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