MODIFIED GEL BASED ON RECOMBINANT HUMAN ANGIOGENIN: STUDY OF ANTIMICROBIAL, ANTI-INFLAMMATORY AND WOUND HEALING EFFECTS
Main Article Content
Authors
Zh.T. Bokebayev
AO «Medical University Astana», Beibitshilik Ave., 49A, Astana, 010000, Kazakhstan
I.S. Kukiev
AO «Medical University Astana», Beibitshilik Ave., 49A, Astana, 010000, Kazakhstan
T.N. Krivoruchko
RSE«National Center for Biotechnology», Kurgalzhinskoe highway, 13/5, Astana, 010000, Kazakhstan
Sh.D. Sergazy
RSE«National Center for Biotechnology», Kurgalzhinskoe highway, 13/5, Astana, 010000, Kazakhstan
Z.T. Shulgau
RSE«National Center for Biotechnology», Kurgalzhinskoe highway, 13/5, Astana, 010000, Kazakhstan
O.V. Tolmacheva
RSE«National Center for Biotechnology», Kurgalzhinskoe highway, 13/5, Astana, 010000, Kazakhstan
G.T. Abuova
South-Kazakhstan State Pharmaceutical Academy, Al-Farabi, 1, Shymkent, 160019, Kazakhstan
S.K. Zhaugasheva
Karagandy State Medical University, Gogolya str., 40, Karaganda, 100000, Kazakhstan
A.E. Gulyayev
RSE«National Center for Biotechnology», Kurgalzhinskoe highway, 13/5, Astana, 010000, Kazakhstan
Abstract
The aim of the study was to compare the antimicrobial, anti-inflammatory and wound healing effects of an angiogenin gel with those of a modified version of the gel containing the antimicrobial drug dioxidine. Modifications of the angiogenin gel by the addition of dioxidine resulted in enhanced antimicrobial action against the main pathogens of wound purulent-septic infections: Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, and Escherichia coli. The presence of dioxidine in the angiogenin gel did not modify the anti-inflammatory effect, which was similar to that of the standard angiogenin gel. The wound healing effect of the angiogenin gel containing dioxidine was enhanced in a burn wound model, as accelerated epithelization was observed, potentially due to the contribution of the antimicrobial component to the healing process.
Keywords
Angiogenin, dioxidine, antimicrobial, anti-inflammatory, wound healing
Article Details
References
Riabov V., Salazar F., Htwe S.S.et al. Generation of anti-inflammatory macrophages for implants and regenerative medicine using self-standing release systems with a phenotype-fixing cytokine cocktail formulation. ActaBiomater, 2017,feb, 1,pii: S1742-7061(17)30080-6. [Epub ahead of print]. PMID: 28159717.doi: 10.1016/j.actbio.2017.01.071.
Sherman S.E., Kuljanin M., Cooper T.T.et al. High Aldehyde Dehydrogenase Activity Identifies a Subset of Human Mesenchymal Stromal Cells with Vascular Regenerative Potential. Stem Cells., 2017,mar, 15. [Epub ahead of print]. PMID: 28295901.doi: 10.1002/stem.2612.
Lv Y., Ge L., Zhao Y. Effect and mechanism of SHED on ulcer wound healing in Sprague-Dawley rat models with diabetic ulcer.Am J Transl Res., 2017,vol. 9(2), pp. 489-498. PMID: 28337277.
Chodorowska G., Roguś-Skorupska D. Cutaneous wound healing. Ann UnivMariae Curie Sklodowska Med.,2004,vol. 59(2), pp. 403-407. PMID: 16146117.
Sheng J., Xu Z. Three decades of research on angiogenin: a review and perspective.ActaBiochimBiophys Sin (Shanghai), 2016,vol. 48(5), pp. 399-410. PMID: 26705141.doi: 10.1093/abbs/gmv131.
Lyons S.M., Fay M.M., Akiyama Y., Anderson P.J., Ivanov P. RNA biology of angiogenin: Current state and perspectives.RNA Biol., 2017, vol. 14(2), pp.171-178. PMID: 28010172.doi: 10.1080/15476286.2016.1272746.
Hoang T.T., Raines R.T. Molecular basis for the autonomous promotion of cell proliferation by angiogenin. Acids Res., 2017, vol. 45(2),pp. 818-831. PMID: 27915233. PMCID: PMC5314776.doi: 10.1093/nar/gkw1192.
Li S., Hu G.F. Emerging role of angiogenin in stress response and cell survival under adverse conditions. J Cell Physiol., 2012,vol. 227(7),pp. 2822-2826. PMID: 22021078. PMCID: PMC3271170.doi: 10.1002/jcp.23051.
Simanski M., Köten B., Schröder J.M., Gläser R., Harder J. Antimicrobial RNases in cutaneous defense. J Innate Immun., 2012,vol. 4(3),pp. 241-247. PMID: 22327069.doi: 10.1159/000335029.
Navashin S.M. Racional'najaantibiotikoterapija[Rational antibiotic therapy]. Moscow, Medicina [Medicine], 1982, 496 p.
Gacura V.V. Metodypervichnogofarmakologicheskogoissledovanijabiologicheskiaktivnyhveshhestv [Methods of primary pharmacological research of biologically active substances]. Moscow, Medicina [Medicine], 1974, 123 p.
Burmistrov V.M., Kalmetov B.M. Mestnoekonservativnoelechenieozhogov [Local conservative treatment of burns].Ozhogi: Rukovodstvodljavrachej [Burns: Guide for doctors]. Leningrad,Medicina [Medicine], Leningr. otd., 1981, pp. 123-134.
Belen'kij M.L. Jelementykolichestvennojocenkifarmakologicheskogojeffekta [Elements of quantitative evaluation of the pharmacological effect], 1963, pp. 81-106.
Peculiarities of hepatic experimental wound healing in rats treated with angiogenin. Morfologiia, 2005,vol. 128(4),pp. 98-100.
Lee S.H., Kim K.W., Min K.M., Kim K.W., Chang S.I., Kim J.C. Angiogenin reduces immune inflammation via inhibition of TANK-binding kinase 1 expression in human corneal fibroblast cells. Mediators Inflamm., 2014,vol. 2014,pp. 861435.
Kendall A.C., Whatmore J.L., Harries L.W.et al. in inflammatory gene expression induced by hyperbaric oxygen treatment in human endothelial cells under chronic wound conditions. Exp Cell Res., 2012, feb 1, no. 318(3),pp. 207-216.
Koutroubakis I.E., Xidakis C., Karmiris K.et al. Serum angiogenin in inflammatory bowel disease. Dig Dis Sci., 2004,nov-dec, no. 49(11-12),pp. 1758-1762.
Eleftheriadis T., Antoniadi G., Liakopoulos V.et al. Plasma angiogenin and vascular endothelial growth factor a among hemodialysis patients. Iran J Kidney Dis., 2012,may, no. 6(3),pp. 209-215.
Ng M.T., Stammers A.T., Kwon B.K. Vascular disruption and the role of angiogenic proteins after spinal cord injury. Transl Stroke Res., 2011,dec,no. 2(4),pp. 474-491.
Pan S.C., Wu L.W., Chen C.L., Shieh S.J., Chiu H.Y. Angiogenin expression in burn blister fluid: implications for its role in burn wound neovascularization.Wound Repair Regen., 2012,vol. 20(5), pp. 731-739. PMID: 22823522.doi: 10.1111/j.1524-475X.2012.00819.x.
Steed D.L., Trumpower C., Duffy D., et al. Amnion-derived cellular cytokine solution: a physiological combination of cytokines for wound healing. Eplasty, 2008,apr 7, no. 8,pp. e18. PMID: 18461121. PMCID: PMC2311453.