OPTIMIZATION OF BARBERRY MICROPROPAGATION
Main Article Content
Authors
N.V. Romadanova
Institute of Plant Biology and Biotechnology, Almaty, 050040, Kazakhstan
I.A. Machmutova
Institute of Plant Biology and Biotechnology, Almaty, 050040, Kazakhstan
L.N. Karasholakova
Institute of Plant Biology and Biotechnology, Almaty, 050040, Kazakhstan
A.A. Christenko
Institute of Plant Biology and Biotechnology, Almaty, 050040, Kazakhstan
S.V. Kushnarenko
Institute of Plant Biology and Biotechnology, Almaty, 050040, Kazakhstan
Abstract
The creation of an in vitro collection of aseptic plants is the first stage for preservation of genetic diversity of barberry in a cryobank and production of mother plantations in natural habitats.Seedlings and plantlets of barberry were obtained from seeds and cuttings, respectively,using biotechnological methodsto produce aseptic plants. Scarification and stratification is required for seeds of some species that are difficult to germinate in wet perlite. Treatment of explants with 0.1% HgCl2 for 8 min is optimal for in vitro culture and produces 46.4%regeneration.In vitro plants were tested for the presence of infection in a specialized 523 medium to detectbacteria and fungi. On average,44.5% of the in vitroshoots in all the tested accessions were affected by endophytic infection. The composition of the nutrient medium was optimized as Murashige-Skoog medium with 166 mg/l CaCl2, 3.7 g/l MgSO4·7H2O, 30 g/l sucrose, 0.8 mg/l 6-benzylaminopurine, 0.02 mg/l indolebutyricacid, 0.1 mg/l gibberellic acid, 2 mg/l calcium pantothenate, 1 mg/l ascorbic acid, 1.75 g/l gelrite, 4 g/l agar, pH 5.7. Amaximum multiplication factorof 4.3 and high quality of regenerated plants were obtained using the optimized medium. A biotechnological regulation was developed to obtain an in vitrocollection of barberry plants anda collection consisting of 51 accessions was created. This collection will serve as the basis for the creation of cryobank of Kazakhstan barberry germplasm and for international exchange of plant resources.
Keywords
barberry, seeds, shoots, micropropagation, in vitro collection
Article Details
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