MASS SPECTROMETRY-BASED APPROACHES TO CHARACTERIZE MYCOBACTERIUM TUBERCULOSIS
Main Article Content
Authors
S. Atavliyeva
National Center for Biotechnology, 13/5, Korgalzhyn road, Astana, 010000, Kazakhstan
P. Tarlykov
National Center for Biotechnology, 13/5, Korgalzhyn road, Astana, 010000, Kazakhstan
E. Zholdybayeva
National Center for Biotechnology, 13/5, Korgalzhyn road, Astana, 010000, Kazakhstan
Ye. Ramankulov
National Center for Biotechnology, 13/5, Korgalzhyn road, Astana, 010000, Kazakhstan
Abstract
Mass spectrometry has greatly contributed to the study and understanding of the pathogenesis of human tuberculosis. Current methods of mass spectrometry have been rapidly evolving over the past two decades in response to the limitations of early proteomic studies. Modern proteomic research includes protein secretion, activation, degradation, and modifications, since post-translational modifications are an additional step in the evolution of pathogenic mycobacteria that gain virulence. The other application of mass spectrometry is in epidemiological studies of mycobacteria, in particular, determining the genetic spoligotype based on matrix-assisted laser desorption/ionization. This review explores M. tuberculosis studies carried out by mass spectrometry, including proteomic profiling with the shotgun-based approach and targeted proteomics. In addition, the most important post-translational modifications studied by mass spectrometry are described.
Keywords
mass spectrometry, Mycobacterium tuberculosis, proteome, spoligotyping
Article Details
References
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