Genetic Engineering Materials Bank of The National Center for Biotechnology
Main Article Content
Authors
A. Sarsen
National center for biotechnology, 13/5, Kоurgalzhyn road, Nur-Sultan, 010000, Kazakhstan
L.N.Gumilyov Eurasian National University, Satpayev str., 2, Nur-Sultan, 010000, Kazakhstan
M. Saginova
National center for biotechnology, 13/5, Kоurgalzhyn road, Nur-Sultan, 010000, Kazakhstan
L.N.Gumilyov Eurasian National University, Satpayev str., 2, Nur-Sultan, 010000, Kazakhstan
B. Mukanov
National center for biotechnology, 13/5, Kоurgalzhyn road, Nur-Sultan, 010000, Kazakhstan
L.N.Gumilyov Eurasian National University, Satpayev str., 2, Nur-Sultan, 010000, Kazakhstan
S. Aktayeva
National center for biotechnology, 13/5, Kоurgalzhyn road, Nur-Sultan, 010000, Kazakhstan
L.N.Gumilyov Eurasian National University, Satpayev str., 2, Nur-Sultan, 010000, Kazakhstan
Zh. Akishev
National center for biotechnology, 13/5, Kоurgalzhyn road, Nur-Sultan, 010000, Kazakhstan
A. Mussakhmetov
National center for biotechnology, 13/5, Kоurgalzhyn road, Nur-Sultan, 010000, Kazakhstan
A. Kiribayeva
National center for biotechnology, 13/5, Kоurgalzhyn road, Nur-Sultan, 010000, Kazakhstan
K. Baltin
National center for biotechnology, 13/5, Kоurgalzhyn road, Nur-Sultan, 010000, Kazakhstan
D. Silayev
National center for biotechnology, 13/5, Kоurgalzhyn road, Nur-Sultan, 010000, Kazakhstan
B. Khassenov
National center for biotechnology, 13/5, Kоurgalzhyn road, Nur-Sultan, 010000, Kazakhstan
Abstract
For the development of science-intensive technologies in biology, biotechnology and medicine, it is necessary to store valuable DNA samples: genes of enzymes, antigens, proteins for various purposes, and DNA loci in a depository. Storing DNA is inexpensive, and having such a depository will reduce the time spent on research in basic and applied science.
The genetically engineered materials bank has been formed at the National center for biotechnology. Now, the bank has 88 samples of cloned genes of enzymes, antigens, proteins and diagnostically significant loci. Plasmid vectors with cloned genes are stored at a temperature -20°С. Besides competent cells of Escherichia coli strain DH5α were transformed with vectors and recombinant strains were isolated. Recombinant strains carrying the required plasmid vectors are stored for cryopreservation at a temperature -80°С.
Keywords
biobank, DNA, strain, gene, protein
Article Details
References
www.addgene.org URL
Bhatt, K., et al., Molecular analysis of Bacillus velezensis KB 2216, purification and biochemical characterization of alpha-amylase. Int J BiolMacromol, 2020, 2020.10.1016/j.ijbiomac.2020.08.205.
Mussakhmetov, A., et al., A transient post-translational modification of active site cysteine alters binding properties of the parkinsonism protein DJ-1. BiochemBiophys Res Commun., 2018, vol.504, no.1, pp. 328-333. URL
Tsien, R.Y., The green fluorescent protein. Ann Rev Biochem., 1998, vol. 67, pp. 509-44.
Akishev Zh., Abeldenov S., Ramanculov E., Khassenov B. Expression of camel (Camelusbactrianus) and bovine (Bostaurus) chymosin in Pichia (Komagataella) pastoris. Journal of Biotechnology., 2017, vol.256S, pp.S73–S74. Crossref
Turgimbayeva, A. et al., Characterization of biochemical properties of an apurinic/apyrimidinic endonuclease from Helicobacter pylori. PLoS One, 2018, vol. 13, no. 8, pp. e0202232.