In Vitro and In Vivo Effects of Radio- Frequency Electromagnetic Fields
Main Article Content
Authors
D. A. Begimbetova
Laboratory of Translational Medicine and Life Sciences Technologies, National Laboratory Astana, Nazarbayev University 53, Kabanbay batyr ave., Nur-Sultan, 010000, Kazakhstan
D. M. Baiskhanova
Laboratory of Translational Medicine and Life Sciences Technologies, National Laboratory Astana, Nazarbayev University 53, Kabanbay batyr ave., Nur-Sultan, 010000, Kazakhstan
B. T. Matkarimov
Laboratory of Biosensors and Bioinstruments, National Laboratory Astana, Nazarbayev University 53, Kabanbay batyr ave., Nur-Sultan, 010000, Kazakhstan
Z. T. Shulgau
Laboratory of Toxicology and Pharmacology, National Center for Biotechnology, Korgalzhyn road, 13/5, Nur-Sultan, 010000, Kazakhstan
Abstract
In recent years, there has been a massive distribution of mobile phones and Wi-Fi networks. In this regard, concerns about their potential effects on living organisms, including humans, are increasing. Despite earlier assumptions about the absence of harmful effects of short-term exposure to radio frequency electromagnetic fields emitted by mobile phones and Wi-Fi networks, there is now increasing evidence of the potentially harmful effects of electromagnetic fields on the human body through the induction of oxidative stress and damage to DNA structure. It is assumed that prolonged exposure to electromagnetic fields can cause the development of various pathological conditions in the human body, including oncological diseases, impaired cognitive functions and sleep, etc.
This review analyzes and summarizes existing studies indicating various negative effects of radio frequency electromagnetic fields on human and animal cells. Since there is a large amount of conflicting data on the effect of electromagnetic fields on the human body, only further research can provide an answer to the possible negative effects of mobile phones and Wi-Fi networks usage.
Keywords
electromagnetic field, effects, in vitro, in vivo, radio frequency, apoptosis, DNA damage
Article Details
References
Andrzej Magiera, Jolanta Solecka. Mobile telephony and its effects on human health. Rocz Panstw Zakl Hig, 2019, vol. 70, no.3, pp. 225-234.
Ju Hwan Kim,1 Jin-Koo Lee,1 Hyung-Gun Kim, et.al. Possible Effects of Radiofrequency Electromagnetic Field Exposure on Central Nerve System. Biomol Ther (Seoul), 2019, vol. 27, no.3, pp. 265–275.
Diem E., Schwarz C., Adlkofer F. et.al. Non-thermal DANN breakage by mobile-phone radiation (1800 MHz) in human fibroblasts and in transformed GFSH-R17 rat granulosa cells in vitro. Mutat. Res., 2005, vol. 583, pp. 178–183.
Joubert V., Bourthoumieu S., Leveque P. et.al. Apoptosis is induced by radiofrequency fields through the caspase-independent mitochondrial pathway in cortical neurons. Radiat. Res., 2008, vol. 169, pp. 38–45.
Nakamura H., Matsuzaki I., Hatta K. et.al. Non-thermal effects of mobile-phone frequency microwaves on uteroplacental functions in pregnant rats. Reprod. Toxicol., 2003, vol. 17, pp. 321–326.
Joubert V., Bourthoumieu S., Leveque Ph., et.al. Apoptosis is induced by Radiofrequency Fields through the Caspase-Independent Mitochondrial Pathway in Cortical neurons. Radiation Research, 2008, vol. 169, no.1, pp. 38-45.
Cregan S. P., Fortin A., MacLaurin J. G. et.al. Apoptosis-inducing factor is involved in the regulation of caspaseindependent neuronal cell death. J. Cell Biol., 2002, vol. 158, pp. 507–517.
Liou A.K., Zhou Z., Pei W. et.al. BimEL up-regulation potentiates AIF translocation and cell death in response to MPTP. FASEB J., 2005, vol. 19, pp. 1350–1352.
Stoica B.A., Movsesyan V.A., Knoblach S.M. et.al. Ceramide induces neuronal apoptosis through mitogen-activated protein kinases and causes release of multiple mitochondrial proteins. Mol. Cell Neurosci., 2005, vol 29, pp. 355–371.
Hans G., Malgrange B., Lallemend F., et.al. Beta-carbolines induce apoptosis in cultured cerebellar granule neurons via mitochondrial pathway. Neuropharmacology, 2005, vol. 48, pp. 105–117.
Cheung E.C., Melanson-Drapeau L., Cregan S.P., et.al. Apoptosis-inducing factor is a key factor in neuronal cell death propagated by BAX-dependent and BAX-independent mechanisms. J. Neurosci., 2005, vol. 25, pp. 1324–1334.
Wang H., Yu S.W., Koh D.W., et.al. Apoptosisinducing factor substitutes for caspase executioners in NMDA-triggered excitotoxic neuronal death. J. Neurosci., 2004, vol. 24, pp. 10963–10973.
Plesnila N., Zhu C., Culmsee C., et.al. Nuclear translocation of apoptosis-inducing factor after focal cerebral ischemia. J. Cereb. Blood Flow Metab., 2004, vol. 24, pp. 458–466.
Campisi A., Caccamo D., Raciti G. et.al. Glutamate-induced increases in transglutaminase activity in primary cultures of astroglial cells, Brain Res., 2003, vol. 978, pp. 24–30.
Zmyslony M., Politanski P., Rajkowska E., et.al. Acute exposure to 930MHz CWelectromagnetic radiation in vitro affects reactive oxygen species level in rat lymphocytes treated by iron ions. Bioelectromagnetics, 2004, vol. 25, pp. 324–328.
Yao K., Wu W., Wang K., et.al. Electromagnetic noise inhibits radiofrequency radiation-induced DNA damage and reactive oxygen species increase in human lens epithelial cells. Mol. Vis., 2008, vol. 14, pp. 964–969.
Tice R.R., Hook G.G., Donner M., et.al. Genotoxicity of radiofrequency signals. I. Investigation of DNA damage and micronuclei induction in cultured human blood cells. Bioelectromagnetics, 2002, vol. 23, pp. 113–126.
Lixia S., Yao K., Kaijun W. et.al. Effects of 1.8GHz radiofrequency field on DNA damage and expression of heat shock protein 70 in human lens epithelial cells. Mutat. Res., 2006, vol. 602, pp. 135–142.
Mashevich M., Folkman D., Kesar A. et.al. Exposure of human peripheral blood lymphocytes to electromagnetic fields associated with cellular phones leads to chromosomal instability. Bioelectromagnetics, 2003, vol. 24, pp. 82–90.
Duan W., Liu C., Zhang L., et.al. Comparison of the genotoxic effects induced by 50 hz extremely low-frequency electromagnetic fields and 1800 mhz radiofrequency electromagnetic fields in gc-2 cells. Radiat. Res., 2015, vol. 183, pp. 305–314.
Hou Q., Wang M., Wu S., et.al. Oxidative changes and apoptosis induced by 1800-MHz electromagnetic radiation in NIH/3t3 cells. Electromagn. Biol. Med., 2015, vol. 34, pp. 85–92.
Sun C., Wei X., Fei Y., et.al. Mobile phone signal exposure triggers a hormesis-like effect in Atm+/+ and Atm-/- mouse embryonic fibroblasts. Sci. Rep., 2016, vol. 6, pp. 37423.
Bourthoumieu S., Joubert V., Marin B. et.al. Cytogenetic studies in human cells exposed in vitro to GSM-900 MHz radiofrequency radiation using R-banded karyotyping. Radiat. Res. 2010, vol. 174, pp. 712–718.
Akhavan-Sigari R., Mazloum F.B.M., Ariabod V., et.al. Connection between cell phone use, p53 gene expression in different zones of glioblastoma multiforme and survival prognoses. Rare Tumors, 2016, vol. 6, pp. 5350.
Lu Y.S., Huang B.T., Huang, Y.X. Reactive oxygen species formation and apoptosis in human peripheral blood mononuclear cell induced by 900 MHz mobile phone radiation. Oxid. Med. Cell. Longev. 2012, vol. 2012, pp. 740280.
Trivino Pardo J.C., Grimaldi S., Taranta M., Naldi I. et.al. Microwave electromagnetic field regulates gene expression in T-lymphoblastoid leukemia CCRF-CEM cell line exposed to 900 MHz. Electromagn. Biol. Med., 2012, vol. 31, pp. 1–18.
Roux D., Girard S., Paladian F., et.al. Human keratinocytes in culture exhibit no response when exposed to short duration, low amplitude, high frequency (900 MHz) electromagnetic fields in a reverberation chamber. Bioelectromagnetics, 2011, vol. 32, pp. 302–311.
Tkalec M., Stambuk A., Srut M. et.al. Oxidative and genotoxic effects of 900 MHz electromagnetic fields in the earthworm Eisenia fetida. Ecotoxicol. Environ. Saf., 2013, vol. 90, pp. 7–12.
Consales C., Merla C., Marino C. et.al. Electromagnetic fields, oxidative stress, and neurodegeneration. Int. J. Cell Biol., 2012, vol. 2012, pp. 683897.
Ntzouni M.P., Stamatakis A., Stylianopoulou F., et.al. Short-term memory in mice is affected by mobile phone radiation. Pathophysiology, 2011, vol. 18, pp. 193–199.
Maskey D., Pradhan J., Aryal B. et.al. Chronic 835-MHz radiofrequency exposure to mice hippocampus alters the distribution of calbindin and GFAP immunoreactivity. Brain Res., 2010, vol. 1346, pp. 237–246.
Ammari, M.; Lecomte, A.; Sakly, M.; et.al. Exposure to GSM 900 MHz electromagnetic fields affects cerebral cytochrome c oxidase activity. Toxicology, 2008, vol. 250, pp. 70–74.
Jiang D.P., Li J., Zhang, J. et.al. Electromagnetic pulse exposure induces overexpression of beta amyloid protein in rats. Arch. Med. Res., 2013, vol. 44, pp. 178–184.
Dragicevic N., Bradshaw P.C., Mamcarz M. et.al. Long-term electromagnetic field treatment enhances brain mitochondrial function of both Alzheimer’s transgenic mice and normal mice: A mechanism for electromagnetic field-induced cognitive benefit? Neuroscience, 2011, vol. 185, pp. 135–149.
Banaceur S., Banasr S., Sakly M., et.al. Whole body exposure to 2.4 GHz WIFI signals: Effects on cognitive impairment in adult triple transgenic mouse models of Alzheimer’s disease (3× Tg-AD). Behav. Brain Res., 2013, vol. 240, pp. 197–201.
Filali M., Lalonde R., Theriault P. et.al. Cognitive and non-cognitive behaviors in the triple transgenic mouse model of Alzheimer’s disease expressing mutated APP, PS1, and Mapt (3× Tg-AD). Behav. Brain Res., 2012, vol. 234, pp. 334–342.
Carpenter D.O. Human disease resulting from exposure to electromagnetic fields1). Rev. Environ. Health, 2013, vol. 28, pp. 159–172.
Terro F., Magnaudeix A., Crochetet, M., et.al. GSM-900MHz at low dose temperature-dependently downregulates alpha-synuclein in cultured cerebral cells independently of chaperone-mediated-autophagy. Toxicology, 2012, vol. 292, pp. 136–144.
Fragopoulou A.F., Samara A., Antonelou M.H., et al. Brain proteome response following whole body exposure of mice to mobile phone or wireless DECT base radiation. Electromagn. Biol. Med., 2012, vol. 31, pp. 250–274.
Li H.J., Guo L.M., Yang L.L., et.al. Electromagnetic-pulse-induced activation of p38 MAPK pathway and disruption of blood-retinal barrier. Toxicol. Lett., 2013, vol. 220, pp. 35–43.
Zhou J.X., Ding G.R., Zhang J., et.al. Detrimental effect of electromagnetic pulse exposure on permeability of in vitro blood-brain-barrier model. Biomed. Environ. Sci., 2013, vol. 26, pp. 128–137.
Nittby H., Brun A., Eberhardt J., et.al. Increased blood-brain barrier permeability in mammalian brain 7 days after exposure to the radiation from a GSM-900 mobile phone. Pathophysiology, 2009, vol. 16, pp. 103–112.
Pelletier A., Delanaud S., Decima P., et.al. Effects of chronic exposure to radiofrequency electromagnetic fields on energy balance in developing rats. Environ. Sci. Pollut. Res. Int., 2013, vol. 20, pp. 2735–2746.
Aydin B., Akar A. Effects of a 900-MHz electromagnetic field on oxidative stress parameters in rat lymphoid organs, polymorphonuclear leukocytes and plasma. Arch. Med. Res., 2011, vol. 42, pp. 261–267.
Sekeroglu V., Akar A., Sekeroglu Z.A. Cytotoxic and genotoxic effects of high-frequency electromagnetic fields (GSM 1800 MHz) on immature and mature rats. Ecotoxicol. Environ. Saf., 2012, vol. 80, pp. 140–144.