OPTIMIZATION OF THE MAIN PARAMETERS OF A LATERAL FLOW ASSAY BASED ON THE RECOMBINANT Р32 ANTIGEN OF LUMPY SKIN DISEASE VIRUS
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Authors
K. Tursunov
National Center for Biotechnology, 13/5 Korgalzhynskoye Road, Astana, 010000
L. Tokhtarova
National Center for Biotechnology, 13/5 Korgalzhynskoye Road, Astana, 010000
D. Kanayev
National Center for Biotechnology, 13/5 Korgalzhynskoye Road, Astana, 010000
R. Mustafina
S.Seifullin Kazakh Agrotechnical Research University, 62 Zhenis Avenue, Astana, 010001
K. Mukantayev
National Center for Biotechnology, 13/5 Korgalzhynskoye Road, Astana, 010000
Abstract
Lumpy skin disease is a highly contagious infectious transboundary disease of cattle, resulting in significant economic losses. The wide and rapid spread of lumpy skin disease to new territories emphasizes the objective need to improve diagnostic methods. The article outlines the findings of lateral flow assay parameter optimization for lumpy skin disease detection using recombinant P32 antigen. It was found that the optimal amount of protein for immobilization on 20 nm colloidal gold particles was 20 µg/mL. Bovine serum albumin at a final concentration of 0.25% was used to stabilize the conjugate. The optimal dilution of the conjugate was 1:2, and the concentration of recombinant protein and anti-species antibodies for application to the membrane was 500 μg/ml and 250 μg/ml, respectively. The colloidal gold conjugate interacted with antibodies and specifically reacted with the recombinant protein. No cross-reaction with other proteins was noticed at the same time. The created express test allowed for the detection of antibodies from immunized animals when the serum was diluted up to 1:200, however, 1:50 was the ideal ratio. The conjugate remained active after 90 days of storage at room temperature. The findings demonstrate the express method's potential for use in serological research for the prevention and control of the spread of lumpy skin disease.
Keywords
lumpy skin disease virus, diagnostics, colloidal gold, P32 antigen, lateral flow assay, vaccination
Article Details
References
Namazi, F., Tafti, A.K. Lumpy skin disease, an emerging transboundary viral disease: A review // Vet. Med. Sci. – 2021. – Vol. 7, № 3. – P. 888–896. Crossref.
Tuppurainen, E.S.M., Oura, C.A.L. Review: Lumpy Skin Disease: An Emerging Threat to Europe, the Middle East and Asia // Transboundary and Emerging Diseases. – 2012. – Vol. 59. – P. 40–48. Crossref.
Ratyotha, K., Prakobwong, S., and Piratae, S. Lumpy skin disease: A newly emerging disease in Southeast Asia // Veterinary World. – 2022. – Vol. 15, № 12. – P. 2764–2771. Crossref.
Anwar, A., Na-Lampang, K., Preyavichyapugdee, N., Punyapornwithaya, V. Lumpy Skin Disease Outbreaks in Africa, Europe, and Asia (2005-2022): Multiple Change Point Analysis and Time Series Forecast // Viruses. – 2022. – Vol. 14, № 10. 2203. Crossref.
Ospanov, E.K., Kaimoldina, S.E., Kirpichenko, V.V., Kenesbek, M.F. Epizootological characteristics of the territory of the Republic of Kazakhstan on bovine lumpy skin disease in for 2016-2021 // Izdenister Natigeler. – 2022. – Vol. 1, № 93. – P. 5–14. Crossref.
Berguido, F.J., Gelaye, E., Liu, Y., Davaasuren, B., Krstevski, K., Djadjovski, I., Ivanova, E., Goujgoulova, G., Loitsch, A., Tuppurainen, E., et al. Development and Optimization of Indirect ELISAs for the Detection of Anti-Capripox virus Antibodies in Cattle, Sheep, and Goat Sera // Microorganisms. – 2022. – Vol. 10, № 93. 1956. Crossref.
Tuppurainen, E., Dietze, K., Wolff, J., Bergmann, H., Beltran-Alcrudo, D., Fahrion, A., Lamien, C.E., Busch, F., Sauter-Louis, C., Conraths, F.J., et al. Review: Vaccines and Vaccination against Lumpy Skin Disease // Vaccines. – 2021. – Vol. 9. 1136. Crossref.
Tuppurainen, E.S.M., Venter, E.H., Shisler, J.L., Gari, G., Mekonnen, G.A., Juleff, N., Lyons, N.A., De Clercq, K., Upton C., Bowden, T.R., Babiuk, S., Babiuk L.A. Review: Capripoxvirus Diseases: Current Status and Opportunities for Control // Transboundary and Emerging Diseases. – 2017. – Vol. 64. – P. 729–745. Crossref.
OIE. Lumpy skin disease. In Manual of Diagnostic Tests and Vaccines for Terrestrial Animals; World Organisation for Animal Health: Paris, France. – 2021. – Vol. 3.
Sultankulova, K.T., Shynybekova, G.O., Issabek, A.U., Mukhami, N.N., Melisbek, A.M., Chervyakova, O.V., Kozhabergenov, N.S., Barmak, S.M., Bopi, A.K., Omarova, Z.D., et al. The Prevalence of Pathogens among Ticks Collected from Livestock in Kazakhstan // Pathogens. – 2022. – Vol. 11. 1206. Crossref.
Frens, G. Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions // Nature Phys. Sci. – 1973. – Vol. 241. – P. 20–22. Crossref.
Bowden, T.R., Coupar, B.E., Babiuk, S.L., White, J.R., Boyd, V., Duch, C J., Shiell, B.J., Ueda, N., Parkyn, G.R., Copps, J.S., Boyle, D.B. Detection of antibodies specific for sheeppox and goatpox viruses using recombinant capripoxvirus antigens in an indirect enzyme-linked immunosorbent assay // J. Virol. Methods. – 2009. – Vol. 161, № 1. – P. 19–29. Crossref.
Sthitmatee, N., Tankaew, P., Modethed, W., Rittipornlertrak, A., Muenthaisong, A., et al. Development of in-house ELISA for detection of antibodies against lumpy skin disease virus in cattle and assessment of its performance using a bayesian approach // Heliyon. – 2023. – Vol. 9. e13499. Crossref.
Tian, H., Chen, Y., Wu, J., Shang, Y., Liu, X. Serodiagnosis of sheeppox and goatpox using an indirect ELISA based on synthetic peptide targeting for the major antigen P32 // Virology Journal. – 2010. – Vol. 7, 245. Crossref.
Babiuk, S., Wallace, D.B., Smith, S.J., Bowden, T.R., Dalman, B., Parkyn, G., Copps, J., Boyle, D.B. Detection of Antibodies Against Capripoxviruses Using an Inactivated Sheeppox Virus ELISA // Transboundary and Emerging Diseases. – 2009. – Vol. 56. – P. 132–141. Crossref.
Xia, S., Yu, Z., Liu, D., Xu, C., Lai, W. Developing a novel immunochromatographic test strip with gold magnetic bifunctional nanobeads (GMBN) for efficient detection of Salmonella choleraesuis in milk // Food Control. – 2016. – Vol. 59. – P. 507–512. Crossref.
Luo, J., Sun, H., Zhao, X., Wang, S., Zhuo, X., Yang, Y., Chen, X., Yao, C., Du, A. Development of an immunochromatographic test based on monoclonal antibodies against surface antigen 3 (TgSAG3) for rapid detection of Toxoplasma gondii // Veterinary Parasitology. – 2018. –Vol. 252. – P. 52–57. Crossref.
Yu, J., Lin, Y., Cao, Y., Li, X., Liao, D., Ye, Y., Pan, M., Ye, J., Wei, Y., Xiao, L., Tang, J., Kang, R., Xie, J., Zhou, L. Development and application of a colloidal gold test strip for the rapid detection of the infectious laryngotracheitis virus // Poultry Science. – 2020. – Vol. 99, № 5. – P. 2407–2415. Crossref.
Senthilkumar, K., Ravikumar, G. Lateral flow assay for rapid serodiagnosis of bovine leptospirosis // IJVR. – 2022. – Vol. 23, № 78. – P. 7–11. Crossref.
Abdalhamed A.M, Naser S.M, Mohamed A.H, Zeedan G.S.G. Development of gold nanoparticles-lateral flow test as a novel field diagnostic assay for detecting foot-and-mouth disease and lumpy skin disease viruses // Iranian Journal of Microbiology. – 2022. – Vol. 14, № 4. – P. 574-586. Crossref.
Cavalera, S., Pezzoni, G., Grazioli, S., Brocchi, E., Baselli, S., Lelli, D., Colitti, B., Serra, T., Nardo, F.D., Chiarello, M. et al. Investigation of the “Antigen Hook Effect” in Lateral Flow Sandwich Immunoassay: The Case of Lumpy Skin Disease Virus Detection // Biosensors. – 2022. – Vol. 12, 739. Crossref.
Urusov, A.E., Zherdev, A.V. Study of dependences of analytical parameters of immunochromatographic test systems from the regime of immobilization of immunoreagents on membranes // Modern problems of science and education. – 2013. – № 3 – P. 445–445.
Sukumaran, A., Thomas, T., Thomas, R., Thomas, R.E., Paul, J.K., Vasudevan, D.M. Development and Troubleshooting in Lateral Flow Immunochromatography Assays // Ind J Clin Biochem. – 2021. – Vol. 36, № 2. – P. 208–212. Crossref.