OPTIMIZATION OF PROCESS PARAMETERS FOR CULTIVATION OF BACTERIOPHAGES PROMISING FOR BIOLOGICAL CONTROL OF PHYTOPATHOGENIC BACTERIA OF GENUS PSEUDOMONAS
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Authors
T.A. Pilipchuk
Institute of Microbiology, National Academy of Sciences of Belarus, 2, Kuprevich str., Minsk, 220141, Republic of Belarus
A.D. Gerasimovich
Institute of Microbiology, National Academy of Sciences of Belarus, 2, Kuprevich str., Minsk, 220141, Republic of Belarus
I.N. Ananyeva
Institute of Microbiology, National Academy of Sciences of Belarus, 2, Kuprevich str., Minsk, 220141, Republic of Belarus
E.I. Kolomiets
Institute of Microbiology, National Academy of Sciences of Belarus, 2, Kuprevich str., Minsk, 220141, Republic of Belarus
Abstract
Cultural parameters of bacteriophages screened for lytic activity toward host bacterial strain P. helmanticensis BIM В-582 D were investigated. The following conditions were shown to be optimal for cultivation of indicator culture and it’s lysis by Pseudomonas phages BIM ВV-45 D, BIM ВV-46 D, BIM ВV-47 D, BIM ВV-50 D, BIM ВV-53 D, BIM ВV-61 D in laboratory fermenters ANKUM-3M: nutrient medium – fish meal hydrolysate broth, temperature – 28°C, stirrer agitation rate – 160 rpm, aeration rate – 1 l air/ l medium per min. Biopreparation produced under optimized conditions and composed of six afore-mentioned phages display high lytic activity both in regard to the host strain and to phytopathogenic bacteria Pseudomonas syringae.
Keywords
bacteriophages, cultural parameters, nutrient medium, temperature
Article Details
References
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