SPECIFICITY OF LYMPHOCYTES BINDING THE CAPSULE ANTIGEN OF PLAGUE BACTERIA AND AN IMMUNOLOGICAL REAGENT FOR THEIR DETECTION
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Authors
B.V. Karalnik
H. Zhumatov Scientific Hygiene and Epidemiology Center, 34,Makataev str., Almaty, 050002, Kazakhstan
G.B. Zhunussova
H. Zhumatov Scientific Hygiene and Epidemiology Center, 34,Makataev str., Almaty, 050002, Kazakhstan
T.G. Denisova
H. Zhumatov Scientific Hygiene and Epidemiology Center, 34,Makataev str., Almaty, 050002, Kazakhstan
T.S. Ponomareva
M. Aykimbayev Kazakh Scientific Center of Quarantine and Zoonotic Diseases, 14, Kapalskaya str., Almaty, 050054, Kazakhstan
P.N. Deryabin
M. Aykimbayev Kazakh Scientific Center of Quarantine and Zoonotic Diseases, 14, Kapalskaya str., Almaty, 050054, Kazakhstan
B.B. Atshabar
M. Aykimbayev Kazakh Scientific Center of Quarantine and Zoonotic Diseases, 14, Kapalskaya str., Almaty, 050054, Kazakhstan
A.A. Turuzhanova
H.Zhumatov Scientific Hygiene and Epidemiology Center, 34,Makataev str., Almaty, 050002, Kazakhstan
T.I. Tugambayev
M. Aykimbayev Kazakh Scientific Center of Quarantine and Zoonotic Diseases, 14, Kapalskaya str., Almaty, 050054, Kazakhstan
S.B. Zakaryan
M. Aykimbayev Kazakh Scientific Center of Quarantine and Zoonotic Diseases, 14, Kapalskaya str., Almaty, 050054, Kazakhstan
Abstract
The aim of this study was to analyse the specificity of an immunological reagent developed to identify lymphocytes with receptors for the dominant antigen (F1) of plague bacteria (lymphocytes with F1 receptors; LfR). Rabbits were used to test immunogens, consisting of live and inactivated plague vaccine, and inhibitors of LfR. Interactions between immunological reagents, consisting of suspensions of three inactivated Yersinia species (Yersinia pestis, Yersinia enterocolitica and Yersinia pseudotuberculosis); antigens (F1 and lipopolysaccharide of Y. pestis); and the immunomodulators, betaleukin (recombinant interleukin 1β) and polyoxidonium were also investigated. Intercell adhesion methods were used to detect LfR and determine their inhibition. The specificity of the reagent developed was experimentally determined using homologic and heterologic LfR antigens. The clonal nature of LfR and the development of weakly expressed, non-specific factors during the early phase of the adaptive immune response were confirmed after immunisation of the plague vaccination model, by inhibition of LfR identification using various antigens. The expression of a weak, non-specific response did not affect the specific detection of LfR, indicating the specificity of the developed immunological reagent as a clone of LfR.
Keywords
plague, early phase of adaptive response, immunological reagent, specificity
Article Details
References
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