THE ANTIGENICITY OF THE OUTER MEMBRANE PROTEINS OF BRUCELLA

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Authors

A.K. Bulashev

S. Seifullin Kazakh Agro-Technical University, 2 Altynsarin Str., Astana, 010011, Kazakhstan

Zh.A. Suranshiev

S. Seifullin Kazakh Agro-Technical University, 2 Altynsarin Str., Astana, 010011, Kazakhstan

A.Kh. Zhumalin

S. Seifullin Kazakh Agro-Technical University, 2 Altynsarin Str., Astana, 010011, Kazakhstan

K.А. Tursunov

S. Seifullin Kazakh Agro-Technical University, 2 Altynsarin Str., Astana, 010011, Kazakhstan

Abstract

The effectiveness of brucellosis eradication measures depends on the timely diagnosis and isolation of infected animals. Commercial ELISA-kits for diagnosis of brucellosis use lipopolysaccharides (LPS) as target antigens. It is well known that use of these cell wall components can result in false-positive results due to cross-reaction of antibodies against Brucella spp. with other gram-negative bacteria. Outer membrane proteins (OMP) are of great interest, as they are specific, not only to the Brucella genus, but also for particular species. OMP extracted from Brucella abortus 19 and Brucella melitensis Rev-1 were more antigenic than LPS by agglutination and complement fixation tests using serum samples from cows positive for brucellosis. B. abortus OMP also had significantly greater diagnostic value than an antigenic recombinant protein with a molecular weight of 26 kDa (rBP26). A noticeable advantage of OMP over rBP26 was also established by serological investigation of cows vaccinated with the rough strain, B. abortus RB-51. The results suggest that in the development of ELISA-kits for diagnosis of bovine brucellosis not a single protein, but several recombinant proteins, with distinct antigenic properties should be used. Among OMP protein fractions from both Brucella species, those with the greatest potential as diagnostic antigens had molecular weights of 19 and 15 kDa, in addition to a B. melitensis protein, with a molecular weight of 12 kDa. These proteins demonstrated antigenicity in western blot analysis using serum antibodies from cows positive for brucellosis by serological tests and PCR.

Keywords

brucellosis, Brucella abortus, Brucella melitensis, outer membrane proteins, antigenicity, ELISA

Article Details

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