TY - JOUR AU - Ismagulova, G.A. AU - Baizhumanova, S.S. AU - Iskakova, G.A. AU - Baisaparova, D.O. AU - Mendesh, A.M. AU - Tussipova, A.A. AU - Skiba, Yu.A. AU - Maltseva, E.R. AU - Naizabayeva, D.A. AU - Esimbekova, M.A. AU - Mukin, K.B. PY - 2022/10/28 Y2 - 2024/03/29 TI - SCREENING OF GENES RESPONSIBLE FOR β-GLUCAN SYNTHESIS IN GRAIN VARIETIES JF - Eurasian Journal of Applied Biotechnology JA - Eurasian Journal of Applied Biotechnology VL - IS - 4 SE - Articles DO - 10.11134/btp.4.2022.10 UR - https://biotechlink.org/index.php/journal/article/view/406 SP - 75-82 AB - <p>Currently, β-glucan has a great importance for human life. Because it reduces the risks of many chronic diseases such as diabetes, cancer and digestive disorders. Due to these properties, β-glucan is a natural substance that can be used in pharmaceutical preparations for the prevention and treatment of many chronic diseases. Therefore, an analysis was made for crops rich in sources of β-glucans - 100 varieties of wheat, 50 varieties of barley and 50 varieties of oats, and a collection of genomic DNA was made. Each variety was screened to identify the genes responsible for the synthesis of β-glucans. In the frames of this study, 10 genes were considered.  As a result, these genes have been identified in barley, wheat, and oat varieties.</p><p> For the isolation of β-glucan, the methods of acid extraction, microwave, and heating at high temperature were used. A modified method for isolating β-glucan from oatmeal was developed. According to the results, the acid extraction method proved to be more effective. It is easy to obtain pure β-glucan by this method, and the use of citric acid is safer and more affordable than conventional acids. The improved acid extraction method showed that the concentration of pure β-glucan per 10 g of wheat grain was 66.6%, and the total yield of β-glucan extract increased to 11.3%.</p> ER -