TY - JOUR AU - Pilipchuk, T.A. AU - Gerasimovich , A.D. AU - Ananyeva, I.N. AU - Kolomiets, E.I. PY - 2021/09/24 Y2 - 2024/03/28 TI - OPTIMIZATION OF PROCESS PARAMETERS FOR CULTIVATION OF BACTERIOPHAGES PROMISING FOR BIOLOGICAL CONTROL OF PHYTOPATHOGENIC BACTERIA OF GENUS PSEUDOMONAS JF - Eurasian Journal of Applied Biotechnology JA - Eurasian Journal of Applied Biotechnology VL - IS - 3 SE - Articles DO - 10.11134/btp.3.2021.3 UR - https://biotechlink.org/index.php/journal/article/view/39 SP - AB - <p>Cultural parameters of bacteriophages screened for lytic activity toward host bacterial strain <em>P.&nbsp;helmanticensis </em>BIM В-582 D were investigated. The following conditions were shown to be optimal for cultivation of indicator culture and it’s lysis by <em>Pseudomonas phages </em>BIM ВV-45 D, BIM ВV-46 D, BIM ВV-47 D, BIM ВV-50 D, BIM ВV-53 D, BIM ВV-61 D in laboratory fermenters ANKUM-3M: nutrient medium – fish meal hydrolysate broth, temperature – 28°C, stirrer agitation rate – 160 rpm, aeration rate – 1 l air/ l medium per min. Biopreparation produced under optimized conditions and composed of six afore-mentioned phages display high lytic activity both in regard to the host strain and to phytopathogenic bacteria <em>Pseudomonas syringae</em>.</p> ER -