TY - JOUR AU - Smagulova, A.M. AU - Uakhit , R.S. AU - Lider , L.А. AU - Lyalchenko , А.S. AU - Kiyan , V.S. PY - 2022/07/09 Y2 - 2024/03/29 TI - Distribution and genetic features of the causative agent of Alaria alata in wild carnivores of the steppe and forest-steppe zones of Kazakhstan JF - Eurasian Journal of Applied Biotechnology JA - Eurasian Journal of Applied Biotechnology VL - IS - 2 SE - Articles DO - 10.11134/btp.2.2022.6 UR - https://biotechlink.org/index.php/journal/article/view/345 SP - 38-43 AB - <p>This article presents data on the study of the <em>Alaria spp.</em> pathogen isolated from wild carnivores (corsacs, foxes, wolves) caught in the territory of Karaganda, Kostanay, Akmola, and East Kazakhstan regions in the period from 2019 to 2022. As a result of the autopsy of wild animals, a collection of <em>Alaria spp.</em> was collected, the intensity of invasion varied from 1 to 1489 samples/animal, and the prevalence of invasion ranged from 10 to 36.11%. The primary taxonomic affiliation of the pathogen was carried out using determinants. Genetic identification was carried out by amplification of a section of the <em>internal transcribed spacer 2</em> (<em>ITS2</em>) marker gene. The resulting 557 bp amplicons. sequenced using the Sanger method. The nucleotide sequences were deposited in the international GenBank database under accession numbers: from the fox - OM630451, ON248128, ON248130, from the corsac - OM630460, ON248043, ON248044, ON248045, from the wolf - ON358106. Bioinformatics analysis to build a phylogeny showed that the samples of <em>Alaria alata</em> studied by us were combined into a separate cluster by identity, which ranged from 90-98%.</p> ER -