THE EFFECT OF MUTAGENS ON CALLUS REGENERATION

Main Article Content

Authors

N. Iksat

L.N. Gumilyov Eurasian national university, Nur-Sultan, 010000, Kazakhstan

Zh. Masalimov

L.N. Gumilyov Eurasian national university, Nur-Sultan, 010000, Kazakhstan

R. Omarov

L.N. Gumilyov Eurasian national university, Nur-Sultan, 010000, Kazakhstan

Abstract

in this work were studied the characteristics of the development of F. tataricum and F. esculentum callus cultures in vitro when they were treated with chemical mutagen solutions - MH, MNU (0.05; 0.1; 0.2; 0.5; 1; 2 mM). When compared to the control on RX medium, the calli treated with the MNU mutagen died more often at higher concentrations (0.5; 1; 2 mM), indicating the effectiveness of the mutagenic effect. The survival rate of callus cultures subcultivated on a medium containing lower concentrations of mutagens (0.05; 0.1; 0.2 mM) was quite high when compared to higher concentrations, but low when compared to the control. The role of mutagenic factors in increasing genotypic variability, as well as the method of obtaining a new source material for various aspects of the buckwheat breeding process, have not been studied.

Keywords

mutagens, Fagopyrum, genotypic variability, callus, DNA fragmentation

Article Details

References

Kamalova, G. V., Akulov, A. N., Rumyantseva, N. I. Comparison of redox state of cells of tatar buckwheat morphogenic calluses and non-morphogenic calluses obtained from them //Biochemistry (Moscow). – 2009. – V. 74. – No. 6. – P. 686-694.

Lee, S. Y. et al. An efficient protocol for shoot organogenesis and plant regeneration of buckwheat (Fagopyrum esculentum Moench.) //Romanian Biotechnological Letters. – 2009. – V. 14. – No. 4. – P. 4524-4529.

Luthar, Z., Fabjan, P., Mlinarič, K. Biotechnological methods for buckwheat breeding //Plants. – 2021. – V. 10. – No. 8. – P. 1547.

Gupta, N. et al. Expression of flavonoid biosynthesis genes vis-à-vis rutin content variation in different growth stages of Fagopyrum species //Journal of plant physiology. – 2011. – V. 168. – No. 17. – P. 2117-2123.

Penin, A. A. et al. High-resolution transcriptome atlas and improved genome assembly of common buckwheat, Fagopyrum esculentum //Frontiers in plant science. – 2021. – V. 12. – P. 612382.

Rumyantseva, N. I. et al. Organogenesis and somatic embryogenesis in culture of two buckwheat species //Soviet plant physiology. – 1989. – V. 36. – No. 1. – P. 152-158.

Bidabadi, S. S., Jain, S. M. Cellular, molecular, and physiological aspects of in vitro plant regeneration //Plants. – 2020. – V. 9. – No. 6. – P. 702.

De Souza, L. P., Borghi, M., Fernie, A. Plant single-cell metabolomics—challenges and perspectives //International Journal of Molecular Sciences. – 2020. – V. 21. – No. 23. – P. 8987.

Khan, A. et al. Biomarker-based evaluation of cytogenotoxic potential of glyphosate in Vigna mungo (L.) Hepper genotypes //Environmental Monitoring and Assessment. – 2021. – V. 193. – No. 2. – P. 1-13.

Choyal, P. et al. Chemical manipulation of source and sink dynamics improves significantly the root biomass and the withanolides yield in Withania somnifera //Industrial Crops and Products. – 2022. – V. 188. – P. 115577.

Hassanzadeh, F., Zakaria, R. A., Azad, N. H. Polyploidy induction in Salvia officinalis L. and its effects on some morphological and physiological characteristics //Cytologia. – 2020. – V. 85. – No. 2. – P. 157-162.