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K.J. Zhambakin

Institute of Plant Biology and Biotechnology, 45, Timirjazeva str., 050040, Almaty, Kazakhstan

M.H. Shamekova

Institute of Plant Biology and Biotechnology, 45, Timirjazeva str., 050040, Almaty, Kazakhstan

D.V. Volkov

Institute of Plant Biology and Biotechnology, 45, Timirjazeva str., 050040, Almaty, Kazakhstan

A.K. Zatybekov

Institute of Plant Biology and Biotechnology, 45, Timirjazeva str., 050040, Almaty, Kazakhstan


Commercial cultivation of safflower appeal lies in its high resistance to drought and high quality of the oil obtained. At the same time, despite the fact that safflower has been known since antiquity, this culture is still poorly understood. Safflower highly varies in morphology, content and composition of the oil, depending on the region of cultivation. The review provides data on the use of safflower as a plant for multipurpose use in food industry, medicine and as a food source for animals and birds. Demand for safflower oil has been increasing over the last period due to the high level content of polyunsaturated fatty acids. For industrial applications, the role of breeding aimed at obtaining varieties with certain combination of oil, safflower oleic, linoleic fatty acids. To improve selection, applications of molecular markers in plant breeding and safflower seeds using culture cells and tissues and methods of genetic engineering are found to be promising. Molecular markers for safflower are used to study the phylogenetic relationships and the origin of the Carthamus tinctorius species, to control the commercial use of varieties - the food or the technical direction, and to identify genes associated with specific selection and valuable traits. Use of male gametophyte culture for production of homozygous lines is also promising. Application of genetic engineering techniques may result into development of new safflower varieties with high level of oleic acid and resistant to non-selective herbicide. The problems and prospects of safflower cultivation in Kazakhstan are under discussion.


safflower, breeding, fatty acids, DNA markers, allele, oil, tissue culture, microspores, genetic engineering, medicine

Article Details


Chapman M.A., Hvala J., Strever J. and Burke J.M. Population genetic analysis of safflower (Carthamus tinctorius; Asteraceae) reveals a Near Eastern origin and five centers of diversity. American Journal of Botany, 2010, vol. 97, no. 5, рр. 831–840. Available at: Crossref

Ashri A. Evaluation of the germ plasmcollection of Safflower, Carthamus tinctorius L. V. Distribution and regional divergence for morphological characters. Euphytica, 1975, vol. 24, no. 3, рр. 651-659. Available at: Crossref

Jaradat A.A., Shahid M. Patterns of phenotypic variation in a germplasm collection of Carthamus tinctorius L. from the Middle East. Genetic Resources and Crop Evolution, 2006, vol. 53, no. 2, рр. 225–244. Available at: Crossref

Camas N., Esendal E. Estimates of broad-sense heritability for seed yield and yield components of safflower (Carthamus tinctorius L.). Hereditas, 2006, vol. 143, рр. 55-57. Available at: 17362334

Gecgel U., Demirci M., Esendal E. Seed yield, oil content and fatty acids composition of safflower (Carthamus tinctorius L.) varieties sown in spring and winter. International Journal of Molecular Sciences, 2007, vol. 1, рр. 11–15.

Pooran Golkar, Ahmad Arzani, Abdolmajid M. Rezaei. Genetic Variation in Safflower (Carthamus tinctorious L.) for Seed Quality-Related Traits and Inter-Simple Sequence Repeat (ISSR) Markers. International Journal of Molecular Sciences, 2011, vol. 12, no. 4, рр. 2664-2677.

Available at: Crossref

Osorio J., Fernandez-Martinez J., Mancha M., Garcés R. Mutant sunflowers with high concentration of saturated fatty acids in the oil. Crop Sci., 1995, vol. 35, рр. 739-742. Available at: Crossref

Gyulai J. Market outlook for safflower. In Mündel H.-H., Braun J., and Daniels C., eds. Proceedings of North American Safflower. Conference, Great Falls, Montana, Lethbridge, AB, 1996, рр. 15.

Mahboobeh Vosoughkia, Mehrdad Ghavamib, Maryam Gharachorloo, Mohammad Sharrifmoghaddasi, Amir Hasan Omidi. Lipid Composition and Oxidative Stability of Oils in Safflower (Carthamus tinctorius L.) Seed Varieties Grownin Iran. Advancesin Environmental Biology, 2011, vol. 5, no. 5, рр. 897-902.

Knowles P.F. Safflower. Oil Crops of the World (Röbbelen G., Downey R.K. and Ashri A., eds.). New York: McGraw-Hill, 1989, рр. 363-374.

Knowles P.F. The plant geneticist’s contribution towards changing lipid and amino acid composition of safflower. J. Am. Oil Chem. Soc., 1972, vol. 49, no. 1, рр. 27-29.

Ladd S.L. and Knowles P.F. Inheritance of alleles at two loci regulating fatty acid composition of the seed oil of safflower (Carthamus tinctorius L.). CropSci., 1971, vol. 11, no. 5, рр. 681-684.

Li Dajue and H.-Henning Mündel. Safflower, Carthamus tinctorius L. International Plant Genetic Resources Institute, 1996, 83 p.

Bartholomew S.B. SSRs Temperature effects on the fatty acid composition of developing seeds in safflower, Carthamus tinctorius S.L. MS. Thesis University of California, Davis, 1971.

Bergman J.W., Flynn C.R., Kott R.W., Hatfield P.G., Van Wagoner H., and Boles J.A. Feedlot performance, carcass composition, and muscle and fat CLA concentrations of lambs fed diets supplemented with safflower seeds. Small Ruminant Research, 2003, vol. 49, рр. 11-17. Available at: Crossref

Weiss E.A. Caster, Sesame and Safflower. An Intertext Pub., 1971, 53 p.

Vosoughkia M., Hossainchi Ghareaghag L., Ghavami M., Gharachorloo M., Delkhosh B. Evaluation of Oil Content and Fatty Acid Composition in Seeds of Different Genotypes of Safflower (Carthamus tinctorius L.). International Journal of Agricultural Science and Research, 2011, Serial #2, vol. 2, no. 1.

Ensiye A., Razmjoo K. Effect of Irrigation Regimes on Oil Content and Composition of Safflower (Carthamus tinctorius L.) Cultivars. Journal of the American Oil Chemists' Society, 2010, vol. 87, рр. 499–506. Available at: Crossref

Hans-HenningMündel. Major achievements in safflower breeding and future challenges. 7th International Safflower Conference. WaggaWagga, Australia, 2008.

Velasco L.; Fernández-Martínez J. M. Breeding for oil quality in safflower. // Safflower: a multipurpose species with unexploited potential and world adaptability.- Proceedings of the 5th International Safflower Conference.Williston, North Dakota and Sidney, Montana, USA, 23-27 July, 2001, рр. 133-137.

Jajarmi V. Effect of water stress on germination indices in seven safflower cultivars (Carthamus tinctorius L.). 7th International safflower conference. WaggaWagga, Australia, 2008, 3 to 6 November.

Ashkani J., Pakniyat H., Ghotbi V. Genetic evaluation of several physiological traits for screening of Suitable spring safflower (Carthamus tinctorius L.) genotypes under stress and non-stress irrigation regimes. Pakistan Journal of Biological Science, 2007, vol. 10, no. 14, рр. 2320-2326. Available at: 19070151

Ejaz Hussain Siddiqi, Muhammad Ashraf, Nudrat Aisha Akram. Variation in seed germination and seedling growth in some diverse lines of safflower (Carthamus tinctorius L.) under salt stress // Pakistan J. Bot.-2007.-Vvol.39.-№ no. 6.-P. рр. 1937-1944.

Ramachandram M., Goud J.V. Genetic Analysis of Seed Yield, Oil Content and Their Components in Safflower (Carthamus tinctorius L.). Theor. Appl. Genet, 1981, vol. 60, no. 3, рр. 191-195. Available at: Crossref

Pahlavani M.H., Mirlohi A.F., Saeidi G. Inheritance of Flower Color and Spininess in Safflower (Carthamus tinctorius L.). Journal of Heredity, 2004, vol. 95, no. 3, рр. 265–267. Available at: 15220395

Sujatha M. Biotechnological interventions for genetic improvement of safflower. 7th International safflower conference. WaggaWagga, Australia, 2008, 3 to 6 November.

Sehgal D. and Raina S.N. Genotyping safflower (Carthamus tinctorius) cultivars by DNA fingerprints. Euphytica, 2005, vol. 146, рр. 67-76. Available at: Crossref

Johnson R.C., Kisha T.J. and Evans M.A. Characterizing safflower germplasm with AFLP molecular markers. Crop Sci., 2007, vol. 47, рр. 1728-1736. Available at: Crossref

Yang Y.X., Wu W., Zheng Y.L., Chen L., Liu R.J. and Huang C.Y. Genetic diversity and relationships among safflower (Carthamus tinctorius L.) analysed by inter-simple sequence repeats (ISSRs). Genet Resour Crop Evol., 2007, vol. 54, рр. 1043-1051. Available at: Crossref

Amini F., Saeidi G. and Arzani A. Study of genetic diversity in safflower genotypes using agro-morphological traits and RAPD markers. Euphytica, 2008, vol. 163, no. 1, рр. 21-30. Available at: Crossref

Khan M.A.,Witzke-Ehbrecht, S.V., Maass B.L. and Becker H.C. Relationships among different geographical groups, agro-morphology, fatty acid composition and RAPD marker diversity in safflower (Carthamus tinctorius). Genet Resour Crop Evol., 2009, vol. 56, no. 1, рр. 19-30. Available at: Crossref

Deepmala Sehgal, Soom Nath Raina. SSRs Genotyping safflower (Carthamus tinctorius) cultivars by DNA fingerprints. Euphytica, 2005, vol. 146, no. 1-2, рр. 67–76. Available at: Crossref

Aslam M. &Hazara G.R. Evaluation of world collection of safflower (Carthamus tinctorius L) for yield and other agronomic characters. In: L. Dajue & H. Yuanzhou (Eds.). Third International Safflower Conference. Beijing, China, 1993, June 9–13, 238 p.

Fang, D.Q. & Roose M.L. Identification of closely related citrus cultivars with inter-simple sequence repeat markers. Theor Appl Genet, 1997, vol. 95, no. 3, рр. 408–417. Available at: Crossref

Hamdan Y.A.S., Velasco L. and Perez-Vich B. Development of SCAR markers linked to male sterility and very high linoleic acid content in safflower. Mol Breed., 2008, vol. 22, no. 3, рр. 385-393. Available at: Crossref

García-Moreno María J., Velasco Leonardo, Fernández-Martínez José M., Pérez-Vich Begoña. Transferability of sunflower microsatellite markers to safflower. 7th International safflower conference. WaggaWagga, Australia, 2008, 3 to 6 November.

Kuleung C., Baenziger P. S.and Dweikat I. Transferability of SSR markers among wheat, rye, and triticale. Theor. Appl. Genet, 2004, vol. 108, no. 6, рр. 1147-1150. Available at: Crossref

Peakall R., Gilmore S., Keys W., Morgante M. and Rafalski A. Cross-species amplification of soybean (Glycine max) simple sequence repeats (SSRs) within the genus and other legume genera: implications for the transferability of SSRs in plants. Mol. Biol. Evol., 1998, vol. 15, no. 10, рр. 1275-1287. Available at: 9787434

Plieske J. and Struss D. Microsatellite markers for genome analysis in Brassica. I.Dev elopment in Brassica napus and abundance in Brassicaceae species. Theor. Appl. Genet, 2001, vol. 102, no. 5, рр. 689−694. Available at: Crossref

Rajendra Prasad B., Khadeer M.A., Seeta P., and Anwar S.Y. In vitro induction of androgenic haploids in Safflower (Carthamus tinctorius L.). Plant Cell Reports, 1991, vol. 10, no. 1, рр. 48-51. Available at: Crossref.

Bal U., Touraev A. Microspore Embryogenesis in Selected Medicinal and Ornamental Species of the Asteraceae. Advances in Haploid Production in Higher Plants. Springer Netherlands, 2009, рр. 219-229. Available at: Crossref

Sujatha M. And Dutta Gupta S. Tissue Culture and Genetic Transformation of Safflower (Carthamus tinctorius L.). S.M. Jain and S. Dutta Gupta (eds.). Biotechnology of Neglected and Underutilized Crops-Springer Science+Business Media Dordrecht, 2013, рр. 297-318. Available at: Crossref

Srinivas Belide, Luch Hac, Surinder P Singh, Allan G Green, Craig C Wood. Agrobacterium-mediated transformation of safflower and the efficient recovery of transgenic plants via grafting. Plant Method., 2011, рр. 7-12. Available at: Crossref

Motamedi Ja., Zebarjadi A., Kahrizi1 D., Salmanian A.H. In vitro propagation and Agrobacterium-mediated transformation of safflower (Carthamus tinctorius L.) using a bacterial mutated aro A gene. Australian Journal Crop Science, 2011, vol. 5, no. 4, рр. 479-486.

Sugimori Y., Funakawa S., Pachikin K. M., Ishida N., Kosaki T. Soil salinity dynamics in irrigated fields and its effects on paddy-based rotation systems in Southern Kazakhstan. Land degradation and development, 2008, vol. 19, no. 3, рр. 305–320. Available at: Crossref

Agentstvo Respubliki Kazahstan po statistike 2013[Kazakhstan Agency for Statistics 2013]. Available at:

Analiticheskaja zapiska k operativnomu otchetu po monitoringu Programmy po razvitiju agropromyshlennogo kompleksa v Respublike Kazahstan na 2010–2014 gody za 2012 god [Analytical note to operational report on the monitoring program for the development of agriculture in the Republic of Kazakhstan for 2010-2014 years for 2012]. Available at: ttp:// ‎

Kazahstan: V Almatinskoj oblasti otkryt zavod po pererabotke soi [Kazakhstan: Almaty oblast open soybean processing plant]. Available at:

Gosudarstvennyj reestr selekcionnyh dostizhenij, dopushhennyh k ispol'zovaniju v Respublike Kazahstan [State register of breeding achievements permitted for use in the Republic of Kazakhstan]. Available at:

Ortaev A.K., Zhamalbekov M.N., Konyrbekov M. Rekomendacija: Kollekcionnye i selekcionnye fondy saflora, racionalnoe ispolzovanie, semenovodstvo i tehnologija vozdelyvanija saflora [M. Recommendation: Collection and breeding funds safflower, rational use, seed and safflower cultivation technology]. Saryagash, 2011, рр. 15.